cDNA Clone RSK2

• Synonyms: RPS6KA3, MAPKAP-K1b, CLS, HU-3, ISPK-1, MRX19, p90-RSK2, pp90RSK2, RSK, RSK2, S6K-alpha3
• Plasmid: pETBlue2.6His-6P RSK2
• Parent Plasmid: pETBlue2 6His
• Expressed: 6His-RSK2
• Genbank: NM_004586.2
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCCGCTGGCGCAGCTGGCGGACCCGTGGCAGAAGATGGCTGT
GGAGAGCCCGTCCGACAGCGCTGAGAATGGACAGCAAATTATGGATGAAC
CTATGGGAGAGGAGGAGATTAACCCACAAACTGAAGAAGTCAGTATCAAA
GAAATTGCAATCACACATCATGTAAAGGAAGGACATGAAAAGGCAGATCC
TTCCCAGTTTGAACTTTTAAAAGTATTAGGGCAGGGATCATTTGGAAAGG
TTTTCTTAGTTAAAAAAATCTCAGGCTCTGATGCTAGGCAGCTTTATGCC
ATGAAGGTATTGAAGAAGGCCACACTGAAAGTTCGAGACCGAGTTCGGAC
AAAAATGGAACGTGATATCTTGGTAGAGGTTAATCATCCTTTTATTGTCA
AGTTGCATTATGCTTTTCAAACTGAAGGGAAGTTGTATCTTATTTTGGAT
TTTCTCAGGGGAGGAGATTTGTTTACACGCTTATCCAAAGAGGTGATGTT
CACAGAAGAAGATGTCAAATTCTACTTGGCTGAACTTGCACTTGCTTTAG
ACCATCTACATAGCCTGGGAATAATTTATAGAGACTTAAAACCAGAAAAT
ATACTTCTTGATGAAGAAGGTCACATCAAGTTAACAGATTTCGGCCTAAG
TAAAGAGTCTATTGACCATGAAAAGAAGGCATATTCTTTTTGTGGAACTG
TGGAGTATATGGCTCCAGAAGTAGTTAATCGTCGAGGTCATACTCAGAGT
GCTGACTGGTGGTCTTTTGGTGTGTTAATGTTTGAAATGCTTACTGGTAC
ACTACCTTTCCAAGGAAAAGATCGAAAAGAAACAATGACTATGATTCTTA
AAGCCAAACTTGGAATGCCACAGTTTTTGAGTCCTGAAGCGCAGAGTCTT
TTACGAATGCTTTTCAAGCGAAATCCTGCAAACAGATTAGGTGCAGGACC
AGATGGAGTTGAAGAAATTAAAAGACATTCATTTTTCTCAACGATAGACT
GGAATAAACTGTATAGAAGAGAAATTCATCCGCCATTTAAACCTGCAACG
GGCAGGCCTGAAGATACATTCTATTTTGATCCTGAGTTTACTGCAAAAAC
TCCCAAAGATTCACCTGGCATTCCACCTAGTGCTAATGCACATCAGCTTT
TTCGGGGGTTTAGTTTTGTTGCTATTACCTCAGATGATGAAAGCCAAGCT
ATGCAGACAGTTGGTGTACATTCAATTGTTCAGCAGTTACACAGGAACAG
TATTCAGTTTACTGATGGATATGAAGTAAAAGAAGATATTGGAGTTGGCT
CCTACTCTGTTTGCAAGAGATGTATACATAAAGCTACAAACATGGAGTTT
GCAGTGAAGATTATTGATAAAAGCAAGAGAGACCCAACAGAAGAAATTGA
AATTCTTCTTCGTTATGGACAGCATCCAAACATTATCACTCTAAAGGATG
TATATGATGATGGAAAGTATGTGTATGTAGTAACAGAACTTATGAAAGGA
GGTGAATTGCTGGATAAAATTCTTAGACAAAAATTTTTCTCTGAACGAGA
GGCCAGTGCTGTCCTGTTCACTATAACTAAAACCGTTGAATATCTTCACG
CACAAGGGGTGGTTCATAGAGACTTGAAACCTAGCAACATTCTTTATGTG
GATGAATCTGGTAATCCGGAATCTATTCGAATTTGTGATTTTGGCTTTGC
AAAACAGCTGAGAGCGGAAAATGGTCTTCTCATGACTCCTTGTTACACTG
CAAATTTTGTTGCACCAGAGGTTTTAAAAAGACAAGGCTATGATGCTGCT
TGTGATATATGGAGTCTTGGTGTCCTACTCTATACAATGCTTACCGGTTA
CACTCCATTTGCAAATGGTCCTGATGATACACCAGAGGAAATATTGGCAC
GAATAGGTAGCGGAAAATTCTCACTCAGTGGTGGTTACTGGAATTCTGTT
TCAGACACAGCAAAGGACCTGGTGTCAAAGATGCTTCATGTAGACCCTCA
TCAGAGACTGACTGCTGCTCTTGTGCTCAGACATCCTTGGATCGTCCACT
GGGACCAACTGCCACAATACCAACTAAACAGACAGGATGCACCACATCTA
GTAAAGGGTGCCATGGCAGCTACATATTCTGCTTTGAACCGTAATCAGTC
ACCAGTTTTGGAACCAGTAGGCCGCTCTACTCTTGCTCAGCGGAGAGGTA
TTAAAAAAATCACCTCAACAGCCCTGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMPLAQLADPWQKMAVESPSDSAENGQQ
IMDEPMGEEEINPQTEEVSIKEIAITHHVKEGHEKADPSQFELLKVLGQG
SFGKVFLVKKISGSDARQLYAMKVLKKATLKVRDRVRTKMERDILVEVNH
PFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAEL
ALALDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKESIDHEKKAYS
FCGTVEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGTLPFQGKDRKETM
TMILKAKLGMPQFLSPEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHSFF
STIDWNKLYRREIHPPFKPATGRPEDTFYFDPEFTAKTPKDSPGIPPSAN
AHQLFRGFSFVAITSDDESQAMQTVGVHSIVQQLHRNSIQFTDGYEVKED
IGVGSYSVCKRCIHKATNMEFAVKIIDKSKRDPTEEIEILLRYGQHPNII
TLKDVYDDGKYVYVVTELMKGGELLDKILRQKFFSEREASAVLFTITKTV
EYLHAQGVVHRDLKPSNILYVDESGNPESIRICDFGFAKQLRAENGLLMT
PCYTANFVAPEVLKRQGYDAACDIWSLGVLLYTMLTGYTPFANGPDDTPE
EILARIGSGKFSLSGGYWNSVSDTAKDLVSKMLHVDPHQRLTAALVLRHP
WIVHWDQLPQYQLNRQDAPHLVKGAMAATYSALNRNQSPVLEPVGRSTLA
QRRGIKKITSTAL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36056
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct