cDNA Clone PPP2R5B

• Plasmid: pEBG6P-1 PPP2R5B
• Parent Plasmid: pEBG6P
• Expressed: GST-PPP2R5B
• Genbank: BC045619
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCATCGATGCGGCCGCGATGGAGACGAAG
CTGCCCCCTGCAAGCACCCCCACTAGCCCCTCCTCCCCCGGGCTGTCGCC
TGTGCCCCCACCCGACAAGGTGGACGGCTTCTCCCGCCGTTCCCTCCGCA
GAGCCCGGCCCCGCCGCTCCCACAGCTCCTCTCAGTTCCGCTATCAGAGC
AACCAGCAAGAGCTCACACCGCTGCCCCTGCTCAAAGATGTGCCGGCTTC
CGAGCTGCACGAGCTGCTGAGCCGGAAGCTGGCCCAGTGTGGGGTGATGT
TTGACTTCTTGGACTGTGTGGCCGACCTCAAGGGGAAGGAGGTGAAGCGG
GCAGCCCTCAACGAGCTGGTGGAGTGTGTGGGGAGCACCCGGGGTGTCCT
CATCGAGCCCGTCTACCCAGACATCATCCGCATGATCTCAGTGAATATCT
TCCGGACTCTGCCGCCCAGTGAGAACCCTGAATTTGACCCTGAAGAGGAT
GAGCCCAATCTTGAGCCTTCGTGGCCACACCTGCAGCTGGTATATGAGTT
TTTCTTGCGTTTCTTGGAGAGCCCAGACTTCCAGCCCTCCGTGGCCAAGA
GATATGTGGATCAAAAGTTTGTCCTGATGCTCCTGGAGCTATTTGATAGT
GAGGATCCCCGGGAGCGTGAGTACCTCAAGACCATCCTGCACCGGGTCTA
TGGCAAGTTCCTGGGTCTCCGGGCCTACATCCGCAAACAGTGCAACCACA
TCTTCCTCCGGTTCATCTATGAATTCGAGCACTTCAATGGTGTGGCTGAG
CTGCTGGAGATCCTAGGAAGCATCATCAATGGCTTTGCGCTGCCCCTGAA
GACGGAGCACAAGCAGTTCCTGGTTCGCGTCCTGATCCCCCTGCACTCTG
TCAAGTCGCTGTCTGTCTTCCATGCCCAGCTGGCATACTGTGTGGTGCAG
TTCCTGGAGAAGGATGCCACTCTGACAGAGCACGTGATCCGGGGGCTGCT
CAAATACTGGCCAAAAACCTGCACCCAGAAGGAGGTGATGTTTCTGGGGG
AGATGGAAGAGATTCTTGATGTCATCGAGCCCTCCCAGTTTGTGAAGATC
CAGGAGCCCCTTTTTAAGCAGGTGGCTCGCTGTGTTTCCAGCCCCCATTT
CCAGGTTGCAGAGCGGGCTCTGTATTTCTGGAACAATGAGTATATCCTAA
GCCTCATTGAGGACAACTGCCACACTGTGCTGCCTGCTGTGTTTGGGACC
CTCTACCAAGTCTCCAAGGAGCACTGGAACCAAACCATCGTATCACTGAT
CTACAATGTGCTCAAGACCTTCATGGAGATGAATGGGAAGCTGTTTGATG
AGCTCACAGCCTCCTACAAGCTGGAAAAGCAGCAGGAGCAGCAGAAGGCC
CAGGAGCGTCAGGAGTTATGGCAAGGTCTGGAGGAGCTGCGGCTACGCCG
GCTACAGGGGACCCAGGGGGCCAAGGAGGCCCCCCTCCAGCGGCTTACAC
CCCAGGTGGCCGCCAGTGGGGGTCAGAGCTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMETK
LPPASTPTSPSSPGLSPVPPPDKVDGFSRRSLRRARPRRSHSSSQFRYQS
NQQELTPLPLLKDVPASELHELLSRKLAQCGVMFDFLDCVADLKGKEVKR
AALNELVECVGSTRGVLIEPVYPDIIRMISVNIFRTLPPSENPEFDPEED
EPNLEPSWPHLQLVYEFFLRFLESPDFQPSVAKRYVDQKFVLMLLELFDS
EDPREREYLKTILHRVYGKFLGLRAYIRKQCNHIFLRFIYEFEHFNGVAE
LLEILGSIINGFALPLKTEHKQFLVRVLIPLHSVKSLSVFHAQLAYCVVQ
FLEKDATLTEHVIRGLLKYWPKTCTQKEVMFLGEMEEILDVIEPSQFVKI
QEPLFKQVARCVSSPHFQVAERALYFWNNEYILSLIEDNCHTVLPAVFGT
LYQVSKEHWNQTIVSLIYNVLKTFMEMNGKLFDELTASYKLEKQQEQQKA
QERQELWQGLEELRLRRLQGTQGAKEAPLQRLTPQVAASGGQS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4544
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.