cDNA Clone Rad6

• Plasmid: pMalP Rad6
• Parent Plasmid: pMAL6P
• Expressed: MBP-Rad6
• Genbank: NM_003337
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGTCGACCCCGGCCCGGAGGAGGCTCATGCGGGATTTCAAGCG
GTTACAAGAGGACCCACCTGTGGGTGTCAGTGGCGCACCATCTGAAAACA
ACATCATGCAGTGGAATGCAGTTATATTTGGACCAGAAGGGACACCTTTT
GAAGATGGTACTTTTAAACTAGTAATAGAATTTTCTGAAGAATATCCAAA
TAAACCACCAACTGTTAGGTTTTTATCCAAAATGTTTCATCCAAATGTGT
ATGCTGATGGTAGCATATGTTTAGATATCCTTCAGAATCGATGGAGTCCA
ACATATGATGTATCTTCTATCTTAACATCAATTCAGTCTCTGCTGGATGA
ACCGAATCCTAACAGTCCAGCCAATAGCCAGGCAGCACAGCTTTATCAGG
AAAACAAACGAGAATATGAGAAAAGAGTTTCGGCCATTGTTGAACAAAGC
TGGAATGATTCATAAGCGGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVG
KKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSG
LLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPN
PPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENG
KYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAM
TINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENA
QKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNN
NNNNNNLGIEGRISELALEVLFQGPWGSMSTPARRRLMRDFKRLQEDPPV
GVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRF
LSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPA
NSQAAQLYQENKREYEKRVSAIVEQSWNDS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20007
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express Rad 6 with N-terminal MBP tag (contains preScission protease site); ; Estimated MW of expressed protein : 64054 Daltons