cDNA Clone PSKH2

• Plasmid: pGEX6P-2-PSKH2
• Parent Plasmid: pGEX6P2
• Expressed: GST-PSKH2
• Genbank: NM_033126.1 GI:14916454*
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGGGTGCGGCGCCAGCAGGAAGGTGGTCCCGGGGCCACC
AGCGCTGGCTTGGGCCAAGCACGAAGGTCAAAACCAAGCCGGCGTCGGAG
GCGCGGGGCCTGGGCCCGAGGCGGCGGCCCAGGCGGCGCAGAGGATACAG
GTGGCTCGCTTCCGAGCCAAGTTCGACCCCCGGGTCCTTGCCAGATATGA
CATCAAAGCTCTTATTGGGACAGGCAGTTTCAGCAGGGTTGTCAGGGTAG
AGCAGAAGACCACCAAGAAACCTTTTGCAATAAAAGTGATGGAAACCAGA
GAGAGGGAAGGTAGAGAAGCGTGCGTGTCTGAGCTGAGCGTCCTGCGGCG
GGTTAGCCATCGTTACATTGTCCAGCTCATGGAGATCTTTGAGACTGAGG
ATCAAGTTTACATGGTAATGGAGCTGGCTACCGGAGGGGAGCTCTTTGAT
CGACTCATTGCTCAGGGATCCTTTACAGAGCGGGATGCCGTCAGGATCCT
CCAGATGGTTGCTGATGGGATTAGGTATTTGCATGCGCTGCAGATAACTC
ATAGGAATCTAAAGCCTGAAAACCTCTTATACTATCATCCAGGTGAAGAG
TCGAAAATTTTAATTACAGATTTTGGTTTGGCATACTCCGGGAAAAAAAG
TGGTGACTGGACAATGAAGACACTCTGTGGGACCCCAGAGTACATAGCTC
CTGAGGTTTTGCTAAGGAAGCCTTATACCAGTGCAGTGGACATGTGGGCT
CTTGGTGTGATCACATATGCTTTACTTAGCGGATTCCTGCCTTTTGATGA
TGAAAGCCAGACAAGGCTTTACAGGAAGATTCTGAAAGGCAAATATAATT
ATACAGGAGAGCCTTGGCCAAGCATTTCCCACTTGGCGAAGGACTTTATA
GACAAACTACTGATTTTGGAGGCTGGTCATCGCATGTCAGCTGGCCAGGC
CCTGGACCATCCCTGGGTGATCACCATGGCTGCAGGGTCTTCCATGAAGA
ATCTCCAGAGGGCCATATCCCGAAACCTCATGCAGAGGGCCTCTCCCCAC
TCTCAGAGTCCTGGATCTGCACAGTCTTCTAAGTCACATTATTCTCACAA
ATCCAGGCATATGTGGAGCAAGAGAAACTTAAGGATAGTAGAATCGCCAC
TGTCTGCGCTTTTGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMGCGASRK
VVPGPPALAWAKHEGQNQAGVGGAGPGPEAAAQAAQRIQVARFRAKFDPR
VLARYDIKALIGTGSFSRVVRVEQKTTKKPFAIKVMETREREGREACVSE
LSVLRRVSHRYIVQLMEIFETEDQVYMVMELATGGELFDRLIAQGSFTER
DAVRILQMVADGIRYLHALQITHRNLKPENLLYYHPGEESKILITDFGLA
YSGKKSGDWTMKTLCGTPEYIAPEVLLRKPYTSAVDMWALGVITYALLSG
FLPFDDESQTRLYRKILKGKYNYTGEPWPSISHLAKDFIDKLLILEAGHR
MSAGQALDHPWVITMAAGSSMKNLQRAISRNLMQRASPHSQSPGSAQSSK
SHYSHKSRHMWSKRNLRIVESPLSALL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36100
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_033126.1 CDS:; g186a (silent); Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 70827 Daltons