cDNA Clone PSKH1

• Plasmid: pGEX6P-3-PSKH1
• Parent Plasmid: pGEX6P3
• Expressed: GST-PSKH1
• Genbank: NM_006742.2 GI:194097444
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGGCTGTGGGACAAGCAAGGTCCTTCCCGAGCCACCCAAGGA
TGTCCAGCTGGATCTGGTCAAGAAGGTGGAGCCCTTCAGTGGCACTAAGA
GTGACGTGTACAAGCACTTCATCACAGAGGTGGACAGTGTTGGCCCTGTC
AAAGCCGGGTTCCCAGCAGCAAGTCAGTATGCACACCCCTGCCCCGGTCC
CCCGACTGCTGGCCACACGGAGCCTCCCTCAGAACCACCACGCAGGGCCA
GGGTAGCTAAGTACAGGGCCAAGTTTGACCCACGTGTTACAGCTAAGTAT
GACATCAAGGCCCTAATTGGCCGAGGCAGCTTCAGCCGAGTGGTACGTGT
AGAGCACCGGGCAACCCGGCAGCCGTATGCCATCAAGATGATTGAGACCA
AGTACCGGGAGGGGCGGGAGGTGTGTGAGTCGGAGCTGCGTGTGCTGCGT
CGGGTGCGTCATGCCAACATCATCCAGCTGGTGGAGGTGTTCGAGACACA
GGAGCGGGTGTACATGGTGATGGAGCTGGCCACTGGTGGAGAGCTCTTTG
ACCGCATCATTGCCAAGGGCTCCTTCACCGAGCGTGACGCCACGCGGGTG
CTGCAGATGGTGCTGGATGGCGTCCGGTATCTGCATGCACTGGGCATCAC
ACACCGAGACCTCAAACCTGAGAATCTGCTCTACTACCATCCGGGCACTG
ACTCCAAGATCATCATCACCGACTTCGGCCTGGCCAGTGCTCGCAAGAAG
GGTGATGACTGCTTGATGAAGACCACCTGTGGCACGCCTGAGTACATTGC
CCCAGAAGTCCTGGTCCGCAAGCCATACACCAACTCAGTGGACATGTGGG
CGCTGGGCGTCATTGCCTACATCCTACTCAGTGGCACCATGCCGTTTGAG
GATGACAACCGTACCCGGCTGTACCGGCAGATCCTCAGGGGCAAGTACAG
TTACTCTGGGGAGCCCTGGCCTAGTGTGTCCAACCTGGCCAAGGACTTCA
TTGACCGCCTGCTGACAGTGGACCCTGGAGCCCGTATGACTGCACTGCAG
GCCCTGAGGCACCCGTGGGTGGTGAGCATGGCTGCCTCTTCATCCATGAA
GAACCTGCACCGCTCCATATCCCAGAACCTCCTTAAACGTGCCTCCTCGC
GCTGCCAGAGCACCAAATCTGCCCAGTCCACGCGTTCCAGCCGCTCCACA
CGCTCCAATAAGTCACGCCGTGTGCGGGAACGGGAGCTGCGGGAGCTCAA
CCTGCGCTACCAGCAGCAATACAATGGCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMGCGTSKVLPEPPKDVQLD
LVKKVEPFSGTKSDVYKHFITEVDSVGPVKAGFPAASQYAHPCPGPPTAG
HTEPPSEPPRRARVAKYRAKFDPRVTAKYDIKALIGRGSFSRVVRVEHRA
TRQPYAIKMIETKYREGREVCESELRVLRRVRHANIIQLVEVFETQERVY
MVMELATGGELFDRIIAKGSFTERDATRVLQMVLDGVRYLHALGITHRDL
KPENLLYYHPGTDSKIIITDFGLASARKKGDDCLMKTTCGTPEYIAPEVL
VRKPYTNSVDMWALGVIAYILLSGTMPFEDDNRTRLYRQILRGKYSYSGE
PWPSVSNLAKDFIDRLLTVDPGARMTALQALRHPWVVSMAASSSMKNLHR
SISQNLLKRASSRCQSTKSAQSTRSSRSTRSNKSRRVRERELRELNLRYQ
QQYNG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36107
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 74857 Daltons