cDNA Clone Rhotekin

• Plasmid: pGEX6P-2-Rhotekin
• Parent Plasmid: pGEX6P2
• Expressed: GST-Rhotekin
• Genbank: NM_001015055.1 GI:62739178
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTCTCCCGAAACCACCGGAGCCGGGTCACCGTGGCCAGGGG
CTCCGCCCTGGAGATGGAGTTCAAACGCGGCCGCTTCCGACTCAGCCTCT
TCAGCGACCTGCCCGAGGACACGGAGTTGCAGAGGAAGCTAGACCATGAG
ATCCGGATGAGGGAAGGGGCCTGTAAGCTGCTGGCAGCCTGCTCCCAGCG
AGAGCAGGCTCTGGAGGCCACCAAGAGCCTGCTAGTGTGCAACAGCCGCA
TCCTCAGCTACATGGGCGAGCTGCAGCGGCGCAAGGAGGCGCAGGTGCTG
GGGAAGACAAGCCGGCGGCCTTCTGACAGTGGCCCGCCCGCTGAGCGCTC
CCCCTGCCGCGGCCGGGTCTGCATCTCTGACCTCCGGATTCCACTCATGT
GGAAGGACACAGAATATTTCAAGAACAAAGGTGACTTGCACCGCTGGGCT
GTGTTCCTGCTGCTGCAGCTGGGGGAACACATCCAGGACACAGAGATGAT
CCTAGTGGACAGGACCCTCACAGACATCTCCTTTCAGAGCAATGTGCTCT
TCGCTGAGGCGGGGCCAGACTTTGAACTGCGGTTAGAGCTGTATGGGGCC
TGTGTGGAAGAAGAGGGGGCCCTGACTGGCGGCCCCAAGAGGCTTGCCAC
CAAACTCAGCAGCTCCCTGGGCCGCTCCTCAGGGAGGCGTGTCCGGGCAT
CGCTGGACAGTGCTGGGGGTTCAGGGAGCAGTCCCATCTTGCTCCCCACC
CCAGTTGTTGGTGGTCCTCGTTACCACCTCTTGGCTCACACCACACTCAC
CCTGGCAGCAGTGCAAGATGGATTCCGCACACATGACCTCACCCTTGCCA
GTCATGAGGAGAACCCTGCCTGGCTGCCCCTTTATGGTAGCGTGTGTTGC
CGTCTGGCAGCTCAGCCTCTCTGCATGACTCAGCCCACTGCAAGTGGTAC
CCTCAGGGTGCAGCAAGCTGGGGAGATGCAGAACTGGGCACAAGTGCATG
GAGTTCTGAAAGGCACAAACCTCTTCTGTTACCGGCAACCTGAGGATGCA
GACACTGGGGAAGAGCCGCTGCTTACTATTGCTGTCAACAAGGAGACTCG
AGTCCGGGCAGGGGAGCTGGACCAGGCTCTAGGACGGCCCTTCACCCTAA
GCATCAGTAACCAGTATGGGGATGATGAGGTGACACACACCCTTCAGACA
GAAAGTCGGGAAGCACTGCAGAGCTGGATGGAGGCTCTGTGGCAGCTTTT
CTTTGACATGAGCCAATGGAAGCAGTGCTGTGATGAAATCATGAAAATTG
AAACTCCTGCTCCCCGGAAACCACCCCAAGCACTGGCAAAGCAGGGGTCC
TTGTACCATGAGATGGCTATTGAGCCGCTGGATGACATCGCAGCGGTGAC
AGACATCCTGACCCAGCGGGAGGGCGCAAGGCTGGAGACACCCCCACCCT
GGCTGGCAATGTTTACAGACCAGCCTGCCCTGCCTAACCCCTGCTCGCCT
GCCTCAGTGGCCCCAGCCCCAGACTGGACCCACCCCCTGCCCTGGGGGAG
ACCCCGAACCTTTTCCCTGGATGCTGTCCCCCCAGACCACTCCCCTAGGG
CTCGCTCGGTTGCCCCCCTCCCACCTCAGCGATCCCCACGGACCAGAGGC
CTCTGCAGCAAAGGCCAACCTCGCACTTGGCTCCAGTCACCAGTGTGAGG
ATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFSRNHRSRVTVARGSALE
MEFKRGRFRLSLFSDLPEDTELQRKLDHEIRMREGACKLLAACSQREQAL
EATKSLLVCNSRILSYMGELQRRKEAQVLGKTSRRPSDSGPPAERSPCRG
RVCISDLRIPLMWKDTEYFKNKGDLHRWAVFLLLQLGEHIQDTEMILVDR
TLTDISFQSNVLFAEAGPDFELRLELYGACVEEEGALTGGPKRLATKLSS
SLGRSSGRRVRASLDSAGGSGSSPILLPTPVVGGPRYHLLAHTTLTLAAV
QDGFRTHDLTLASHEENPAWLPLYGSVCCRLAAQPLCMTQPTASGTLRVQ
QAGEMQNWAQVHGVLKGTNLFCYRQPEDADTGEEPLLTIAVNKETRVRAG
ELDQALGRPFTLSISNQYGDDEVTHTLQTESREALQSWMEALWQLFFDMS
QWKQCCDEIMKIETPAPRKPPQALAKQGSLYHEMAIEPLDDIAAVTDILT
QREGARLETPPPWLAMFTDQPALPNPCSPASVAPAPDWTHPLPWGRPRTF
SLDAVPPDHSPRARSVAPLPPQRSPRTRGLCSKGQPRTWLQSPV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31623
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 89488 Daltons