cDNA Clone PFKFB3

• Synonyms: FLJ37326, IPFK2, PFK2
• Plasmid: pGEX6P-3-PFKFB3
• Parent Plasmid: pGEX6P3
• Expressed: GST-PFKFB3
• Genbank: NM_004566.3 GI:223941849
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCGTTGGAACTGACGCAGAGCCGAGTGCAGAAGATCTGGGT
GCCCGTGGACCACAGGCCCTCGTTGCCCAGATCCTGTGGGCCAAAGCTGA
CCAACTCCCCCACCGTCATCGTCATGGTGGGCCTCCCCGCCCGGGGCAAG
ACCTACATCTCCAAGAAGCTGACTCGCTACCTCAACTGGATTGGCGTCCC
CACAAAAGTGTTCAACGTCGGGGAGTATCGCCGGGAGGCTGTGAAGCAGT
ACAGCTCCTACAACTTCTTCCGCCCCGACAATGAGGAAGCCATGAAAGTC
CGGAAGCAATGTGCCTTAGCTGCCTTGAGAGATGTCAAAAGCTACCTGGC
GAAAGAAGGGGGACAAATTGCGGTTTTCGATGCCACCAATACTACTAGAG
AGAGGAGACACATGATCCTTCATTTTGCCAAAGAAAATGACTTTAAGGCG
TTTTTCATCGAGTCGGTGTGCGACGACCCTACAGTTGTGGCCTCCAATAT
CATGGAAGTTAAAATCTCCAGCCCGGATTACAAAGACTGCAACTCGGCAG
AAGCCATGGACGACTTCATGAAGAGGATCAGTTGCTATGAAGCCAGCTAC
CAGCCCCTCGACCCCGACAAATGCGACAGGGACTTGTCGCTGATCAAGGT
GATTGACGTGGGCCGGAGGTTCCTGGTGAACCGGGTGCAGGACCACATCC
AGAGCCGCATCGTGTACTACCTGATGAACATCCACGTGCAGCCGCGTACC
ATCTACCTGTGCCGGCACGGCGAGAACGAGCACAACCTCCAGGGCCGCAT
CGGGGGCGACTCAGGCCTGTCCAGCCGGGGCAAGAAGTTTGCCAGTGCTC
TGAGCAAGTTCGTGGAGGAGCAGAACCTGAAGGACCTGCGCGTGTGGACC
AGCCAGCTGAAGAGCACCATCCAGACGGCCGAGGCGCTGCGGCTGCCCTA
CGAGCAGTGGAAGGCGCTCAATGAGATCGACGCGGGCGTCTGTGAGGAGC
TGACCTACGAGGAGATCAGGGACACCTACCCTGAGGAGTATGCGCTGCGG
GAGCAGGACAAGTACTATTACCGCTACCCCACCGGGGAGTCCTACCAGGA
CCTGGTCCAGCGCTTGGAGCCAGTGATCATGGAGCTGGAGCGGCAGGAGA
ATGTGCTGGTCATCTGCCACCAGGCCGTCCTGCGCTGCCTGCTTGCCTAC
TTCCTGGATAAGAGTGCAGAGGAGATGCCCTACCTGAAATGCCCTCTTCA
CACCGTCCTGAAACTGACGCCTGTCGCTTATGGCTGCCGTGTGGAATCCA
TCTACCTGAACGTGGAGTCCGTCTGCACACACCGGGAGAGGTCAGAGGAT
GCAAAGAAGGGACCTAACCCGCTCATGAGACGCAATAGTGTCACCCCGCT
AGCCAGCCCCGAACCCACCAAAAAGCCTCGCATCAACAGCTTTGAGGAGC
ATGTGGCCTCCACCTCGGCCGCCCTGCCCAGCTGCCTGCCCCCGGAGGTG
CCCACGCAGCTGCCTGGACAAAACATGAAAGGCTCCCGGAGCAGCGCTGA
CTCCTCCAGGAAACACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPLELTQSRVQKIWVPVDH
RPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVF
NVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSYLAKEGG
QIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVK
ISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVG
RRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDS
GLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWK
ALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQR
LEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLK
LTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPE
PTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRK
H*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36863
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 86430 Daltons
• Low Temp: No