cDNA Clone slit-robo-GAP 1

• Plasmid: pCMV5-HA SRGAP1
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-Slit-Robo-GAP1 isoform 1
• Genbank: BC053903.1 GI:31753110
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGTCCACCCCGAGCCGATTCAAGAAGGACAAAGAGATCATAGC
CGAGTATGAAAGTCAAGTCAAAGAAATTCGAGCTCAACTGGTAGAACAAC
AAAAATGCCTGGAGCAGCAAACGGAGATGCGAGTTCAGCTTCTCCAGGAT
CTGCAAGATTTCTTCCGAAAAAAAGCTGAAATTGAGACGGAATATTCCCG
GAATCTAGAGAAGTTAGCAGAAAGGTTCATGGCAAAAACAAGAAGCACTA
AGGATCATCAACAATACAAGAAAGACCAGAACCTGTTGTCTCCAGTGAAC
TGCTGGTATTTGCTCCTGAACCAAGTAAGGAGAGAAAGCAAAGACCATGC
AACCTTGAGTGACATCTATCTGAACAATGTGATTATGCGGTTCATGCAGA
TAAGTGAGGATTCTACCAGGATGTTTAAAAAGAGCAAAGAGATTGCATTC
CAACTTCATGAGGATTTAATGAAGGTTCTTAATGAGCTTTATACGGTGAT
GAAAACATACCATATGTATCATGCAGAGAGCATCAGTGCAGAGAGCAAGC
TGAAAGAGGCCGAAAAACAAGAGGAAAAGCAAATTGGGAGATCTGGTGAT
CCAGTCTTCCATATTCGACTAGAGGAGAGACATCAACGGCGAAGCTCTGT
AAAGAAAATTGAAAAAATGAAAGAAAAAAGACAAGCAAAATATTCAGAAA
ATAAGCTAAAATCAATTAAGGCACGGAACGAATATCTCCTAACACTTGAA
GCCACCAATGCCTCAGTTTTCAAGTACTATATTCATGATCTTTCTGATTT
AATTGATTGCTGTGATCTTGGCTACCATGCAAGTCTGAACAGAGCCCTAA
GAACATATCTGTCTGCGGAGTACAACCTTGAAACCTCCAGACATGAGGGC
TTAGACATTATTGAGAATGCAGTTGATAATTTAGAGCCCAGGAGCGATAA
GCAGAGATTCATGGAGATGTACCCTGCTGCGTTCTGTCCACCAATGAAGT
TTGAGTTTCAGTCTCACATGGGTGATGAGGTGTGCCAGGTCAGTGCCCAG
CAGCCAGTCCAGGCAGAGCTCATGCTCAGGTACCAACAGTTGCAGTCCCG
CCTTGCCACGCTCAAAATCGAGAATGAAGAGGTTAAGAAAACGACTGAAG
CCACCTTGCAGACGATACAAGATATGGTCACCATCGAGGACTATGATGTT
TCTGAATGCTTCCAGCACAGTCGTTCCACAGAATCTGTGAAGTCCACTGT
CTCTGAAACCTACCTGAGTAAACCCAGCATCGCCAAGAGAAGAGCCAACC
AGCAGGAAACTGAACAGTTCTACTTCATGAAACTCAGAGAATATTTGGAA
GGCAGTAATCTCATCACAAAACTTCAAGCCAAACATGACTTGCTGCAGAG
GACCCTGGGAGAAGGTCATAGAGCTGAATATATGACTACAAGGCCTCCAA
ATGTTCCCCCTAAGCCCCAGAAACACAGGAAGTCCAGGCCCCGCTCACAG
TATAATACTAAGTTGTTTAATGGGGATTTGGAAACATTCGTCAAGGACTC
AGGACAGGTTATTCCCCTCATTGTGGAAAGCTGTATTCGGTTCATCAATC
TCTATGGTCTTCAGCATCAGGGGATTTTCAGAGTGTCTGGTTCCCAGGTG
GAAGTCAATGATATTAAAAATTCATTTGAGAGAGGTGAAAATCCTTTGGC
TGATGACCAGAGTAACCATGATATTAACTCAGTTGCTGGCGTTCTGAAGC
TCTATTTCCGTGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGATTTAAC
GATCTGATTTCTTGTATCAGAATAGATAATCTCTATGAGAGGGCGCTTCA
CATCCGCAAACTCCTCCTGACTTTGCCCAGGTCGGTCCTTATAGTGATGA
GGTACCTCTTTGCCTTCCTCAATCATCTATCACAGTACAGCGATGAGAAT
ATGATGGACCCTTATAACCTGGCCATTTGCTTTGGCCCAACATTGATGCC
TGTCCCAGAAATACAGGATCAAGTGTCTTGCCAGGCACATGTGAATGAAA
TTATCAAAACCATCATCATCCACCATGAGACTATTTTCCCAGATGCTAAA
GAGCTGGATGGCCCTGTTTATGAGAAATGTATGGCTGGAGATGACTATTG
CGACAGCCCATACAGTGAGCACGGTACATTGGAGGAAGTGGACCAAGATG
CTGGTACAGAGCCCCACACAAGTGAAGATGAATGTGAGCCAATAGAAGCA
ATAGCCAAGTTTGACTATGTTGGGCGGTCCGCCAGAGAACTATCCTTCAA
GAAGGGTGCCTCCCTGCTGCTGTATCACCGTGCATCTGAGGACTGGTGGG
AAGGCAGGCACAACGGGATTGACGGGCTGGTGCCTCACCAGTATATAGTG
GTGCAGGATATGGATGATACGTTTTCAGACACTCTGAGCCAAAAAGCCGA
CAGTGAGGCCAGCAGTGGGCCAGTCACGGAAGACAAGTCCTCATCCAAGG
ACATGAACTCCCCGACAGACCGTCATCCTGACGGCTATTTAGCCAGGCAA
CGAAAAAGAGGAGAGCCACCCCCTCCAGTAAGGCGTCCTGGCAGGACCAG
TGATGGCCATTGCCCGCTCCACCCTCCACATGCCCTTTCTAACTCCTCAG
TTGACCTAGGGTCCCCAAGCCTTGCCAGTCACCCCCGGGGCCTGCTGCAG
AACCGTGGCCTCAACAATGACAGTCCTGAGCGGAGGCGCAGGCCTGGCCA
TGGCAGCCTGACCAACATCAGCCGGCACGACTCCCTCAAGAAGATCGACA
GCCCTCCCATTAGAAGGTCCACGTCATCAGGGCAATACACGGGCTTCAAT
GACCACAAGCCACTGGACCCAGAGACAATTGCTCAGGATATTGAAGAAAC
GATGAACACAGCTTTGAATGAACTCCGAGAACTGGAGAGACAGAGCACAG
CAAAGCATGCCCCTGATGTGGTGCTGGATACCCTGGAGCAAGTGAAAAAC
TCTCCCACCCCTGCCACTTCCACGGAATCTCTCAGCCCTTTGCACAACGT
TGCCCTCAGGAGCTCCGAGCCTCAGATTCGACGTAGCACGAGCTCCTCCA
GTGACACAATGAGTACTTTCAAGCCTATGGTGGCACCCAGAATGGGCGTG
CAGCTGAAGCCTCCAGCCCTTAGGCCAAAACCTGCTGTTCTTCCAAAAAC
AAATCCTACCATAGGACCTGCCCCACCTCCCCAGGGTCCAACAGACAAGT
CATGCACAATGTAAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQ
TEMRVQLLQDLQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQYK
KDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTR
MFKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQ
EEKQIGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIK
ARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAE
YNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHM
GDEVCQVSAQQPVQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQ
DMVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQF
YFMKLREYLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTTRPPNVPPKPQ
KHRKSRPRSQYNTKLFNGDLETFVKDSGQVIPLIVESCIRFINLYGLQHQ
GIFRVSGSQVEVNDIKNSFERGENPLADDQSNHDINSVAGVLKLYFRGLE
NPLFPKERFNDLISCIRIDNLYERALHIRKLLLTLPRSVLIVMRYLFAFL
NHLSQYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIIKTIII
HHETIFPDAKELDGPVYEKCMAGDDYCDSPYSEHGTLEEVDQDAGTEPHT
SEDECEPIEAIAKFDYVGRSARELSFKKGASLLLYHRASEDWWEGRHNGI
DGLVPHQYIVVQDMDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTD
RHPDGYLARQRKRGEPPPPVRRPGRTSDGHCPLHPPHALSNSSVDLGSPS
LASHPRGLLQNRGLNNDSPERRRRPGHGSLTNISRHDSLKKIDSPPIRRS
TSSGQYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQSTAKHAPDV
VLDTLEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTF
KPMVAPRMGVQLKPPALRPKPAVLPKTNPTIGPAPPPQGPTDKSCTM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7138
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express human SRGAP1 with N-terminal HA tag.; ; Estimated MW of expressed protein : 125620 Daltons