cDNA Clone SEPT1

• Synonyms: septin 1
• Plasmid: pGEX6P-SEPT1
• Parent Plasmid: pGEX6P1
• Expressed: GST-SEPT1
• Genbank: NP_443070 [gi:16604248]
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGACAAGGAGTACGTGGGTTTTGCTGCCCTCCCCAACCAGCT
GCACCGCAAGTCTGTCAAGAAGGGGTTTGACTTCACGCTAATGGTGGCAG
GGGAGTCAGGCCTAGGGAAATCCACCCTCATCAACAGCCTCTTCCTCACC
AACCTCTATGAGGATCGCCAGGTGCCAGAGGCCAGTGCTCGCTTGACACA
GACCCTGGCCATTGAGCGCCGGGGCGTAGAGATTGAGGAAGGGGGTGTGA
AAGTGAAGCTGACCCTTGTGGACACACCTGGCTTTGGGGACTCAGTGGAC
TGCTCTGACTGCTGGCTTCCGGTGGTGAAATTCATCGAGGAGCAATTTGA
GCAGTACCTTAGGGATGAGAGTGGCCTGAACCGGAAGAACATCCAGGACT
CCCGAGTCCACTGCTGCCTCTACTTCATCTCACCCTTCGGCCGGGGGCTC
CGGCCCCTAGATGTGGCCTTCCTCCGGGCAGTACACGAGAAAGTCAACAT
CATCCCAGTCATTGGCAAAGCGGATGCTCTGATGCCCCAGGAAACCCAGG
CCCTCAAGCAGAAGATCCGGGATCAGTTGAAGGAAGAGGAGATCCACATC
TACCAGTTCCCCGAATGTGACTCTGATGAAGATGAAGACTTCAAGAGGCA
GGATGCAGAGATGAAGGAAAGCATCCCTTTTGCAGTCGTGGGATCATGCG
AGGTGGTGAGGGATGGCGGGAACCGGCCGGTGAGGGGACGCCGCTACTCC
TGGGGGACCGTGGAGGTGGAGAACCCACATCACTGCGATTTCCTGAACCT
GCGACGGATGCTGGTGCAGACACACCTGCAGGACCTGAAAGAGGTGACGC
ACGATCTGCTCTACGAGGGCTACCGGGCCCGCTGCCTACAGAGCCTGGCC
CGGCCTGGGGCTCGCGATCGAGCCAGCCGCAGTAAGCTTTCCCGCCAGAG
CGCCACAGAGATCCCGCTGCCCATGCTGCCTCTGGCGGACACCGAGAAGC
TGATCCGCGAGAAAGACGAAGAGCTGCGCCGCATGCAAGAGATGCTGGAG
AAGATGCAGGCCCAAATGCAGCAGAGCCAGGCCCAGGGCGAGCAGTCAGA
CGCCCTCTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDKEYVGFAALPNQLHRKS
VKKGFDFTLMVAGESGLGKSTLINSLFLTNLYEDRQVPEASARLTQTLAI
ERRGVEIEEGGVKVKLTLVDTPGFGDSVDCSDCWLPVVKFIEEQFEQYLR
DESGLNRKNIQDSRVHCCLYFISPFGRGLRPLDVAFLRAVHEKVNIIPVI
GKADALMPQETQALKQKIRDQLKEEEIHIYQFPECDSDEDEDFKRQDAEM
KESIPFAVVGSCEVVRDGGNRPVRGRRYSWGTVEVENPHHCDFLNLRRML
VQTHLQDLKEVTHDLLYEGYRARCLQSLARPGARDRASRSKLSRQSATEI
PLPMLPLADTEKLIREKDEELRRMQEMLEKMQAQMQQSQAQGEQSDAL

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU7012
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express SEPT1 with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 68793 Daltons