cDNA Clone OTUD4

• Plasmid: pET156P OTUD4 Isoform 3
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His OTUD4
• Genbank: NP_001096123.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCCTGTATTCACTATCTTCGAGAGAACAGAGAGAAATTTGAAGCGTTTATAGAAGGATCATTTGAAGAATATTTAAAGCGTTTGGAAAATCCACAGGAATGGGTAGGACAAGTGGAAATAAGTGCCCTTTCTCTTATGTACAGGAAAGATTTTATAATTTATCGGGAACCAAATGTTTCTCCTTCACAAGTAACAGAAAATAATTTTCCTGAAAAGGTGTTACTGTGTTTTTCAAATGGAAATCATTATGATATTGTGTATCCCATAAAGTATAAAGAAAGCTCTGCTATGTGTCAGTCTCTCCTTTATGAATTGCTGTATGAGAAGGTATTTAAAACTGATGTTAGTAAAATTGTGATGGAACTAGACACGTTGGAAGTAGCTGATGAAGATAACAGTGAAATATCAGATTCAGAGGATGACAGTTGCAAGAGTAAGACTGCTGCTGCTGCTGCTGATGTGAATGGATTTAAACCTTTGTCAGGCAATGAGCAGCTGAAGAACAATGGGAACTCTACTAGCCTGCCTTTGTCTAGAAAGGTTCTTAAGTCACTCAATCCTGCAGTCTATAGAAATGTGGAATATGAAATTTGGCTGAAGTCTAAACAAGCTCAGCAAAAACGTGATTATTCCATTGCTGCTGGCTTACAATATGAAGTTGGAGACAAATGTCAAGTTAGGTTGGATCACAATGGAAAATTTTTGAATGCAGATGTTCAAGGAATTCATTCTGAGAATGGACCAGTTTTGGTTGAAGAACTGGGAAAGAAGCACACATCAAAGAACCTCAAGGCACCTCCCCCAGAAAGCTGGAACACAGTGTCAGGGAAGAAGATGAAAAAACCTTCCACTTCTGGACAAAATTTCCATTCTGATGTGGATTACAGAGGGCCAAAGAATCCAAGCAAGCCAATAAAAGCCCCATCAGCACTACCTCCTCGACTGCAGCATCCTTCAGGAGTAAGACAACATGCGTTCTCTAGTCATTCTTCAGGGTCACAGTCTCAGAAATTCTCCAGTGAGCACAAAAATCTTAGCCGGACACCTTCACAGATCATAAGAAAACCTGATCGTGAAAGAGTTGAGGATTTTGATCACACAAGTCGAGAATCTAACTATTTCGGCCTTTCCCCAGAAGAGCGCAGAGAGAAGCAAGCTATAGAAGAATCCCGTTTACTCTATGAGATTCAGAACAGAGATGAACAGGCTTTCCCAGCCCTTTCCAGCTCATCAGTCAATCAGTCAGCTTCTCAGAGTAGCAATCCATGTGTCCAGAGAAAATCATCACATGTAGGTGATAGAAAAGGAAGCAGGCGGAGAATGGATACAGAAGAACGAAAAGACAAAGACTCTATTCATGGACATAGTCAGTTGGATAAAAGACCCGAACCAAGCACATTGGAGAATATTACTGATGATAAATATGCAACAGTTTCATCACCATCAAAGTCAAAGAAGTTAGAGTGCCCTTCTCCTGCGGAACAAAAGCCAGCAGAACATGTGTCTTTGTCAAATCCAGCTCCCCTTCTAGTTTCTCCAGAGGTACATCTAACTCCTGCGGTGCCTTCTTTACCAGCCACTGTGCCAGCCTGGCCAAGTGAACCTACAACTTTTGGACCAACAGGTGTCCCTGCTCCAATTCCCGTTTTGTCAGTGACACAGACTTTGACCACTGGACCTGATTCAGCTGTATCCCAAGCTCATTTAACACCCTCTCCAGTTCCTGTGTCAATACAGGCAGTTAACCAGCCCTTGATGCCTTTGCCTCAGACATTGAGCCTTTATCAAGACCCACTCTATCCTGGGTTTCCTTGTAATGAAAAGGGAGATCGAGCCATTGTACCACCTTATTCACTGTGTCAGACTGGGGAGGACCTACCTAAAGATAAGAATATTCTTCGATTCTTCTTCAATCTTGGTGTGAAGGCATACAGTTGTCCTATGTGGGCCCCACATTCTTACCTGTACCCTCTGCACCAGGCCTACCTGGCAGCCTGCAGGATGTACCCAAAGGTCCCTGTCCCTGTTTATCCTCATAATCCCTGGTTCCAAGAGGCTCCTGCTGCTCAGAATGAAAGTGATTGTACCTGTACTGATGCCCACTTTCCTATGCAGACTGAGGCCAGTGTTAATGGTCAAATGCCACAGCCAGAGATTGGACCGCCGACATTTTCTTCACCTCTGGTTATCCCTCCATCTCAGGTGTCTGAAAGTCATGGACAATTGTCTTACCAGGCTGATCTTGAATCTGAGACCCCTGGGCAGCTTCTGCATGCTGATTATGAAGAGTCACTAAGTGGCAAGAATATGTTCCCCCAGCCATCTTTTGGACCCAATCCATTCTTAGGCCCAGTTCCTATTGCACCTCCTTTCTTTCCTCATGTTTGGTATGGGTACCCTTTTCAGGGATTCATAGAAAATCCAGTAATGAGGCAGAATATTGTCCTGCCCTCTGATGAAAAAGGAGAATTGGATCTGTCTCTGGAAAATCTGGATCTGTCTAAAGATTGTGGTTCAGTTTCAACAGTAGATGAGTTTCCAGAAGCCAGGGGTGAACATGTACATTCTCTCCCTGAAGCAAGTGTGAGCAGTAAGCCGGACGAAGGCCGGACAGAGCAATCTTCCCAGACACGAAAGGCAGATACGGCATTGGCTTCCATCCCTCCTGTAGCAGAGGGAAAGGCTCATCCTCCCACTCAGATTCTAAACAGAGAGAGAGAAACTGTGCCTGTTGAACTTGAACCTAAAAGGACCATTCAAAGCCTGAAAGAAAAAACAGAAAAAGTAAAAGATCCTAAGACTGCTGCTGATGTGGTCAGCCCTGGGGCCAACTCTGTTGATAGCAGAGTGCAAAGACCAAAAGAAGAGAGTTCAGAAGATGAAAATGAAGTGTCTAATATTTTGAGAAGTGGTAGATCCAAGCAGTTCTATAATCAAACTTATGGAAGCAGGAAGTACAAAAGTGATTGGGGCTATTCTGGTAGGGGTGGATATCAACATGTGAGAAGTGAGGAGTCCTGGAAAGGACAGCCAAGCAGAAGTCGGGATGAAGGTTATCAGTACCATCGAAATGTCAGAGGGCGACCATTTAGGGGAGATAGGAGGAGATCAGGGATGGGAGATGGCCATAGGGGACAGCACACTTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMACIHYLRENREKFEAFIEGSFEEYLKRLENPQEWVGQVEISALSLMYRKDFIIYREPNVSPSQVTENNFPEKVLLCFSNGNHYDIVYPIKYKESSAMCQSLLYELLYEKVFKTDVSKIVMELDTLEVADEDNSEISDSEDDSCKSKTAAAAADVNGFKPLSGNEQLKNNGNSTSLPLSRKVLKSLNPAVYRNVEYEIWLKSKQAQQKRDYSIAAGLQYEVGDKCQVRLDHNGKFLNADVQGIHSENGPVLVEELGKKHTSKNLKAPPPESWNTVSGKKMKKPSTSGQNFHSDVDYRGPKNPSKPIKAPSALPPRLQHPSGVRQHAFSSHSSGSQSQKFSSEHKNLSRTPSQIIRKPDRERVEDFDHTSRESNYFGLSPEERREKQAIEESRLLYEIQNRDEQAFPALSSSSVNQSASQSSNPCVQRKSSHVGDRKGSRRRMDTEERKDKDSIHGHSQLDKRPEPSTLENITDDKYATVSSPSKSKKLECPSPAEQKPAEHVSLSNPAPLLVSPEVHLTPAVPSLPATVPAWPSEPTTFGPTGVPAPIPVLSVTQTLTTGPDSAVSQAHLTPSPVPVSIQAVNQPLMPLPQTLSLYQDPLYPGFPCNEKGDRAIVPPYSLCQTGEDLPKDKNILRFFFNLGVKAYSCPMWAPHSYLYPLHQAYLAACRMYPKVPVPVYPHNPWFQEAPAAQNESDCTCTDAHFPMQTEASVNGQMPQPEIGPPTFSSPLVIPPSQVSESHGQLSYQADLESETPGQLLHADYEESLSGKNMFPQPSFGPNPFLGPVPIAPPFFPHVWYGYPFQGFIENPVMRQNIVLPSDEKGELDLSLENLDLSKDCGSVSTVDEFPEARGEHVHSLPEASVSSKPDEGRTEQSSQTRKADTALASIPPVAEGKAHPPTQILNRERETVPVELEPKRTIQSLKEKTEKVKDPKTAADVVSPGANSVDSRVQRPKEESSEDENEVSNILRSGRSKQFYNQTYGSRKYKSDWGYSGRGGYQHVRSEESWKGQPSRSRDEGYQYHRNVRGRPFRGDRRRSGMGDGHRGQHT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU21248
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 143791 Daltons