cDNA Clone RICTOR (mouse)

• Plasmid: pcDNA5-FRT/TO-GFP RICTOR (mouse)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-RICTOR (mouse)
• Genbank: NM_030168.3 GI:168480119*
• Tag: GFP
• Species: Mouse

Sequence of Insert

GGATCCATGGCGGCGATCGGCCGCGGACGCTCTCTGAAGAACCTCCGAGT
ACGAGGGCGGAATGACAGCGGCGAGGAGAACGTCCCGCTCGATCTGACCC
GAGAACCTTCTGATAACTTGAGGGAGATTCTCCAAAATGTGGCCAAATTG
CAAGGAGTATCAAATATGAGAAAGCTGGGCCATCTGAATAACTTCACTAA
GCTTCTTTGCGATATTGGCCATAGTGAAGAAAAACTGGGCTTTAATTATG
AAGACATCATAATTTGTTTGCGCTTAGCTTTATTAAATGAGGCAAAAGAA
GTAAGAGCAGCCGGGTTGCGAGCGCTGCGCTATCTCATCCAAGATTCCAG
TATTCTCCAGAAGGTGCTAAAACTGAAAGTGGACTATTTAATAGCTAGGT
GCATTGACATTCAGCAGAGCAACGAGGTGGAGAGGACACAAGCCCTTCGC
TTAGTCAGAAAGATGATTACTGTGAATACTTCCTTGTTTCCTAGTTCTGT
GGCCAACTCATTAATTGCGGTTGGAAATGATGGGCTTCAGGAACGAGACA
GAATGGTCCAGGCTTGCATCGCCATCATCTGTGAACTAGCACTTCAGAAC
CCAGAGGTGGTAGCCCTTCGAGGAGGACTAAACACCATACTGAAAAATGT
GATTGATTGCCAATTAAGTCGAATAAATGAGGCTCTCATTACTACAATTT
TGCACCTTCTTAATCATCCAAAGACCCGGCAGTATGTGCGAGCTGATGTA
GAATTAGAGCGAATTTTAGCACCCTATACTGATTTCCACTACAGACACAG
TCCAGATACGGCTGAAGGACAGCTCAAAGAAGACAGAGAAGCACGATTTC
TAGCCAGTAAGATGGGAATCATTGCGACATTCCGCTCGTGGGCAGGTATT
ATTAATTTATGTAAGCCTGGAAATTCTGGGATACAGTCTCTAATTGGAGT
ACTTTGCATACCGAATATGGAAATAAGGCGAGGTCTGCTCGAAGTGCTCT
ATGACATCTTTCGTCTCCCTCTGCCCGTTGTGACGGATGAGTTCATAGAA
GCCCTGCTCAGTGTAGATCCAGGAAGATTCCAGGACAGTTGGAGGCTTTC
CGATGGCTTTGTGGCAGCTGAGGCAAAAACTATTCTTCCCCATCGTGCTA
GATCCAGGCCAGACCTCATGGACAATTATTTGGCTCTGATACTCTCTGCA
TTTATTCGTAATGGACTTATAGAGGGCTTGGTCGAGGTGATAACGAACAG
TGACGACCACATCTCAGTGAGGGCGACCATCCTTTTAGGAGAGCTCCTGC
ATATGGCAAACACCATCCTGCCTCACTCGCATAGCCACCATCTACACTGC
TTGCCAACCCTCATGAACATGGCTGCGTCCTTCGATATTCCCAAGGAAAA
GAGGCTGCGAGCCAGTGCAGCCCTGAACTGTTTGAACCGCTTCCATGAGA
TGAAGAAGCGAGGACCTAAGCCCTACAGCCTTCATTTAGATCACATCATT
CAGAAAGCAATCGCAACTCACCACAAGCGGGATCAGTATCTTCGAGTTCA
GAAAGATATATTTGTTCTTAAGGATACAGAGGAAGCTCTTTTAATAAACC
TTAGAGACAGCCAAGTCCTTCAGCATAAAGAGAATCTTGACTGGGATTGG
AATCTGATTGGGACCATCCTTAAGTGGCCAAATGTAAATCTAAGAAACTA
TAAAGATGAGCAGTTGCACAGGTTTGTGCGCAGACTTCTTTACTTTTACA
AGCCCAGCAGCAAACTGTACGCTAGTCTGGATCTGGACTTGGCCAAGTCC
AAGCAGCTCACAGTTGTTGGTTGTCAGTTTACAGAATTTCTGCTCGAGTC
TGAAGAGGATGGGCAAGGATACTTAGAAGATCTCGTGAAAGATATTGTTC
AGTGGCTCAATGCTTCATCTGGAGTGAAACCTGAGAGAAGCCTTCAGAAT
AATGGCTTACTGACCACCCTTAGTCAACACTACTTCTTATTTATCGGAAC
ACTCTCTTGTCATCCTCATGGAGTCAAAATGCTGGAAAAGTGCAGTGTCT
TTCAGTGCCTCCTTAATCTTTGTTCCTTGAAAAACCAAGATCATCTGATA
AAACTCACTGTTTCCAGCCTAGACTATAGCAGAGATGGATTAGCCAGAGT
CATTCTTTCAAAAATCCTCACGGCAGCCACAGATGCTTGCAGACTGTATG
CAACAAAACATTTAAGGGTTTTATTGAGAGCTAACGTTGAATTCTTCAAC
AATTGGGGAATTGAGTTACTAGTAACTCAGCTACATGATAAAAACAAAAC
AATTTCTTCTGAAGCGCTGGATATTCTTGATGAAGCTTGTGAAGACAAGG
CCAATCTTCATGCTCTTATTCAGATGAAGCCAGCCTTATCCCACCTTGGA
GACAAGGGCTTGCTTCTACTGCTGAGATTTCTTTCCATTCCGAAAGGATT
TTCTTACCTGAATGAAAGGGGTTATGTTGCAAAACAGTTGGAAAAGTGGC
ACAAGGAATATAATTCAAAGTATGTTGACTTGATTGAGGAACAACTTAAT
GAAGCCCTCACTACATACAGGAAGCCTATTGATGGTGATAACTACGTTCG
TCGCAGTAATCAAAGACTACAGCGACCTCATGTCTACCTGCCCGTACACC
TTTATGGACAACTGGTACACCACAAAACAGGCTGCCATTTACTGGAAGTA
CAGAGTATCATCACAGAGCTCTGTCACAATGTTCGCACTCCAGACTTGGA
CAAATGGGAAGACATTAAGAAGCTGAAAGCCTCTCTTTGGGCCTTGGGAA
ATATTGGCTCATCGAATTGGGGGCTTAATTTGCTACAGGAAGAAAATGTG
ATCCCAGATATACTAAAACTTGCAAAACAGTGTGAGGTCCTTTCCATCCG
AGGGACCTGTGTATATGTTCTTGGGCTCATAGCTAAAACCAAGCAAGGCT
GTGATATTTTGAAATGTCACAGCTGGGATTCTGTAAGGCACAGTCGCAAG
CATCTATGGCCAGTGGTTCCAGATGATGTGGAGCAGCTCTGCAATGAGCT
CTCATCTGTCCCAAGCACCCTCAGCTTGAACTCTGAGTCGACCAGCTCTA
GACATAACAGTGAGAGTGAGTCTGCACCATCAAGCATGTTCATGCTGGAG
GACGACCGCTTCGGCAGCACCTCCACCAGCACATTTTTCCTTGACATCAA
TGAAGATGCTGAGCCAGCATTTTATGACCGACCTGGACCCATAAAAGATA
AAAATTCATTTCCCTTTTTTGGCTCCAGCAAACTTGTAAAGAATCGTATC
TTAAATTCACTTACTTTGCCTACTAAAAAACACCGCAGTAGCAGTGATCC
AAAAGGAGGAAAACTGTCATCTGAAAATAAGACAAGCAACCGGCGAATCA
GAACACTTACGGAGCCCAGTGTTGACTTGAATCACAGCGAAGACTTCACA
AGCTCCTCAGCACAGAAATCATTACAGCTAGAGCCTTCCTTCGTTGGGAA
TAAGCACCTTGAAGACGCGGGCAGCACTCCAAGTATTGGAGAGAATGACC
TCAAATTCCCCAAGAGTTTTGGTACCGAGACTCACAGAGAAAACACAAGC
CGAGAAAGGTTGGTTGTGGAAGGCTCAGCAAGCTCCCACATCAAGATCCG
CAGCCAGAGCTTTAACACGGACACTACGACGAGTGGCATCAGCTCCATGA
GCTCGAGCCCCTCCCGAGAGACAGTCGCTGTCGACCCCACAGCTATGGAC
ACGGACTGTGGAAGTCTGAGTACCGTGGTAAGTACTAAAACTGTTAAGAC
GAGCCACTATTTGACACCACAGTCTAACCATCTGTCTCTCTCCAAATCGA
ACTCAGTGTCCCTGGTGCCACCAGGTTCTTCTCACACCCTTCCTAGAAGA
GCCCAGTCCCTTAAAGCGCCCTCCATTGCAACAATAAAAAGTCTAGCAGA
TTGCAACTTCAGCTACACAAGCTCTAGAGATGCCTTTGGCTATGCTACAT
TGAAAAGGTTACAGCAGCAAAGAATGCACCCGTCCTTGTCTCACTCGGAA
GCTTTGGCGTCGCCGGCAAAAGATGTGCTTTTTACCGACACCATCACCAT
GAAGGCCAACAGCTTTGAATCCAGACTGACGCCAAGCAGGTTTATGAAAG
CTTTAAGTTATGCGTCATTAGATAAAGAAGATTTACTAAGTCCTATTAAT
CACAATACCCTCCAGCGCTCCTCCTCAGTTAGGTCTATGGTGTCCAGTGC
TACATATGGAGGTTCCGATGACTACATTGGACTTGCTCTTCCAGTAGACA
TCAATGACATCTTCCAGATAAAGGATGTTCCTTATTTTCAATCAAAGCAC
GTGCCTCCACCAGATGACCGAGGTGCAAGAATGTTTTCCCATGATGGAGC
AGGTCTTTCATCTGGAGCCGGTGGTCTCGTAAAGAACTCTTTTCACTTGC
TGCGGCAGCAGATGAGTCTCACAGAAATAATGAACTCTGTCCATTCGGAC
GCCTCCCTGTTCTTAGAGAGCACAGAAGACACTGGGCTGCAGGAGCACAC
GGATGACAATTGCCTTTACTGCGTCTGCATTGAACTCCTGGGCTTCCAGC
CCAGTAACCAGCTAAGTTCCATCTGCAGTCACTCCGATCTTCAAGATATT
CCGTACTCCGATTGGTGCGAGCAGACGATCCACAACCCCTTAGAAGTGGT
TCCCTCTAAGTTCTCGGGGATTTCTGGCTGCAGCGATGGCGCATCTCAGG
AAGAAGGCTCAGCCAGCAGCACCAAAAGCACTGAGCTGTTACTGGGTGTT
AAAACCATTCCAGATGACACACCCATGTGCCGAATCCTGCTTCGTAAGGA
AGTGCTAAGATTAGTTGTCAATTTGAGTAGTTCAGTTTCCACCAAGTGTC
ACGAGACCGGGCTTCTGACCATTAAAGAGAAGTATCCTCAAACATTTGAT
GACATATGCCTTTATTCTGAAGTTTCTCATCTGTTGTCACACTGCACATT
TAGACTTCAGTGTCGGCGGTTCATACAAGAGTTATTTCAGGATGTACAGT
TTTTACAAATGCATGAAGAAGCAGAGGCTGTGTTGGCAATTCCACCAATT
CAGCCTATAGTTGATGAGTCTGCTGAATCCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAAIGR
GRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVAKLQGVSNMRK
LGHLNNFTKLLCDIGHSEEKLGFNYEDIIICLRLALLNEAKEVRAAGLRA
LRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITV
NTSLFPSSVANSLIAVGNDGLQERDRMVQACIAIICELALQNPEVVALRG
GLNTILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAP
YTDFHYRHSPDTAEGQLKEDREARFLASKMGIIATFRSWAGIINLCKPGN
SGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTDEFIEALLSVDPG
RFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLIE
GLVEVITNSDDHISVRATILLGELLHMANTILPHSHSHHLHCLPTLMNMA
ASFDIPKEKRLRASAALNCLNRFHEMKKRGPKPYSLHLDHIIQKAIATHH
KRDQYLRVQKDIFVLKDTEEALLINLRDSQVLQHKENLDWDWNLIGTILK
WPNVNLRNYKDEQLHRFVRRLLYFYKPSSKLYASLDLDLAKSKQLTVVGC
QFTEFLLESEEDGQGYLEDLVKDIVQWLNASSGVKPERSLQNNGLLTTLS
QHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLIKLTVSSLD
YSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLV
TQLHDKNKTISSEALDILDEACEDKANLHALIQMKPALSHLGDKGLLLLL
RFLSIPKGFSYLNERGYVAKQLEKWHKEYNSKYVDLIEEQLNEALTTYRK
PIDGDNYVRRSNQRLQRPHVYLPVHLYGQLVHHKTGCHLLEVQSIITELC
HNVRTPDLDKWEDIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLA
KQCEVLSIRGTCVYVLGLIAKTKQGCDILKCHSWDSVRHSRKHLWPVVPD
DVEQLCNELSSVPSTLSLNSESTSSRHNSESESAPSSMFMLEDDRFGSTS
TSTFFLDINEDAEPAFYDRPGPIKDKNSFPFFGSSKLVKNRILNSLTLPT
KKHRSSSDPKGGKLSSENKTSNRRIRTLTEPSVDLNHSEDFTSSSAQKSL
QLEPSFVGNKHLEDAGSTPSIGENDLKFPKSFGTETHRENTSRERLVVEG
SASSHIKIRSQSFNTDTTTSGISSMSSSPSRETVAVDPTAMDTDCGSLST
VVSTKTVKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPS
IATIKSLADCNFSYTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKD
VLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINHNTLQRSS
SVRSMVSSATYGGSDDYIGLALPVDINDIFQIKDVPYFQSKHVPPPDDRG
ARMFSHDGAGLSSGAGGLVKNSFHLLRQQMSLTEIMNSVHSDASLFLEST
EDTGLQEHTDDNCLYCVCIELLGFQPSNQLSSICSHSDLQDIPYSDWCEQ
TIHNPLEVVPSKFSGISGCSDGASQEEGSASSTKSTELLLGVKTIPDDTP
MCRILLRKEVLRLVVNLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEV
SHLLSHCTFRLQCRRFIQELFQDVQFLQMHEEAEAVLAIPPIQPIVDESA
ES*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31415
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_030168.3 CDS:; c21a (silent to kill internal Not1), ; a43g (I15V*), g472a (A158T*), g554a (R185Q*), t1213a (L405I*), t2052c (silent), g4827a (silent to kill internal BamH1); ; *These changes derive from template clone DU10469 and are present in other published sequences for Rictor.