cDNA Clone PfSUB-1

• Plasmid: pEBG6P-1 PfSUB-1
• Parent Plasmid: pEBG6P
• Expressed: GST-PfSUB-1
• Genbank: AJ242589
• Tag: GST
• Species: Plasmodium falciparum

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGATGCTGAACAAGAAGGTGGTGGCCTTGTGTACTTTG
ACTTTGCACTTGTTCTGTATCTTCCTGTGTTTGGGTAAGGAGGTACGTAG
CGAGGAGAACGGAAAGATCCAAGACGACGCTAAGAAGATCGTGTCTGAGC
TGAGATTCTTGGAGAAGGTTGAAGACGTGATCGAGAAGTCCAACATCGGA
GGTAACGAAGTTGACGCTGACGAGAACTCGTTCAACCCGGACACTGAAGT
CCCAATCGAAGAGATTGAGGAGATCAAGATGAGAGAGCTGAAGGACGTCA
AGGAGGAGAAGAACAAGAACGATAACCACAACAACAACAATAACAACAAC
AACATCTCGTCTTCCTCAAGTTCCTCTTCTAACACTTTCGGAGAGGAGAA
GGAGGAGGTCTCGAAGAAGAAGAAGAAGCTGAGATTGATCGTGTCTGAGA
ACCATGCCACTACTCCATCTTTCTTCCAAGAGTCTCTGTTGGAGCCCGAC
GTGCTGTCCTTCTTGGAGTCTAAGGGAAACTTGTCTAACCTGAAGAACAT
CAACTCCATGATCATCGAGCTGAAGGAGGATACCACTGATGATGAGTTGA
TCTCGTACATCAAGATCTTGGAAGAGAAGGGTGCTTTGATCGAGTCTGAC
AAGTTGGTGTCCGCTGATAACATCGACATCTCTGGTATCAAGGATGCCAT
CAGACGTGGAGAGGAGAACATCGATGTCAACGATTACAAGTCCATGCTGG
AGGTCGAGAACGATGCTGAGGACTACGACAAGATGTTCGGAATGTTCAAC
GAGTCTCACGCAGCTACTTCTAAGCGCAAGAGACACTCCACTAACGAGAG
AGGATACGATACCTTCTCCAGCCCAAGCTACAAGACTTACTCCAAGTCTG
ATTACTTGTACGATGATGACAACAATAATAACAACTACTACTACTCCCAT
TCGTCTAACGGCCATAACTCCTCCTCTCGTAACTCGTCTTCCTCTAGATC
CAGACCCGGTAAGTACCACTTCAACGATGAGTTCAGAAACCTGCAATGGG
GTCTGGACCTGTCTCGTTTGGACGAGACCCAAGAGTTGATCAACGAGCAC
CAGGTCATGAGTACCAGAATCTGTGTCATCGACTCTGGCATCGACTACAA
CCATCCAGATTTGAAGGACAACATCGAGTTGAACCTGAAGGAGTTGCACG
GTCGTAAGGGTTTCGATGACGATAACAACGGCATCGTGGACGACATCTAC
GGTGCTAACTTCGTGAACAACTCTGGCAACCCCATGGATGATAACTACCA
CGGAACTCATGTCTCTGGAATCATCTCCGCTATCGGAAACAATAACATCG
GAGTGGTCGGAGTGGATGTCAACTCCAAGTTGATCATCTGTAAGGCCCTG
GATGAGCATAAGCTGGGAAGATTGGGAGATATGTTCAAGTGTTTGGACTA
CTGTATCTCGCGTAACGCTCATATGATCAACGGTTCCTTCTCTTTCGACG
AATACTCCGGTATCTTCAACTCTTCTGTCGAGTACCTGCAAAGAAAGGGT
ATCTTGTTCTTCGTGTCTGCGTCGAACTGTTCCCATCCAAAGTCTAGCAC
CCCAGATATCCGCAAGTGTGACCTGTCTATCAACGCTAAGTACCCACCCA
TCTTGTCTACTGTCTACGACAACGTTATCTCTGTGGCTAACTTGAAGAAG
AACGATAATAACAACCACTACTCTCTGTCGATCAACTCTTTCTACTCCAA
CAAGTACTGTCAATTGGCAGCTCCAGGTACTAACATCTACTCGACCGCTC
CACATAACTCTTACAGAAAGTTGAACGGTACCTCTATGGCTGCTCCACAT
GTTGCTGCCATCGCTAGCTTGATCTTCTCAATCAACCCAGACCTGTCCTA
CAAGAAGGTCATCCAGATCCTTAAGGATTCCATCGTGTACTTGCCATCCT
TGAAGAACATGGTTGCTTGGGCTGGTTACGCTGACATCAACAAGGCTGTC
AACTTGGCTATCAAGTCCAAGAAGACGTACATCAACTCTAACATCTCCAA
CAAGTGGAAGAAGAAGTCCAGATACCTGCACCACCACCATCACCATTAGG
CGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMMLNKKVVALCTL
TLHLFCIFLCLGKEVRSEENGKIQDDAKKIVSELRFLEKVEDVIEKSNIG
GNEVDADENSFNPDTEVPIEEIEEIKMRELKDVKEEKNKNDNHNNNNNNN
NISSSSSSSSNTFGEEKEEVSKKKKKLRLIVSENHATTPSFFQESLLEPD
VLSFLESKGNLSNLKNINSMIIELKEDTTDDELISYIKILEEKGALIESD
KLVSADNIDISGIKDAIRRGEENIDVNDYKSMLEVENDAEDYDKMFGMFN
ESHAATSKRKRHSTNERGYDTFSSPSYKTYSKSDYLYDDDNNNNNYYYSH
SSNGHNSSSRNSSSSRSRPGKYHFNDEFRNLQWGLDLSRLDETQELINEH
QVMSTRICVIDSGIDYNHPDLKDNIELNLKELHGRKGFDDDNNGIVDDIY
GANFVNNSGNPMDDNYHGTHVSGIISAIGNNNIGVVGVDVNSKLIICKAL
DEHKLGRLGDMFKCLDYCISRNAHMINGSFSFDEYSGIFNSSVEYLQRKG
ILFFVSASNCSHPKSSTPDIRKCDLSINAKYPPILSTVYDNVISVANLKK
NDNNNHYSLSINSFYSNKYCQLAAPGTNIYSTAPHNSYRKLNGTSMAAPH
VAAIASLIFSINPDLSYKKVIQILKDSIVYLPSLKNMVAWAGYADINKAV
NLAIKSKKTYINSNISNKWKKKSRYLHHHHHH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4577
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST tag.