cDNA Clone 14-3-3 epsilon

• Synonyms: YWHAE
• Plasmid: pcDNA5-FRT/TO NRL-14-3-3 epsilon
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: NRL (Rluc M1-G229) + spacer + 14-3-3 epsilon

Sequence of Insert

GGATCCATGGATGATCGAGAGGATCTGGTGTACCAGGCGAAGCTGGCCGA
GCAGGCTGAGCGATACGACGAAATGGTGGAGTCAATGAAGAAAGTAGCAG
GGATGGATGTGGAGCTGACAGTTGAAGAAAGAAACCTCCTATCTGTTGCA
TATAAGAATGTGATTGGAGCTAGAAGAGCCTCCTGGAGAATAATCAGCAG
CATTGAACAGAAAGAAGAAAACAAGGGAGGAGAAGACAAGCTAAAAATGA
TTCGGGAATATCGGCAAATGGTTGAGACTGAGCTAAAGTTAATCTGTTGT
GACATTCTGGATGTACTGGACAAACACCTCATTCCAGCAGCTAACACTGG
CGAGTCCAAGGTTTTCTATTATAAAATGAAAGGGGACTACCACAGGTATC
TGGCAGAATTTGCCACAGGAAACGACAGGAAGGAGGCTGCGGAGAACAGC
CTAGTGGCTTATAAAGCTGCTAGTGATATTGCAATGACAGAACTTCCACC
AACGCATCCTATTCGCTTAGGTCTTGCTCTCAATTTTTCCGTATTCTACT
ACGAAATTCTTAATTCCCCTGACCGTGCCTGCAGGTTGGCAAAAGCAGCT
TTTGATGATGCAATTGCAGAACTGGATACGCTGAGTGAAGAAAGCTATAA
GGACTCTACACTTATCATGCAGTTGTTACGTGATAATCTGACACTATGGA
CTTCAGACATGCAGGGTGACGGTGAAGAGCAGAATAAAGAAGCGCTGCAG
GACGTGGAAGACGAAAATCAGTGAGCGGCCGC

Amino Acid Sequence

MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFL
HGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKY
LTAWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVI
ESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFA
AYLEPFKEKGEVRRPTLSWPREIPLVKGGGGGGSGGGGSMDDREDLVYQA
KLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWR
IISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPA
ANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMT
ELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSE
ESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36532
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: pcDNA5 FRT/TO Renilla luciferase M1-G229 + spacer DU36511