cDNA Clone TEC

• Synonyms: MGC126760, MGC126762, PSCTK4
• Plasmid: pFastbac.HTb TEC
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-TEC
• Genbank: NM_003215.2 GI:130977758
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTC
ACAGCAGAAAAAGAAGACATCGCCCTTAAACTACAAAGAGAGACTTTTTG
TACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAAGAAA
TACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGT
GAAGAATGATGATGGTGTCATTCCCTGTCAAAATAAGTATCCATTTCAGG
TTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGTCCACAAAGC
AGGGACCTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAA
TATTATGATTAAATATCATCCTAAATTCTGGACAGATGGAAGTTATCAGT
GTTGTAGACAAACTGAAAAATTAGCACCCGGATGTGAAAAATACAATCTT
TTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCACCAGAAACAAAGAA
GCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGTGAAG
AAATCGTTGTAGCCATGTATGATTTCCAAGCAGCAGAAGGACATGATCTC
AGATTAGAGAGAGGCCAAGAGTATCTCATTTTAGAAAAGAATGATGTTCA
TTGGTGGAGAGCAAGAGATAAATATGGGAATGAAGGATATATCCCAAGTA
ATTACGTAACGGGAAAGAAATCAAACAACTTAGATCAATATGAATGGTAT
TGCAGAAATATGAATAGAAGCAAGGCAGAGCAACTCCTCCGCAGTGAAGA
TAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTGTACA
CAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGG
CATTATCATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGC
TGAAAAACATGCTTTTGGCTCCATTCCTGAGATTATTGAATATCATAAGC
ACAATGCAGCAGGACTTGTCACCAGGCTTCGGTACCCAGTTAGTGTGAAA
GGGAAGAATGCACCCACCACTGCAGGATTCAGCTATGAGAAATGGGAGAT
TAACCCTTCAGAACTGACCTTTATGAGGGAATTGGGAAGTGGACTGTTTG
GAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAATCAAA
GCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAA
AGTGATGATGAAACTGACACACCCGAAGTTAGTGCAGCTTTATGGTGTGT
GCACCCAGCAGAAACCAATATACATTGTTACTGAGTTCATGGAAAGGGGC
TGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCATTTCAGTAGAGACGT
ACTGCTGAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTGGAGA
GAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCTAGTAAGT
GAGGCGGGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCT
GGATGATCAGTACACAAGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGT
GTCCACCTGAAGTGTTTAATTACAGCCGCTTCAGCAGCAAATCAGATGTC
TGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACGGAAGGCAGAATGCC
TTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGAGGCC
ACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATG
CTGAGATGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCT
GCTGCGCACAATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGAAGAT
AAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMNFNTILEEILIKRSQQKKKTS
PLNYKERLFVLTKSMLTYYEGRAEKKYRKGFIDVSKIKCVEIVKNDDGVI
PCQNKYPFQVVHDANTLYIFAPSPQSRDLWVKKLKEEIKNNNNIMIKYHP
KFWTDGSYQCCRQTEKLAPGCEKYNLFESSIRKALPPAPETKKRRPPPPI
PLEEEDNSEEIVVAMYDFQAAEGHDLRLERGQEYLILEKNDVHWWRARDK
YGNEGYIPSNYVTGKKSNNLDQYEWYCRNMNRSKAEQLLRSEDKEGGFMV
RDSSQPGLYTVSLYTKFGGEGSSGFRHYHIKETTTSPKKYYLAEKHAFGS
IPEIIEYHKHNAAGLVTRLRYPVSVKGKNAPTTAGFSYEKWEINPSELTF
MRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKLTH
PKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDVLLSMCQD
VCEGMEYLERNSFIHRDLAARNCLVSEAGVVKVSDFGMARYVLDDQYTSS
SGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEVFTEGRMPFEKYTNY
EVVTMVTRGHRLYQPKLASNYVYEVMLRCWQEKPEGRPSFEDLLRTIDEL
VECEETFGR*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU36461
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; ; Estimated MW of expressed protein : 76951 Daltons