cDNA Clone PPP1R3A

• Synonyms: GM; PP1G; PPP1R3
• Plasmid: pCMV5 HA PPP1R3A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA PPP1R3A
• Genbank: NM_002711.4
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGAGCCTTCTGAAGTACCTAGTCAGATTAGCAAAGATAATTTTTTAGAAGTTCCTAATTTATCTGACTCTCTTTGTGAAGATGAAGAAGTTACTTTCCAACCTGGTTTCTCCCCTCAACCAAGTAGACGAGGTTCTGATTCTTCTGAAGACATATACCTGGATACCCCATCTTCAGGTACTAGAAGAGTTTCATTTGCTGATTCCTTTGGATTCAATCTTGTGTCTGTTAAAGAATTTGATTGCTGGGAATTACCGAGTGCTTCAACCACTTTTGACTTAGGGACGGACATTTTCCACACAGAAGAATATGTTTTAGCCCCACTGTTTGACTTGCCTTCTTCAAAAGAAGATCTTATGCAACAACTCCAAATACAGAAAGCAATACTGGAGTCAACTGAGTCTCTTCTTGGGTCTACAAGTATCAAGGGTATTATTCGAGTTTTGAATGTTTCTTTTGAGAAGTTAGTATATGTAAGAATGTCTTTAGATGACTGGCAGACACATTATGACATTTTAGCAGAATATGTTCCTAATTCATGTGATGGTGAAACTGACCAGTTCTCCTTTAAGATTGTATTGGTTCCTCCTTATCAAAAAGATGGCAGTAAAGTTGAGTTTTGTATACGTTATGAAACTTCTGTTGGTACATTTTGGTCAAATAATAATGGCACAAATTATACATTCATTTGTCAAAAGAAAGAACAAGAGCCGGAGCCTGTAAAACCATGGAAAGAAGTTCCTAACAGACAAATAAAAGGCTGCTTAAAGGTAAAATCAAGTAAAGAAGAATCATCAGTAACATCAGAAGAAAATAACTTTGAGAATCCAAAGAATACAGATACCTATATCCCAACAATCATTTGTTCTCATGAGGACAAGGAAGATTTGGAAGCCAGTAATCGAAATGTAAAAGATGTAAACAGGGAACATGATGAACATAATGAAAAAGAATTAGAGTTGATGATAAATCAACACTTAATAAGAACCAGAAGTACTGCTTCCAGAGATGAAAGGAATACATTTTCAACAGATCCAGTCAATTTTCCAAATAAAGCAGAGGGGTTAGAGAAGAAGCAAATCCATGGTGAAATATGTACTGACTTGTTCCAAAGGTCTCTGTCTCCAAGTTCATCAGCAGAAAGCTCCGTAAAGGGAGATTTTTACTGCAATGAAAAATATTCCTCAGGAGATGACTGTACACATCAACCTTCAGAGGAAACTACTTCAAATATGGGAGAAATCAAGCCATCATTGGGAGATACTAGTAGTGATGAACTAGTGCAATTACATACTGGCAGCAAAGAAGTCCTGGATGATAATGCTAATCCAGCCCATGGCAATGGCACAATGCAAATACCTTGCCCCTCTTCAGATCAACTAATGGCAGGAAACCTTAATAAAAAACATGAAGGAGGAGCTAAAAAAATTGAAGTAAAAGATTTGGGATGTTTACGAAGAGATTTCCATTCAGATACGTCGGCATGTCTCAAAGAATCAACAGAAGAAGGATCTTCTAAGGAAGATTATTATGGCAATGGTAAGGATGATGAAGAACAAAGAATATATTTAGGTGTTAATGAAAAACAAAGAAAAAATTTCCAAACAATCTTACATGACCAAGAAAGGAAGATGGGTAACCCTAAAATAAGTGTGGCAGGGATTGGAGCTAGTAACAGAGACCTGGCTACTCTGCTGAGCGAACATACCGCAATCCCCACCCGGGCAATCACAGCAGATGTGTCTCATTCACCAAGGACAAATTTAAGTTGGGAAGAAGCTGTGTTAACCCCAGAGCATCATCATTTGACTAGTGAAGGCAGCGCTTTAGGAGGGATAACTGGTCAAGTTTGTTCATCAAGAACTGGAAATGTTTTGAGGAATGATTATCTTTTCCAAGTTGAAGAAAAATCAGGTGGGATTAATTCTGAAGATCAGGATAATAGCCCACAGCATAAACAAAGTTGGAATGTTCTGGAAAGTCAGGGAAAATCAAGAGAGAATAAGACAAACATAACAGAGCATATCAAAGGACAAACAGATTGTGAAGACGTGTGGGGAAAAAGAGATAATACGAGGAGTTTGAAAGCTACTACAGAAGAATTGTTTACCTGCCAAGAAACAGTGTGCTGTGAACTGTCTTCTCTAGCTGATCATGGCATTACTGAGAAAGCAGAAGCTGGTACAGCCTATATAATTAAGACAACATCAGAAAGTACTCCAGAAAGCATGTCTGCTAGAGAAAAAGCAATAATTGCTAAGCTACCTCAAGAGACAGCACGAAGTGACAGGCCCATCGAGGTAAAGGAAACAGCGTTTGATCCACATGAAGGGAGAAATGATGATTCACATTATACCCTTTGTCAACGAGATACAGTAGGTGTAATCTATGACAATGATTTTGAAAAGGAATCACGTTTAGGTATTTGTAATGTACGTGTAGATGAAATGGAGAAGGAAGAAACCATGTCTATGTACAATCCTAGGAAGACACATGACAGGGAGAAATGTGGCACTGGAAATATAACATCTGTGGAAGAATCCTCATGGGTCATTACAGAATATCAAAAAGCAACTTCAAAACTGGATTTACAGTTGGGAATGTTACCAACAGACAAAACTGTATTTTCAGAAAACAGAGATCATAGGCAGGTTCAAGAATTATCAAAGAAAACAGACTCGGATGCCATTGTGCATTCTGCTTTTAACTCAGACACTAATAGAGCTCCTCAGAATAGCTCTCCTTTTTCCAAACATCATACTGAAATTTCAGTGTCAACTAATGAGCAGGCAATTGCTGTAGAGAATGCAGTTACTACCATGGCTAGCCAACCTATTTCTACGAAATCAGAAAATATTTGTAATTCAACAAGAGAAATCCAGGGTATTGAGAAGCACCCTTATCCTGAGTCTAAACCTGAAGAAGTTTCCAGAAGTTCAGGAATAGTGACATCAGGTAGTAGAAAAGAAAGATGCATAGGCCAGATTTTCCAAACAGAAGAGTATAGTGTGGAAAAATCTCTAGGGCCAATGATTTTAATCAACAAACCTCTTGAGAATATGGAAGAAGCAAGGCATGAAAATGAAGGATTAGTAAGCTCTGGGCAATCACTATACACTTCAGGTGAAAAGGAATCTGACAGCTCTGCTTCTACTAGTCTTCCTGTTGAGGAAAGTCAAGCTCAAGGCAACGAATCTTTGTTTTCAAAATATACCAACTCTAAAATACCTTATTTCCTTTTGTTTCTGATATTTCTTATAACTGTCTACCATTATGACTTAATGATTGGCTTGACATTCTACGTTTTGTCATTGTCCTGGCTATCCTGGGAAGAGGGTAGACAAAAAGAGTCTGTCAAAAAGAAGTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMEPSEVPSQISKDNFLEVPNLSDSLCEDEEVTFQPGFSPQPSRRGSDSSEDIYLDTPSSGTRRVSFADSFGFNLVSVKEFDCWELPSA
STTFDLGTDIFHTEEYVLAPLFDLPSSKEDLMQQLQIQKAILESTESLLGSTSIKGIIRVLNVSFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQF
SFKIVLVPPYQKDGSKVEFCIRYETSVGTFWSNNNGTNYTFICQKKEQEPEPVKPWKEVPNRQIKGCLKVKSSKEESSVTSEENNFENPKNTDTYIPTII
CSHEDKEDLEASNRNVKDVNREHDEHNEKELELMINQHLIRTRSTASRDERNTFSTDPVNFPNKAEGLEKKQIHGEICTDLFQRSLSPSSSAESSVKGDF
YCNEKYSSGDDCTHQPSEETTSNMGEIKPSLGDTSSDELVQLHTGSKEVLDDNANPAHGNGTMQIPCPSSDQLMAGNLNKKHEGGAKKIEVKDLGCLRRD
FHSDTSACLKESTEEGSSKEDYYGNGKDDEEQRIYLGVNEKQRKNFQTILHDQERKMGNPKISVAGIGASNRDLATLLSEHTAIPTRAITADVSHSPRTN
LSWEEAVLTPEHHHLTSEGSALGGITGQVCSSRTGNVLRNDYLFQVEEKSGGINSEDQDNSPQHKQSWNVLESQGKSRENKTNITEHIKGQTDCEDVWGK
RDNTRSLKATTEELFTCQETVCCELSSLADHGITEKAEAGTAYIIKTTSESTPESMSAREKAIIAKLPQETARSDRPIEVKETAFDPHEGRNDDSHYTLC
QRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTGNITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDHRQVQEL
SKKTDSDAIVHSAFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSSGIVTSGSR
KERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLIT
VYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5 HA PPP1R3A64.58 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU31878
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to reference sequence there are 3 SNP at V451M, N476K and L882H.
• Low Temp: No