cDNA Clone SARP1

• Plasmid: pEBG6P-1 SARP1 F357A
• Parent Plasmid: pEBG6P
• Expressed: GST-SARP1 F357A
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCATCGATGCGGCCGCGGACCGCGGCTAC
GCAGCCAGCGACTCCTCTGGTGTGAGCGGCAGTGAAGACGCCAGCTACCG
CTTCAGTGGCTTTTGGGAGAGAGAAAGTGAAGACGAAGGTTTCCGCTGCA
GCTTCTGGGAGAGAGCAAGAGAGGACCTTGGGCCCCGTCCTAGTGACGAC
GGAGAAGAGGGCCGCTGCCGCTGCAGTGGCTCGTGGGTGAGAGCAAGTGA
AGACCGCCGCAGCATCAGGGGCCTGGACTCAACTCCTCCCCAGAGTCGGA
GGTGTTGCGCCATGCCCGGGGTGGCCAATTCAGGCCCCTCCACTTCCTCT
AGGGAGACTGCAAACCCCTGTTCCAGGAAGAAGGTGCATGCTGGCAGCAT
ACATGATGCAGTACGAGCTGGAGATGTAAAGCAGCTTTCAGAAATAGTGG
TACGTGGAGCCAGCATTAATGAACTTGATGTTCTCCATAAGTTTACCCCT
TTACATTGGGCAGCACATTCTGGAAGTTTGGAGTGTCTTCATTGGCTGCT
CTGGCATGGAGCTGATATCACACACGTAACAACGAGAGGTTGGACAGCAT
CTCACATAGCTGCAATCAGGGGTCAGGATGCTTGTGTACAGGCTCTTATA
ATGAATGGAGCAAATCTGACAGCCCAGGATGACCGGGGATGCACTCCTTT
ACATCTTGCTGCAACTCATGGACATTCTTTCACTTTACAAATAATGCTCC
GAAGTGGAGTGGATCCCAGTGTGACTGATAAGAGAGAATGGAGACCTGTG
CATTATGCAGCTTTTCATGGGCGGCTTGGCTGCTTGCAACTTCTTGTTAA
ATGGGGTTGTAGCATAGAAGATGTGGACTACAATGGAAACCTTCCAGTTC
ACTTAGCAGCCATGGAAGGCCACCTTCACTGTTTCAAATTCCTAGTCAGT
AGAATGAGCAGTGCGACGCAAGTTTTAAAAGCTTTCAATGATAATGGAGA
AAATGTACTGGATTTGGCCCAGAGGTTCTTCAAGCAGAACATTTTACAGT
TTATCCAGGGGGCTGAGTATGAAGGAAAAGACCTAGAGGATCAGGAAACT
TTAGCATTTCCAGGTCATGTGGCTGCCTTTAAGGGTGATTTGGGGATGCT
TAAGAAATTAGTGGAAGATGGAGTAATCAATATTAATGAGCGTGCTGATA
ATGGATCAACTCCTATGCATAAAGCTGCTGGACAAGGCCACATAGAGTGT
TTGCAGTGGTTAATTAAAATGGGAGCAGACAGTAATATTACCAACAAAGC
AGGGGAGAGACCCAGTGATGTGGCAAAGAGGTTTGCCCATTTGGCAGCAG
TGAAGCTGTTAGAGGAGCTACAGAAATATGATATAGATGACGAAAATGAA
ATTGATGAAAATGATGTGAAATATTTTATAAGACATGGTGTTGAGGGAAG
CACTGATGCCAAGGATGATTTATGTCTGAGTGACTTGGATAAAACAGATG
CCAGAATGAGAGCTTACAAGAAAATTGTAGAATTGAGACACCTCCTGGAA
ATTGCCGAGAGCAACTATAAACACTTGGGAGGCATAACAGAAGAAGATTT
AAAGCAGAAGAAAGAACAGCTTGAGTCTGAAAAGACCATCAAAGAACTGC
AGGGCCAGCTGGAGTATGAACGACTACGTAGAGAAAAATTAGAATGTCAG
CTTGATGAATATCGAGCAGAAGTTGATCAACTCAGGGAAACACTGGAAAA
AATTCAAGTCCCAAACTTTGTGGCTATGACAGCGCTTCTTGTGAGTCAAA
CAAAGAGAAGAGGCGAGTAAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAADRGY
AASDSSGVSGSEDASYRFSGFWERESEDEGFRCSFWERAREDLGPRPSDD
GEEGRCRCSGSWVRASEDRRSIRGLDSTPPQSRRCCAMPGVANSGPSTSS
RETANPCSRKKVHAGSIHDAVRAGDVKQLSEIVVRGASINELDVLHKFTP
LHWAAHSGSLECLHWLLWHGADITHVTTRGWTASHIAAIRGQDACVQALI
MNGANLTAQDDRGCTPLHLAATHGHSFTLQIMLRSGVDPSVTDKREWRPV
HYAAFHGRLGCLQLLVKWGCSIEDVDYNGNLPVHLAAMEGHLHCFKFLVS
RMSSATQVLKAFNDNGENVLDLAQRFFKQNILQFIQGAEYEGKDLEDQET
LAFPGHVAAFKGDLGMLKKLVEDGVININERADNGSTPMHKAAGQGHIEC
LQWLIKMGADSNITNKAGERPSDVAKRFAHLAAVKLLEELQKYDIDDENE
IDENDVKYFIRHGVEGSTDAKDDLCLSDLDKTDARMRAYKKIVELRHLLE
IAESNYKHLGGITEEDLKQKKEQLESEKTIKELQGQLEYERLRREKLECQ
LDEYRAEVDQLRETLEKIQVPNFVAMTALLVSQTKRRGE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4739
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.