cDNA Clone TEC

• Plasmid: pGEX6P-3-TEC (tec protein tyrosine kinase)
• Parent Plasmid: pGEX6P3
• Expressed: GST-TEC
• Genbank: NM_003215.2 GI:130977758
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTC
ACAGCAGAAAAAGAAGACATCGCCCTTAAACTACAAAGAGAGACTTTTTG
TACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAAGAAA
TACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGT
GAAGAATGATGATGGTGTCATTCCCTGTCAAAATAAGTATCCATTTCAGG
TTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGTCCACAAAGC
AGGGACCTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAA
TATTATGATTAAATATCATCCTAAATTCTGGACAGATGGAAGTTATCAGT
GTTGTAGACAAACTGAAAAATTAGCACCCGGATGTGAAAAATACAATCTT
TTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCACCAGAAACAAAGAA
GCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGTGAAG
AAATCGTTGTAGCCATGTATGATTTCCAAGCAGCAGAAGGACATGATCTC
AGATTAGAGAGAGGCCAAGAGTATCTCATTTTAGAAAAGAATGATGTTCA
TTGGTGGAGAGCAAGAGATAAATATGGGAATGAAGGATATATCCCAAGTA
ATTACGTAACGGGAAAGAAATCAAACAACTTAGATCAATATGAATGGTAT
TGCAGAAATATGAATAGAAGCAAGGCAGAGCAACTCCTCCGCAGTGAAGA
TAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTGTACA
CAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGG
CATTATCATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGC
TGAAAAACATGCTTTTGGCTCCATTCCTGAGATTATTGAATATCATAAGC
ACAATGCAGCAGGACTTGTCACCAGGCTTCGGTACCCAGTTAGTGTGAAA
GGGAAGAATGCACCCACCACTGCAGGATTCAGCTATGAGAAATGGGAGAT
TAACCCTTCAGAACTGACCTTTATGAGGGAATTGGGAAGTGGACTGTTTG
GAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAATCAAA
GCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAA
AGTGATGATGAAACTGACACACCCGAAGTTAGTGCAGCTTTATGGTGTGT
GCACCCAGCAGAAACCAATATACATTGTTACTGAGTTCATGGAAAGGGGC
TGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCATTTCAGTAGAGACGT
ACTGCTGAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTGGAGA
GAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCTAGTAAGT
GAGGCGGGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCT
GGATGATCAGTACACAAGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGT
GTCCACCTGAAGTGTTTAATTACAGCCGCTTCAGCAGCAAATCAGATGTC
TGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACGGAAGGCAGAATGCC
TTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGAGGCC
ACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATG
CTGAGATGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCT
GCTGCGCACAATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGAAGAT
AAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNFNTILEEILIKRSQQKK
KTSPLNYKERLFVLTKSMLTYYEGRAEKKYRKGFIDVSKIKCVEIVKNDD
GVIPCQNKYPFQVVHDANTLYIFAPSPQSRDLWVKKLKEEIKNNNNIMIK
YHPKFWTDGSYQCCRQTEKLAPGCEKYNLFESSIRKALPPAPETKKRRPP
PPIPLEEEDNSEEIVVAMYDFQAAEGHDLRLERGQEYLILEKNDVHWWRA
RDKYGNEGYIPSNYVTGKKSNNLDQYEWYCRNMNRSKAEQLLRSEDKEGG
FMVRDSSQPGLYTVSLYTKFGGEGSSGFRHYHIKETTTSPKKYYLAEKHA
FGSIPEIIEYHKHNAAGLVTRLRYPVSVKGKNAPTTAGFSYEKWEINPSE
LTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMK
LTHPKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDVLLSM
CQDVCEGMEYLERNSFIHRDLAARNCLVSEAGVVKVSDFGMARYVLDDQY
TSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEVFTEGRMPFEKY
TNYEVVTMVTRGHRLYQPKLASNYVYEVMLRCWQEKPEGRPSFEDLLRTI
DELVECEETFGR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36420
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 100403 Daltons