cDNA Clone SIK3 (Long)

• Synonyms: SIK3
• Plasmid: pFastBac.HTb QSK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-QSK
• Genbank: NM_025164 covers residues 864 – 1369
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGCTGCCGGGGCCGG
GACTGGGGGAGCCGGGCCCGCGGGCCGCCTGCTGCCTCCGCCCGCGCCGG
GGTCCCCAGCCGCCCCCGCTGCCGTGTCCCCTGCGGCCGGCCAGCCGCGT
CCCCCAGCCCCGGCCTCCCGCGGACCCATGCCCGCCCGTATCGGCTACTA
CGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCGGTGGTCAAGCGGG
CCACGCACCTCGTCACCAAGGCCAAGGTTGCTATCAAGATCATAGATAAG
ACCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAAGTTCAAAT
TATGAAGATGCTTTGCCACCCCCATATCATCAGGCTCTACCAGGTTATGG
AGACAGAACGGATGATTTATCTGGTGACAGAATATGCTAGTGGAGGGGAA
ATATTTGACCACCTGGTGGCCCATGGTAGAATGGCAGAAAAGGAGGCACG
TCGGAAGTTCAAACAGATCGTCACAGCTGTCTATTTTTGTCACTGTCGGA
ACATTGTTCATCGTGATTTAAAAGCTGAAAATTTACTTCTGGATGCCAAT
CTGAATATCAAAATAGCAGATTTTGGTTTCAGTAACCTCTTCACTCCTGG
GCAGCTGCTGAAGACCTGGTGTGGCAGCCCTCCCTATGCTGCACCTGAAC
TCTTTGAAGGAAAAGAATATGATGGGCCCAAAGTGGACATCTGGAGCCTT
GGAGTTGTCCTCTACGTGCTTGTGTGCGGTGCCCTGCCATTTGATGGAAG
CACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGAAAGTTCCGCATCC
CATTTTTTATGTCCACAGAATGTGAGCATTTGATCCGCCATATGTTGGTG
TTAGATCCCAATAAGCGCCTCTCCATGGAGCAGATCTGCAAGCACAAGTG
GATGAAGCTAGGGGACGCCGATCCCAACTTTGACAGGTTAATAGCTGAAT
GCCAACAACTAAAGGAAGAAAGACAGGTGGACCCCCTGAATGAGGATGTC
CTCTTGGCCATGGAGGACATGGGACTGGACAAAGAACAGACACTGCAGTC
ATTAAGATCAGATGCCTATGATCACTATAGTGCAATCTACAGCCTGCTGT
GTGATCGACATAAGAGACATAAAACCCTGCGTCTCGGAGCACTTCCTAGC
ATGCCCCGAGCCCTGGCCTTTCAAGCACCAGTCAATATCCAGGCGGAGCA
GGCAGGTACTGCTATGAACATCAGCGTTCCCCAGGTGCAGCTGATCAACC
CAGAGAACCAAATTGTGGAGCCGGATGGGACACTGAATTTGGACAGTGAT
GAGGGTGAAGAGCCTTCCCCTGAAGCATTGGTGCGCTATTTGTCAATGAG
GAGGCACACAGTGGGTGTGGCTGACCCACGCACGGAAGTTATGGAAGATC
TGCAGAAGCTCCTACCTGGCTTTCCTGGAGTCAACCCCCAGGCTCCATTC
CTGCAGGTGGCCCCTAATGTGAACTTCATGCACAACCTGTTGCCTATGCA
AAACTTGCAACCAACCGGGCAACTTGAGTACAAGGAGCAGTCTCTCCTAC
AGCCGCCCACGCTACAGCTGTTGAATGGAATGGGCCCCCTTGGCCGGAGG
GCATCAGATGGAGGAGCCAACATCCAACTGCATGCCCAGCAGCTGCTGAA
GCGCCCACGGGGACCCTCTCCGCTTGTCACCATGACACCAGCAGTGCCAG
CAGTTACCCCTGTGGACGAGGAGAGCTCAGACGGGGAGCCAGACCAGGAA
GCTGTGCAGAGGTACTTGGCAAATAGGTCCAAAAGACATACACTGGCCAT
GACCAACCCTACAGCTGAGATCCCACCGGACCTACAACGGCAGCTAGGAC
AGCAGCCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTGGTACCTGATCAG
CATCGCTCTACCTACAAGGACTCCAACACTCTGCACCTCCCTACGGAGCG
TTTCTCCCCTGTGCGCCGGTTCTCAGATGGGGCTGCGAGCATCCAGGCCT
TCAAAGCTCACCTGGAAAAAATGGGCAACAACAGCAGCATCAAACAGCTG
CAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCAGATTGATGA
AAGAACCCTGGAGAAGACCCAGCAGCAGCATATGTTATACCAGCAGGAGC
AGCACCATCAAATTCTCCAGCAACAAATTCAAGACTCTATCTGTCCTCCT
CAGCCATCTCCACCTCTTCAGGCTGCATGTGAAAATCAGCCAGCCCTCCT
TACCCATCAGCTCCAGAGGTTAAGGATTCAGCCTTCAAGCCCACCCCCCA
ACCACCCCAACAACCATCTCTTCAGGCAGCCCAGTAATAGTCCTCCCCCC
ATGAGCAGTGCCATGATCCAGCCTCACGGGGCTGCATCTTCTTCCCAGTT
TCAAGGCTTACCTTCCCGCAGTGCAATCTTTCAGCAGCAACCTGAGAACT
GTTCCTCTCCTCCCAACGTGGCACTAACCTGCTTGGGTATGCAGCAGCCT
GCTCAGTCACAGCAGGTCACCATCCAAGTCCAAGAGCCTGTTGACATGCT
CAGCAACATGCCAGGCACAGCTGCAGGCTCCAGTGGGCGCGGCATCTCCA
TCAGCCCCAGTGCTGGTCAGATGCAGATGCAGCACCGTACCAACCTGATG
GCCACCCTCAGCTATGGGCACCGTCCCTTGTCCAAGCAGCTGAGTGCTGA
CAGTGCAGAGGCTCACAGCTTGAACGTGAATCGGTTCTCCCCTGCTAACT
ACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGACCAGTCCCGGGGT
TCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTTCTCTCCAACCCA
AGCCCTGAAAGTCCCTCCACTTGACCAATTCCCCACCTTCCCTCCCAGTG
CACATCAGCAGCCGCCACACTATACCACGTCGGCACTACAGCAGGCCCTG
CTGTCTCCCACGCCGCCAGACTATACAAGACACCAGCAGGTACCCCACAT
CCTTCAAGGACTGCTTTCTCCCCGGCATTCGCTCACCGGCCACTCGGACA
TCCGGCTGCCCCCAACAGAGTTTGCACAGCTCATTAAAAGGCAGCAGCAA
CAACGGCAGCAGCAGCAGCAACAGCAGCAACAGCAAGAATACCAGGAACT
GTTCAGGCACATGAACCAAGGGGATGCGGGGAGTCTGGCTCCCAGCCTTG
GGGGACAGAGCATGACAGAGCGCCAGGCTTTATCTTATCAAAATGCTGAC
TCTTATCACCATCACACCAGCCCCCAGCATCTGCTACAAATCAGGGCACA
AGAATGTGTCTCACAGGCTTCCTCACCCACCCCGCCCCACGGGTATGCTC
ACCAGCCGGCACTGATGCATTCAGAGAGCATGGAGGAGGACTGCTCGTGT
GAGGGGGCCAAGGATGGCTTCCAAGACAGTAAGAGTTCAAGTACATTGAC
CAAAGGTTGCCATGACAGCCCTCTGCTCTTGAGTACCGGTGGACCTGGGG
ACCCTGAATCTTTGCTAGGAACTGTGAGTCATGCCCAAGAATTGGGGATA
CATCCCTATGGTCATCAGCCAACTGCTGCATTCAGTAAAAATAAGGTGCC
CAGCAGAGAGCCTGTCATAGGGAACTGCATGGATAGAAGTTCTCCAGGAC
AAGCAGTGGAGCTGCCGGATCACAATGGGCTCGGGTACCCAGCACGCCCC
TCCGTCCATGAGCACCACAGGCCCCGGGCCCTCCAGAGACACCACACGAT
CCAGAACAGCGACGATGCTTATGTACAGCTGGATAACTTGCCAGGAATGA
GTCTCGTGGCTGGGAAAGCACTTAGCTCTGCCCGGATGTCGGATGCAGTT
CTCAGTCAGTCTTCGCTCATGGGCAGCCAGCAGTTTCAGGATGGGGAAAA
TGAGGAATGTGGGGCAAGCCTGGGAGGTCATGAGCACCCAGACCTGAGTG
ATGGCAGCCAGCATTTAAACTCCTCTTGCTATCCATCTACGTGTATTACA
GACATTCTGCTCAGCTACAAGCACCCCGAAGTCTCCTTCAGCATGGAGCA
GGCAGGCGTGTAAggatcc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSAAAAASGAGGAAGAGTGGAGPAGRLLPPPAPGSPAAPAAVSPAAGQPRPPAPASRGPMPARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKEERQVDPLNEDVLLAMEDMGLDKEQTLQSLRSDAYDHYSAIYSLLCDRHKRHKTLRLGALPSMPRALAFQAPVNIQAEQAGTAMNISVPQVQLINPENQIVEPDGTLNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTEVMEDLQKLLPGFPGVNPQAPFLQVAPNVNFMHNLLPMQNLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGRRASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEAVQRYLANRSKRHTLAMTNPTAEIPPDLQRQLGQQPFRSRVWPPHLVPDQHRSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMGNNSSIKQLQQECEQLQKMYGGQIDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQAACENQPALLTHQLQRLRIQPSSPPPNHPNNHLFRQPSNSPPPMSSAMIQPHGAASSSQFQGLPSRSAIFQQQPENCSSPPNVALTCLGMQQPAQSQQVTIQVQEPVDMLSNMPGTAAGSSGRGISISPSAGQMQMQHRTNLMATLSYGHRPLSKQLSADSAEAHSLNVNRFSPANYDQAHLHPHLFSDQSRGSPSSYSPSTGVGFSPTQALKVPPLDQFPTFPPSAHQQPPHYTTSALQQALLSPTPPDYTRHQQVPHILQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQQQRQQQQQQQQQQEYQELFRHMNQGDAGSLAPSLGGQSMTERQALSYQNADSYHHHTSPQHLLQIRAQECVSQASSPTPPHGYAHQPALMHSESMEEDCSCEGAKDGFQDSKSSSTLTKGCHDSPLLLSTGGPGDPESLLGTVSHAQELGIHPYGHQPTAAFSKNKVPSREPVIGNCMDRSSPGQAVELPDHNGLGYPARPSVHEHHRPRALQRHHTIQNSDDAYVQLDNLPGMSLVAGKALSSARMSDAV
LSQSSLMGSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCIT
DILLSYKHPEVSFSMEQAGV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU1274
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; The sequence of this clone refers to an older reference sequence with an additional 59 amino acids at the N terminus.