cDNA Clone ROCK2

• Synonyms: ROCK2
• Plasmid: pGEX6P-1-ROCK2 1-542
• Parent Plasmid: pGEX6P1
• Expressed: GST-ROCK2 1-542
• Genbank: NM_004850
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGAGCC
GGCCCCCGCCGACGGGGAAAATGCCCGGCGCCCCCGAGACCGCGCCGGGG
GACGGGGCAGGCGCGAGCCGCCAGAGGAAGCTGGAGGCGCTGATCCGAGA
CCCTCGCTCCCCCATCAACGTGGAGAGCTTGCTGGATGGCTTAAATTCCT
TGGTCCTTGATTTAGATTTTCCTGCTTTGAGGAAAAACAAGAACATAGAT
AATTTCTTAAATAGATATGAGAAAATTGTGAAAAAAATCAGAGGTCTACA
GATGAAGGCAGAAGACTATGATGTTGTAAAAGTTATTGGAAGAGGTGCTT
TTGGTGAAGTGCAGTTGGTTCGTCACAAGGCATCGCAGAAGGTTTATGCT
ATGAAGCTTCTTAGTAAGTTTGAAATGATAAAAAGATCAGATTCTGCCTT
TTTTTGGGAAGAAAGAGATATTATGGCCTTTGCCAATAGCCCCTGGGTGG
TTCAGCTTTTTTATGCCTTTCAAGATGATAGGTATCTGTACATGGTAATG
GAGTACATGCCTGGTGGAGACCTTGTAAACCTTATGAGTAATTATGATGT
GCCTGAAAAATGGGCCAAATTTTACACTGCTGAAGTTGTTCTTGCTCTGG
ATGCAATACACTCCATGGGTTTAATACACAGAGATGTGAAGCCTGACAAC
ATGCTCTTGGATAAACATGGACATCTAAAATTAGCAGATTTTGGCACGTG
CATGAAGATGGATGAAACAGGCATGGTACATTGTGATACAGCAGTTGGAA
CACCGGATTATATATCACCTGAGGTTCTGAAATCACAAGGGGGTGATGGT
TTCTATGGGCGAGAATGTGATTGGTGGTCTGTAGGTGTTTTCCTTTATGA
GATGCTAGTGGGGGATACTCCATTTTATGCGGATTCACTTGTAGGAACAT
ATAGCAAAATTATGGATCATAAGAATTCACTGTGTTTCCCTGAAGATGCA
GAAATTTCCAAACATGCAAAGAATCTCATCTGTGCTTTCTTAACAGATAG
GGAGGTACGACTTGGGAGAAATGGGGTGGAAGAAATCAGACAGCATCCTT
TCTTTAAGAATGATCAGTGGCATTGGGATAACATAAGAGAAACGGCAGCT
CCTGTAGTACCTGAACTCAGCAGTGACATAGACAGCAGCAATTTCGATGA
CATTGAAGATGACAAAGGAGATGTAGAAACCTTCCCAATTCCTAAAGCTT
TTGTTGGAAATCAGCTGCCTTTCATCGGATTTACCTACTATAGAGAAAAT
TTATTATTAAGTGACTCTCCATCTTGTAGAGAAACTGATTCCATACAATC
AAGGAAAAATGAAGAAAGTCAAGAGATTCAGAAAAAACTGTATACATTAG
AAGAACATCTTAGCAATGAGATGCAAGCCAAAGAGGAACTGGAACAGAAG
TGCAAATCTGTTAATACTCGCCTAGAAAAAACAGCAAAGGAGCTAGAAGA
GGAGATTACCTTACGGAAAAGTGTGGAATCAGCATTAAGACAGTTAGAAA
GAGAAAAGGCGCTTCTTCAGCACAAAAATGCAGAATATCAGAGGAAAGCT
GATCATGAAGCAGACAAAAAACGAAATTTGGAAAATGATGTTAACAGCTT
AAAAGATCAACTTGAAGATTTGAAAAAAAGAAATCAAAACTCTCAAATAT
AAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSRPPPTGKMPGAPETAPG
DGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNID
NFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYA
MKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVM
EYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDN
MLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDG
FYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDA
EISKHAKNLICAFLTDREVRLGRNGVEEIRQHPFFKNDQWHWDNIRETAA
PVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYREN
LLLSDSPSCRETDSIQSRKNEESQEIQKKLYTLEEHLSNEMQAKEELEQK
CKSVNTRLEKTAKELEEEITLRKSVESALRQLEREKALLQHKNAEYQRKA
DHEADKKRNLENDVNSLKDQLEDLKKRNQNSQI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4800
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.