cDNA Clone PUMH2

• Plasmid: pGEX6P-1-PUMH2 FL
• Parent Plasmid: pGEX6P1
• Expressed: GST-PUMH2 FL
• Genbank: AF315591
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAAT
TCAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAG
CTTTTGCCTACCAAAAAGTTTTGGGAACCTGATGATTCAACAAAAGATGG
ACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGAGACTGCATGGG
GAGCTTCTCACCATTCAATGTCCCAGCCTATTATGGTACAGAGAAGATCT
GGACAGGGTTTTCATGGAAACAGTGAAGTAAATGCAATACTGTCTCCGCG
ATCAGAAAGTGGAGGCCTTGGTGTGAGCATGGTAGAATATGTATTAAGTT
CTTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAAATTTTGGC
ACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAA
GGCTTCTCCATTTGAGGAGGACCAAAACAGAGATCTTAAACAAGGAGATG
ATGATGATTCTAAAATAAATGGCAGAGGTTTGCCAAATGGAATGGATGCC
GATTGCAAAGATTTTAATCGTACTCCTGGAAGTCGTCAAGCCTCTCCAAC
TGAAGTAGTTGAGCGCTTGGGCCCCAATACTAATCCCTCAGAAGGACTGG
GGCCTCTTCCTAATCCTACAGCTAATAAACCACTTGTTGAAGAATTTTCA
AATCCTGAAACTCAGAATCTGGATGCCATGGAACAAGTTGGTCTGGAATC
CTTACAGTTTGACTATCCTGGTAATCAGGTACCAATGGACTCTTCAGGAG
CTACTGTAGGCCTTTTTGACTACAATTCCCAGCAGCAGCTCTTTCAGAGG
ACTAATGCACTAACAGTTCAACAGTTAACTGCAGCTCAACAGCAGCAATA
TGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAG
GCCTTGCTCCAGCTGCATTTGTGCCAAATCCATACATTATTAGTGCTGCT
CCTCCAGGGACCGATCCGTATACTGCAGCAGGATTGGCTGCAGCAGCTAC
ATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGCGTTCCATGGG
GGGTGTATCCAGCCAACTTATTTCAGCAGCAAGCTGCAGCTGCGGCAAAT
AACACAGCCAGTCAGCAAGCAGCATCACAAGCTCAGCCTGGACAGCAACA
GGTTCTCCGTGCTGGAGCAGGTCAGCGTCCTCTTACTCCCAATCAGGGTC
AGCAAGGGCAGCAAGCAGAATCACTTGCGGCAGCTGCAGCAGCAAATCCA
ACATTGGCTTTTGGTCAGGGTCTTGCTACTGGCATGCCAGGCTATCAAGT
ACTAGCTCCAACTGCCTATTATGATCAGACTGGTGCCTTAGTGGTTGGCC
CTGGAGCAAGGACTGGCCTTGGAGCTCCAGTTCGGTTAATGGCTCCAACA
CCTGTTTTAATTAGTTCAGCAGCAGCACAAGCTGCAGCAGCAGCAGCAGC
TGGAGGAACTGCAAGTAGCCTTACAGGCAGCACAAATGGTCTGTTTCGGC
CAATTGGCACTCAGCCACCACAGCAGCAGCAACAGCAGCCAAGCACTAAT
CTGCAATCTAATTCATTTTATGGAAGCAGTTCTTTGACTAATAGCTCCCA
GAGTAGTTCTTTATTTTCTCATGGACCTGGTCAACCTGGAAGTACATCTC
TTGGCTTTGGAAGTGGTAACTCTTTGGGTGCTGCTATAGGCTCAGCCCTC
AGTGGATTTGGTTCATCAGTTGGCAGTTCTGCAAGTAGTAGTGCCACAAG
GAGAGAGTCTCTATCTACTAGCTCTGACTTGTACAAAAGATCTAGTAGCA
GCCTAGCACCCATAGGGCAACCATTTTACAATAGTCTGGGATTTTCCTCC
TCTCCAAGTCCAATAGGCATGCCTCTGCCAAGCCAAACTCCAGGACATTC
ACTTACGCCACCGCCATCACTTTCATCACATGGATCCTCATCCAGTTTGC
ATTTAGGAGGACTGACAAATGGTAGTGGTCGATATATCTCTGCAGCACCT
GGAGCAGAAGCAAAATATCGAAGTGCTTCAAGCACTTCCAGTCTATTTAG
CTCCAGCAGCCAGCTCTTTCCTCCTTCCCGGCTTCGGTATAATAGGTCTG
ATATTATGCCTTCTGGCCGCAGTAGATTATTGGAAGATTTCAGAAACAAC
CGCTTCCCAAACCTTCAGCTTAGAGACTTGATTGGACATATAGTTGAGTT
TTCTCAAGACCAGCATGGTTCTAGATTCATACAGCAAAAACTAGAGAGAG
CTACTCCAGCTGAGCGACAGATGGTATTTAATGAAATTCTGCAAGCAGCC
TATCAATTAATGACTGATGTTTTTGGCAACTATGTTATACAGAAGTTTTT
TGAGTTTGGGAGTCTGGATCAAAAATTAGCCCTGGCTACTCGTATTCGTG
GTCATGTTCTACCCTTAGCCTTGCAGATGTATGGCTGCCGCGTTATTCAG
AAAGCATTAGAATCTATTTCTTCTGACCAGCAGAGTGAAATGGTAAAGGA
GCTGGATGGTCATGTGCTCAAATGTGTAAAAGATCAGAATGGAAACCATG
TTGTACAAAAATGTATCGAATGTGTTCAGCCACAGTCACTACAGTTCATC
ATTGATGCTTTCAAGGGACAAGTATTTGTGCTTTCAACTCATCCTTATGG
CTGCAGAGTAATTCAGCGCATCCTAGAGCATTGCACTGCAGAACAGACCT
TACCTATCTTAGAAGAACTCCACCAACATACAGAGCAGTTGGTACAGGAT
CAGTATGGCAATTATGTTATTCAGCATGTACTGGAACACGGTCGACCTGA
AGACAAGAGCAAAATTGTTTCCGAAATCAGGGGAAAGGTTTTAGCCCTGA
GTCAACACAAATTTGCCAGCAATGTAGTAGAAAAGTGTGTTACTCATGCC
TCCCGTGCTGAGAGAGCTTTACTGATTGACGAGGTTTGCTGCCAGAATGA
TGGTCCTCACAGTGCCTTATACACCATGATGAAGGACCAGTATGCCAATT
ACGTGGTTCAAAAGATGATTGATATGGCTGAACCTGCTCAGAGAAAGATA
ATCATGCACAAGATTCGACCTCACATTACTACTTTGCGCAAATACACATA
CGGGAAGCATATACTGGCCAAGTTGGAAAAGTATTATTTGAAGAATAGCC
CGGACCTAGGACCTATTGGAGGACCACCAAATGGAATGCTGTAAGCGGCC
GC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFNHDFQALALESRGMGE
LLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGASHHSMSQPIMVQRRS
GQGFHGNSEVNAILSPRSESGGLGVSMVEYVLSSSPADKLDSRFRKGNFG
TRDAETDGPEKGDQKGKASPFEEDQNRDLKQGDDDDSKINGRGLPNGMDA
DCKDFNRTPGSRQASPTEVVERLGPNTNPSEGLGPLPNPTANKPLVEEFS
NPETQNLDAMEQVGLESLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQR
TNALTVQQLTAAQQQQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAA
PPGTDPYTAAGLAAAATLAGPAVVPPQYYGVPWGVYPANLFQQQAAAAAN
NTASQQAASQAQPGQQQVLRAGAGQRPLTPNQGQQGQQAESLAAAAAANP
TLAFGQGLATGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPT
PVLISSAAAQAAAAAAAGGTASSLTGSTNGLFRPIGTQPPQQQQQQPSTN
LQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSTSLGFGSGNSLGAAIGSAL
SGFGSSVGSSASSSATRRESLSTSSDLYKRSSSSLAPIGQPFYNSLGFSS
SPSPIGMPLPSQTPGHSLTPPPSLSSHGSSSSLHLGGLTNGSGRYISAAP
GAEAKYRSASSTSSLFSSSSQLFPPSRLRYNRSDIMPSGRSRLLEDFRNN
RFPNLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQMVFNEILQAA
YQLMTDVFGNYVIQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQ
KALESISSDQQSEMVKELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFI
IDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQD
QYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHA
SRAERALLIDEVCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKI
IMHKIRPHITTLRKYTYGKHILAKLEKYYLKNSPDLGPIGGPPNGML*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4320
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.