cDNA Clone USP27X

• Synonyms: USP22L, USP27
• Plasmid: pFastbac GST 6P1 USP27X
• Parent Plasmid: pFastbac GST
• Expressed: GST-USP27X
• Genbank: NM_001145073.1 GI:223005905
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTGTAAGGACTATGTATATGACAAAGACATTGAGCAAATTGC
CAAAGAAGAGCAAGGAGAAGCTTTGAAATTACAAGCCTCCACCTCAACAG
AGGTTTCTCACCAGCAGTGTTCAGTGCCAGGCCTTGGTGAGAAATTCCCA
ACCTGGGAAACAACCAAACCAGAATTAGAACTGCTGGGGCACAACCCGAG
GAGAAGAAGAATCACCTCCAGCTTTACGATCGGTTTAAGAGGACTCATCA
ATCTTGGCAACACGTGCTTTATGAACTGCATTGTCCAGGCCCTCACCCAC
ACGCCGATACTGAGAGATTTCTTTCTCTCTGACAGGCACCGATGTGAGAT
GCCGAGTCCCGAGTTGTGTCTGGTCTGTGAGATGTCGTCGCTGTTTCGGG
AGTTGTATTCTGGAAACCCGTCTCCTCATGTGCCCTATAAGTTACTGCAC
CTGGTGTGGATACATGCCCGCCATTTAGCAGGGTACAGGCAACAGGATGC
CCACGAGTTCCTCATTGCAGCGTTAGATGTCCTGCACAGGCACTGCAAAG
GTGATGATGTCGGGAAGGCGGCCAACAATCCCAACCACTGTAACTGCATC
ATAGACCAAATCTTCACAGGTGGCCTGCAGTCTGATGTCACCTGTCAAGC
CTGCCATGGCGTCTCCACCACGATAGACCCATGCTGGGACATTAGTTTGG
ACTTGCCTGGCTCTTGCACCTCCTTCTGGCCCATGAGCCCAGGGAGGGAG
AGCAGTGTGAACGGGGAAAGCCACATACCAGGAATCACCACCCTCACGGA
CTGCTTGCGGAGGTTTACGAGGCCAGAGCACTTAGGAAGCAGTGCCAAAA
TCAAATGTGGTAGTTGCCAAAGCTACCAGGAATCTACCAAACAGCTCACA
ATGAATAAATTACCTGTCGTTGCCTGTTTTCATTTCAAACGGTTTGAACA
TTCAGCGAAACAGAGGCGCAAGATCACTACATACATTTCCTTTCCTCTGG
AGCTGGATATGACGCCGTTTATGGCCTCAAGTAAAGAGAGCAGAATGAAT
GGACAATTGCAGCTGCCAACCAATAGTGGAAACAACGAAAATAAGTATTC
CTTGTTTGCTGTGGTTAATCACCAAGGAACCTTGGAGAGTGGCCACTATA
CCAGCTTCATCCGGCACCACAAGGACCAGTGGTTCAAGTGTGATGATGCC
GTCATCACTAAGGCCAGTATTAAGGACGTACTGGACAGTGAAGGGTATTT
ACTGTTCTATCACAAACAGGTGCTAGAACATGAGTCAGAAAAAGTGAAAG
AAATGAACACACAAGCCTACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMCKDYVYDKDIEQIAKEEQ
GEALKLQASTSTEVSHQQCSVPGLGEKFPTWETTKPELELLGHNPRRRRI
TSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRDFFLSDRHRCEMPSPE
LCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQDAHEFL
IAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGV
STTIDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRR
FTRPEHLGSSAKIKCGSCQSYQESTKQLTMNKLPVVACFHFKRFEHSAKQ
RRKITTYISFPLELDMTPFMASSKESRMNGQLQLPTNSGNNENKYSLFAV
VNHQGTLESGHYTSFIRHHKDQWFKCDDAVITKASIKDVLDSEGYLLFYH
KQVLEHESEKVKEMNTQAY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU36347
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 76453 Daltons