cDNA Clone PfSUB-1

• Plasmid: pCMV5-HA-1 PfSUB-1
• Parent Plasmid: pCMV5
• Expressed: HA-PfSUB-1
• Genbank: AJ242589
• Tag: HA
• Species: Plasmodium falciparum

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCATGATGCT
GAACAAGAAGGTGGTGGCCTTGTGTACTTTGACTTTGCACTTGTTCTGTA
TCTTCCTGTGTTTGGGTAAGGAGGTACGTAGCGAGGAGAACGGAAAGATC
CAAGACGACGCTAAGAAGATCGTGTCTGAGCTGAGATTCTTGGAGAAGGT
TGAAGACGTGATCGAGAAGTCCAACATCGGAGGTAACGAAGTTGACGCTG
ACGAGAACTCGTTCAACCCGGACACTGAAGTCCCAATCGAAGAGATTGAG
GAGATCAAGATGAGAGAGCTGAAGGACGTCAAGGAGGAGAAGAACAAGAA
CGATAACCACAACAACAACAATAACAACAACAACATCTCGTCTTCCTCAA
GTTCCTCTTCTAACACTTTCGGAGAGGAGAAGGAGGAGGTCTCGAAGAAG
AAGAAGAAGCTGAGATTGATCGTGTCTGAGAACCATGCCACTACTCCATC
TTTCTTCCAAGAGTCTCTGTTGGAGCCCGACGTGCTGTCCTTCTTGGAGT
CTAAGGGAAACTTGTCTAACCTGAAGAACATCAACTCCATGATCATCGAG
CTGAAGGAGGATACCACTGATGATGAGTTGATCTCGTACATCAAGATCTT
GGAAGAGAAGGGTGCTTTGATCGAGTCTGACAAGTTGGTGTCCGCTGATA
ACATCGACATCTCTGGTATCAAGGATGCCATCAGACGTGGAGAGGAGAAC
ATCGATGTCAACGATTACAAGTCCATGCTGGAGGTCGAGAACGATGCTGA
GGACTACGACAAGATGTTCGGAATGTTCAACGAGTCTCACGCAGCTACTT
CTAAGCGCAAGAGACACTCCACTAACGAGAGAGGATACGATACCTTCTCC
AGCCCAAGCTACAAGACTTACTCCAAGTCTGATTACTTGTACGATGATGA
CAACAATAATAACAACTACTACTACTCCCATTCGTCTAACGGCCATAACT
CCTCCTCTCGTAACTCGTCTTCCTCTAGATCCAGACCCGGTAAGTACCAC
TTCAACGATGAGTTCAGAAACCTGCAATGGGGTCTGGACCTGTCTCGTTT
GGACGAGACCCAAGAGTTGATCAACGAGCACCAGGTCATGAGTACCAGAA
TCTGTGTCATCGACTCTGGCATCGACTACAACCATCCAGATTTGAAGGAC
AACATCGAGTTGAACCTGAAGGAGTTGCACGGTCGTAAGGGTTTCGATGA
CGATAACAACGGCATCGTGGACGACATCTACGGTGCTAACTTCGTGAACA
ACTCTGGCAACCCCATGGATGATAACTACCACGGAACTCATGTCTCTGGA
ATCATCTCCGCTATCGGAAACAATAACATCGGAGTGGTCGGAGTGGATGT
CAACTCCAAGTTGATCATCTGTAAGGCCCTGGATGAGCATAAGCTGGGAA
GATTGGGAGATATGTTCAAGTGTTTGGACTACTGTATCTCGCGTAACGCT
CATATGATCAACGGTTCCTTCTCTTTCGACGAATACTCCGGTATCTTCAA
CTCTTCTGTCGAGTACCTGCAAAGAAAGGGTATCTTGTTCTTCGTGTCTG
CGTCGAACTGTTCCCATCCAAAGTCTAGCACCCCAGATATCCGCAAGTGT
GACCTGTCTATCAACGCTAAGTACCCACCCATCTTGTCTACTGTCTACGA
CAACGTTATCTCTGTGGCTAACTTGAAGAAGAACGATAATAACAACCACT
ACTCTCTGTCGATCAACTCTTTCTACTCCAACAAGTACTGTCAATTGGCA
GCTCCAGGTACTAACATCTACTCGACCGCTCCACATAACTCTTACAGAAA
GTTGAACGGTACCTCTATGGCTGCTCCACATGTTGCTGCCATCGCTAGCT
TGATCTTCTCAATCAACCCAGACCTGTCCTACAAGAAGGTCATCCAGATC
CTTAAGGATTCCATCGTGTACTTGCCATCCTTGAAGAACATGGTTGCTTG
GGCTGGTTACGCTGACATCAACAAGGCTGTCAACTTGGCTATCAAGTCCA
AGAAGACGTACATCAACTCTAACATCTCCAACAAGTGGAAGAAGAAGTCC
AGATACCTGCACCACCACCATCACCATTAGGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSMMLNKKVVALCTLTLHLFCIFLCLGKEVRSEENGKIQD
DAKKIVSELRFLEKVEDVIEKSNIGGNEVDADENSFNPDTEVPIEEIEEI
KMRELKDVKEEKNKNDNHNNNNNNNNISSSSSSSSNTFGEEKEEVSKKKK
KLRLIVSENHATTPSFFQESLLEPDVLSFLESKGNLSNLKNINSMIIELK
EDTTDDELISYIKILEEKGALIESDKLVSADNIDISGIKDAIRRGEENID
VNDYKSMLEVENDAEDYDKMFGMFNESHAATSKRKRHSTNERGYDTFSSP
SYKTYSKSDYLYDDDNNNNNYYYSHSSNGHNSSSRNSSSSRSRPGKYHFN
DEFRNLQWGLDLSRLDETQELINEHQVMSTRICVIDSGIDYNHPDLKDNI
ELNLKELHGRKGFDDDNNGIVDDIYGANFVNNSGNPMDDNYHGTHVSGII
SAIGNNNIGVVGVDVNSKLIICKALDEHKLGRLGDMFKCLDYCISRNAHM
INGSFSFDEYSGIFNSSVEYLQRKGILFFVSASNCSHPKSSTPDIRKCDL
SINAKYPPILSTVYDNVISVANLKKNDNNNHYSLSINSFYSNKYCQLAAP
GTNIYSTAPHNSYRKLNGTSMAAPHVAAIASLIFSINPDLSYKKVIQILK
DSIVYLPSLKNMVAWAGYADINKAVNLAIKSKKTYINSNISNKWKKKSRY
LHHHHHH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4576
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term HA tag.