cDNA Clone PUMH2

• Plasmid: pGEX6P-1-PUMH2 delta 574-692
• Parent Plasmid: pGEX6P1
• Expressed: GST-PUMH2 delta 574-692
• Genbank: AF315591
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAG
CTTTTGCCTACCAAAAAGTTTTGGGAACCTGATGATTCAACAAAAGATGG
ACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGAGACTGCATGGG
GAGCTTCTCACCATTCAATGTCCCAGCCTATTATGGTACAGAGAAGATCT
GGACAGGGTTTTCATGGAAACAGTGAAGTAAATGCAATACTGTCTCCGCG
ATCAGAAAGTGGAGGCCTTGGTGTGAGCATGGTAGAATATGTATTAAGTT
CTTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAAATTTTGGC
ACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAA
GGCTTCTCCATTTGAGGAGGACCAAAACAGAGATCTTAAACAAGGAGATG
ATGATGATTCTAAAATAAATGGCAGAGGTTTGCCAAATGGAATGGATGCC
GATTGCAAAGATTTTAATCGTACTCCTGGAAGTCGTCAAGCCTCTCCAAC
TGAAGTAGTTGAGCGCTTGGGCCCCAATACTAATCCCTCAGAAGGACTGG
GGCCTCTTCCTAATCCTACAGCTAATAAACCACTTGTTGAAGAATTTTCA
AATCCTGAAACTCAGAATCTGGATGCCATGGAACAAGTTGGTCTGGAATC
CTTACAGTTTGACTATCCTGGTAATCAGGTACCAATGGACTCTTCAGGAG
CTACTGTAGGCCTTTTTGACTACAATTCCCAGCAGCAGCTCTTTCAGAGG
ACTAATGCACTAACAGTTCAACAGTTAACTGCAGCTCAACAGCAGCAATA
TGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAG
GCCTTGCTCCAGCTGCATTTGTGCCAAATCCATACATTATTAGTGCTGCT
CCTCCAGGGACCGATCCGTATACTGCAGCAGGATTGGCTGCAGCAGCTAC
ATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGCGTTCCATGGG
GGGTGTATCCAGCCAACTTATTTCAGCAGCAAGCTGCAGCTGCGGCAAAT
AACACAGCCAGTCAGCAAGCAGCATCACAAGCTCAGCCTGGACAGCAACA
GGTTCTCCGTGCTGGAGCAGGTCAGCGTCCTCTTACTCCCAATCAGGGTC
AGCAAGGGCAGCAAGCAGAATCACTTGCGGCAGCTGCAGCAGCAAATCCA
ACATTGGCTTTTGGTCAGGGTCTTGCTACTGGCATGCCAGGCTATCAAGT
ACTAGCTCCAACTGCCTATTATGATCAGACTGGTGCCTTAGTGGTTGGCC
CTGGAGCAAGGACTGGCCTTGGAGCTCCAGTTCGGTTAATGGCTCCAACA
CCTGTTTTAATTAGTTCAGCAGCAGCACAAGCTGCAGCAGCAGCAGCAGC
TGGAGGAACTGCAAGTAGCCTTACAGGCAGCACAAATGGTCTGTTTCGGC
CAATTGGCACTCAGCCACCACAGCAGCAGCAACAGCAGCCAAGCACTAAT
CTGCAATCTAATTCATTTTATGGAAGCAGTTCTTTGACTAATAGCTCCCA
GAGTAGTTCTTTATTTTCTCATGGACCTGGTCAACCTGGAAGTACATCTC
TTGGCTTTGGAAGTGGTAACTCTTTGGGTGCTGCTATAGGCTCAGCCCTC
AGTGGATTTGGTTCATCAGGAGGACTGACAAATGGTAGTGGTCGATATAT
CTCTGCAGCACCTGGAGCAGAAGCAAAATATCGAAGTGCTTCAAGCACTT
CCAGTCTATTTAGCTCCAGCAGCCAGCTCTTTCCTCCTTCCCGGCTTCGG
TATAATAGGTCTGATATTATGCCTTCTGGCCGCAGTAGATTATTGGAAGA
TTTCAGAAACAACCGCTTCCCAAACCTTCAGCTTAGAGACTTGATTGGAC
ATATAGTTGAGTTTTCTCAAGACCAGCATGGTTCTAGATTCATACAGCAA
AAACTAGAGAGAGCTACTCCAGCTGAGCGACAGATGGTATTTAATGAAAT
TCTGCAAGCAGCCTATCAATTAATGACTGATGTTTTTGGCAACTATGTTA
TACAGAAGTTTTTTGAGTTTGGGAGTCTGGATCAAAAATTAGCCCTGGCT
ACTCGTATTCGTGGTCATGTTCTACCCTTAGCCTTGCAGATGTATGGCTG
CCGCGTTATTCAGAAAGCATTAGAATCTATTTCTTCTGACCAGCAGAGTG
AAATGGTAAAGGAGCTGGATGGTCATGTGCTCAAATGTGTAAAAGATCAG
AATGGAAACCATGTTGTACAAAAATGTATCGAATGTGTTCAGCCACAGTC
ACTACAGTTCATCATTGATGCTTTCAAGGGACAAGTATTTGTGCTTTCAA
CTCATCCTTATGGCTGCAGAGTAATTCAGCGCATCCTAGAGCATTGCACT
GCAGAACAGACCTTACCTATCTTAGAAGAACTCCACCAACATACAGAGCA
GTTGGTACAGGATCAGTATGGCAATTATGTTATTCAGCATGTACTGGAAC
ACGGTCGACCTGAAGACAAGAGCAAAATTGTTTCCGAAATCAGGGGAAAG
GTTTTAGCCCTGAGTCAACACAAATTTGCCAGCAATGTAGTAGAAAAGTG
TGTTACTCATGCCTCCCGTGCTGAGAGAGCTTTACTGATTGACGAGGTTT
GCTGCCAGAATGATGGTCCTCACAGTGCCTTATACACCATGATGAAGGAC
CAGTATGCCAATTACGTGGTTCAAAAGATGATTGATATGGCTGAACCTGC
TCAGAGAAAGATAATCATGCACAAGATTCGACCTCACATTACTACTTTGC
GCAAATACACATACGGGAAGCATATACTGGCCAAGTTGGAAAAGTATTAT
TTGAAGAATAGCCCGGACCTAGGACCTATTGGAGGACCACCAAATGGAAT
GCTGTAAGCGGCCGCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFNHDFQALALESRGMGE
LLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGASHHSMSQPIMVQRRS
GQGFHGNSEVNAILSPRSESGGLGVSMVEYVLSSSPADKLDSRFRKGNFG
TRDAETDGPEKGDQKGKASPFEEDQNRDLKQGDDDDSKINGRGLPNGMDA
DCKDFNRTPGSRQASPTEVVERLGPNTNPSEGLGPLPNPTANKPLVEEFS
NPETQNLDAMEQVGLESLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQR
TNALTVQQLTAAQQQQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAA
PPGTDPYTAAGLAAAATLAGPAVVPPQYYGVPWGVYPANLFQQQAAAAAN
NTASQQAASQAQPGQQQVLRAGAGQRPLTPNQGQQGQQAESLAAAAAANP
TLAFGQGLATGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPT
PVLISSAAAQAAAAAAAGGTASSLTGSTNGLFRPIGTQPPQQQQQQPSTN
LQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSTSLGFGSGNSLGAAIGSAL
SGFGSSGGLTNGSGRYISAAPGAEAKYRSASSTSSLFSSSSQLFPPSRLR
YNRSDIMPSGRSRLLEDFRNNRFPNLQLRDLIGHIVEFSQDQHGSRFIQQ
KLERATPAERQMVFNEILQAAYQLMTDVFGNYVIQKFFEFGSLDQKLALA
TRIRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVLKCVKDQ
NGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCT
AEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGK
VLALSQHKFASNVVEKCVTHASRAERALLIDEVCCQNDGPHSALYTMMKD
QYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYY
LKNSPDLGPIGGPPNGML*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4321
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.