cDNA Clone TESK1

• Plasmid: pGEX6P-3-TESK1
• Parent Plasmid: pGEX6P2
• Expressed: GST-TESK1
• Genbank: NM_006285.2 GI:66932998*
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGGCCGGGGAACGGCCCCCACTGCGGGGCCCTGGGCCCGGGCC
TGGAGAGGTGCCGGGGGAGGGGCCCCCGGGGCCGGGGGGCACGGGCGGAG
GCCCGGGCCGGGGCCGCCCCTCCTCCTACCGGGCTCTCCGCAGCGCCGTG
TCTAGCCTGGCGCGTGTGGACGATTTTCACTGCGCGGAGAAGATCGGGGC
CGGCTTCTTCTCTGAGGTCTACAAGGTTCGGCACCGACAGTCAGGGCAAG
TCATGGTGCTGAAGATGAACAAGCTCCCCAGTAACCGGGGCAACACACTA
CGGGAAGTGCAGCTGATGAACCGGCTCAGGCACCCCAACATCCTAAGGTT
CATGGGAGTCTGTGTGCACCAGGGACAGCTGCACGCTCTTACAGAGTATA
TGAATGGGGGGACATTGGAACAGCTGCTCAGCTCCCCTGAACCCCTATCC
TGGCCGGTCAGGCTCCACCTGGCCCTGGACATTGCCCGAGGCCTGCGGTA
CCTGCACTCCAAAGGTGTATTTCACCGCGACCTCACATCCAAGAACTGTC
TAGTCCGACGGGAAGATCGAGGCTTCACCGCTGTCGTGGGTGACTTCGGG
CTGGCCGAAAAGATTCCTGTGTATAGGGAGGGGGCAAGGAAGGAGCCATT
GGCCGTGGTGGGCTCCCCATACTGGATGGCTCCAGAGGTGTTACGGGGTG
AGCTGTATGATGAGAAGGCTGATGTCTTTGCCTTCGGGATTGTCCTCTGT
GAGCTCATCGCCCGAGTACCTGCAGACCCTGACTACCTACCACGCACTGA
GGACTTTGGCCTGGATGTGCCTGCTTTCCGAACTCTGGTGGGGGATGACT
GCCCACTGCCTTTCTTGCTCCTGGCCATCCACTGCTGCAACCTGGAACCC
AGCACCCGTGCCCCCTTCACCGAAATTACCCAGCACCTGGAATGGATCCT
GGAGCAGCTGCCTGAGCCAGCCCCACTCACCAGGACCGCCCTGACACACA
ATCAGGGCTCTGTTGCAAGAGGGGGTCCCTCTGCCACGCTTCCCAGGCCA
GATCCCCGGCTTTCCCGAAGCCGGTCAGACCTCTTCCTGCCCCCATCACC
AGAATCACCCCCCAACTGGGGGGACAATCTGACTCGAGTCAACCCCTTCT
CACTACGGGAAGACCTCAGGGGTGGCAAGATCAAGCTCTTAGACACACCC
AGCAAGCCAGTCCTGCCTCTTGTGCCACCATCACCATTCCCATCCACTCA
GCTGCCCTTGGTGACCACTCCGGAGACCCTGGTCCAGCCTGGGACACCTG
CCCGCCGCTGCCGCTCACTACCCTCATCCCCCGAGCTCCCCCGCCGTATG
GAGACAGCACTGCCAGGTCCTGGCCCTCCCGCTGTGGGCCCCTCGGCTGA
AGAGAAGATGGAGTGCGAGGGCAGCAGCCCTGAGCCGGAACCTCCAGGGC
CAGCGCCCCAGCTGCCTCTGGCTGTGGCCACAGACAACTTCATCAGCACC
TGTTCCTCGGCCTCCCAACCCTGGTCCCCTAGATCAGGACCCGTCCTCAA
TAACAATCCCCCAGCTGTGGTGGTGAACTCCCCACAAGGCTGGGCTGGGG
AGCCCTGGAACCGGGCCCAGCATAGCCTGCCCCGGGCGGCAGCCCTGGAG
CGGACAGAACCCTCGCCACCCCCTTCAGCTCCCCGGGAGCCCGATGAGGG
GCTGCCCTGTCCTGGCTGCTGCCTCGGCCCCTTCAGCTTTGGCTTCCTGT
CCATGTGCCCCCGCCCCACACCAGCTGTTGCCCGCTACCGCAACCTGAAC
TGTGAGGCGGGCAGTCTCCTCTGCCACCGAGGGCACCACGCCAAGCCACC
CACACCCAGCCTGCAGCTGCCTGGGGCACGCTCTTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGERPPLRGPGPGPGEVP
GEGPPGPGGTGGGPGRGRPSSYRALRSAVSSLARVDDFHCAEKIGAGFFS
EVYKVRHRQSGQVMVLKMNKLPSNRGNTLREVQLMNRLRHPNILRFMGVC
VHQGQLHALTEYMNGGTLEQLLSSPEPLSWPVRLHLALDIARGLRYLHSK
GVFHRDLTSKNCLVRREDRGFTAVVGDFGLAEKIPVYREGARKEPLAVVG
SPYWMAPEVLRGELYDEKADVFAFGIVLCELIARVPADPDYLPRTEDFGL
DVPAFRTLVGDDCPLPFLLLAIHCCNLEPSTRAPFTEITQHLEWILEQLP
EPAPLTRTALTHNQGSVARGGPSATLPRPDPRLSRSRSDLFLPPSPESPP
NWGDNLTRVNPFSLREDLRGGKIKLLDTPSKPVLPLVPPSPFPSTQLPLV
TTPETLVQPGTPARRCRSLPSSPELPRRMETALPGPGPPAVGPSAEEKME
CEGSSPEPEPPGPAPQLPLAVATDNFISTCSSASQPWSPRSGPVLNNNPP
AVVVNSPQGWAGEPWNRAQHSLPRAAALERTEPSPPPSAPREPDEGLPCP
GCCLGPFSFGFLSMCPRPTPAVARYRNLNCEAGSLLCHRGHHAKPPTPSL
QLPGARS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31976
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_006285.2 CDS:; g441a (silent); Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 94505 Daltons