cDNA Clone
WNK3

Plasmid
pEBG6P-1 WNK3 1-579 (destroyed BamHI to allow BN cloning)
Parent Plasmid
pEBG6P
Expressed
GST-WNK3 1-579
Genbank
NM_020922
Tag
GST
Species
Human
Sequence of Insert
ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGGCCACTGATTCAGGCGATCCAGCCAGCACAGAAGAT
TCTGAGAAACCTGATGGAATTTCATTTGAAAACAGAGTTCCCCAGGTCGC
TGCAACTTTGACAGTAGAAGCTAGACTAAAGGAGAAAAACAGTACCTTCT
CTGCTTCTGGGGAAACTGTAGAAAGGAAGAGATTTTTCCGAAAGAGTGTT
GAAATGACGGAAGATGACAAAGTTGCCGAATCATCCCCCAAAGATGAGAG
AATTAAGGCCGCAATGAATATTCCAAGAGTAGATAAGCTTCCTTCAAATG
TGTTGAGAGGTGGACAAGAAGTTAAATATGAACAGTGTTCAAAGTCAACC
TCAGAAATCTCAAAAGATTGTTTCAAGGAGAAAAATGAAAAGGAAATGGA
AGAAGAAGCAGAAATGAAGGCTGTAGCTACTTCTCCTAGTGGCAGATTCC
TGAAATTTGACATAGAACTAGGAAGAGGAGCATTTAAAACAGTATATAAA
GGACTGGACACTGAAACATGGGTTGAGGTTGCTTGGTGTGAGCTGCAGGA
CCGAAAGTTAACCAAAGCTGAGCAGCAAAGATTCAAGGAAGAAGCAGAGA
TGTTGAAGGGTCTCCAGCACCCCAATATAGTTCGATTTTATGATTCCTGG
GAATCTATATTAAAAGGAAAGAAATGTATTGTATTAGTGACTGAACTAAT
GACATCTGGGACCTTAAAGACGTACTTAAAACGATTTAAAGTCATGAAAC
CAAAGGTCTTAAGGAGCTGGTGCAGGCAAATTTTAAAGGGGTTGCAGTTC
TTGCACACTAGGACTCCTCCTATTATTCACCGGGACCTGAAGTGTGACAA
TATTTTCATCACGGGACCCACTGGATCTGTGAAGATTGGTGATCTAGGAT
TAGCCACCTTAATGCGTACCTCATTTGCTAAGAGTGTCATTGGAACTCCT
GAGTTTATGGCTCCAGAGATGTATGAAGAACACTATGATGAATCCGTAGA
TGTTTATGCTTTTGGAATGTGTATGCTGGAAATGGCCACATCGGAGTATC
CTTATTCTGAGTGTCAGAATGCAGCTCAAATATACCGGAAAGTAACTAGT
GGCATAAAACCAGCCAGCTTCAATAAAGTCACTGATCCTGAAGTCAAGGA
AATCATTGAAGGATGTATTCGTCAAAACAAATCTGAAAGGTTGTCTATCA
GGGACCTATTAAACCATGCATTTTTTGCTGAGGATACAGGACTGAGGGTG
GAGTTAGCAGAAGAAGATGATTGCTCAAATTCATCCCTTGCTTTAAGACT
CTGGGTTGAAGACCCTAAAAAATTGAAAGGCAAACACAAAGACAATGAAG
CTATTGAATTTAGTTTCAACCTAGAAACAGATACACCTGAGGAAGTAGCA
TATGAAATGGTCAAGTCTGGGTTCTTCCATGAAAGTGATTCCAAAGCTGT
TGCTAAATCCATTAGAGACCGGGTGACGCCAATAAAGAAGACAAGAGAGA
AGAAGCCTGCTGGCTGTTTGGAAGAACGCAGGGATTCTCAGTGCAAGTCT
ATGGGGAATGTATTCCCTCAGCCCCAGAATACAACTTTACCCCTTGCTCC
CGCTCAGCAAACTGGGGCTGAATGTGAAGAAACTGAAGTTGATCAACATG
TTAGACAACAGCTTCTACAAAGAAAACCACAGCAGCACTGCTCCTCTGTT
ACAGGTGACAATTTGTCTGAGGCAGGAGCTGCATCAGTTATACATTCATG
AGCGGCCGCG
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMATDSGDPASTED
SEKPDGISFENRVPQVAATLTVEARLKEKNSTFSASGETVERKRFFRKSV
EMTEDDKVAESSPKDERIKAAMNIPRVDKLPSNVLRGGQEVKYEQCSKST
SEISKDCFKEKNEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTVYK
GLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSW
ESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQF
LHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTP
EFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTS
GIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIRDLLNHAFFAEDTGLRV
ELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLETDTPEEVA
YEMVKSGFFHESDSKAVAKSIRDRVTPIKKTREKKPAGCLEERRDSQCKS
MGNVFPQPQNTTLPLAPAQQTGAECEETEVDQHVRQQLLQRKPQQHCSSV
TGDNLSEAGAASVIHS*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU4633
Location
PPU
MTA
Notes
Mammalian expression with N term GST.

All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.
Price
£125.00
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