cDNA Clone RalGAPbeta

• Plasmid: pcDNA5-FRT/TO-HA RalGAPbeta
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA RalGAPbeta
• Genbank: NM_020336.2 GI:82734234*
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTACTCTGAGTGGAGGTCACTGCATTTGGTGATTCAGAATGA
TCAAGGCCATACCAGTGTGCTGCACAGCTATCCAGAGAGCGTTGGACGAG
AGGTGGCAAATGCTGTAGTCCGTCCTCTTGGGCAGGTGTTAGGTACCCCT
TCAGTGGCTGGTAGTGAGAATTTGTTAAAAACTGACAAAGAAGTAAAATG
GACCATGGAAGTAATTTGCTATGGACTGACCCTTCCATTGGATGGAGAGA
CTGTAAAATATTGCGTTGATGTATATACAGACTGGATTATGGCTTTAGTG
TTGCCAAAAGATTCTATTCCATTGCCAGTTATTAAAGAGCCTAATCAATA
TGTTCAAACTATACTAAAACACCTACAGAATCTTTTTGTACCAAGACAGG
AACAGGGTTCCAGTCAGATTCGACTATGCTTACAGGTCCTGAGAGCCATT
CAGAAACTGGCCCGTGAGTCATCTCTCATGGCCCGAGAAACTTGGGAAGT
CTTACTGTTGTTTCTTCTGCAGATTAACGACATACTTCTGGCCCCACCAA
CTGTTCAAGGTGGCATTGCTGAGAATCTAGCAGAGAAGTTGATTGGTGTT
CTCTTTGAGGTGTGGTTACTAGCTTGTACTCGGTGCTTCCCAACACCTCC
TTATTGGAAAACAGCCAAGGAGATGGTGGCTAACTGGAGGCATCACCCAG
CAGTGGTGGAGCAGTGGAGCAAGGTCATTTGTGCACTCACTTCCAGATTG
CTACGCTTTACATATGGTCCTTCATTTCCTGCATTTAAAGTTCCCGATGA
AGATGCCAGTCTGATCCCTCCAGAAATGGATAATGAGTGTGTTGCACAGA
CGTGGTTTCGCTTTTTACACATGTTAAGTAATCCTGTGGATTTGAGTAAC
CCAGCTATTATAAGCTCTACTCCCAAATTTCAGGAACAGTTCTTGAATGT
GAGCGGAATGCCGCAAGAATTGAATCAGTATCCCTGCCTTAAACATCTGC
CTCAAATATTTTTTCGTGCCATGCGTGGAATCAGCTGTCTGGTGGATGCA
TTCTTAGGTATTTCTAGACCCCGATCAGACAGTGCTCCCCCAACACCCGT
GAATAGATTAAGTATGCCTCAAAGTGCTGCTGTCAGTACCACCCCCCCAC
ATAACCGGAGGCACCGGGCTGTTACTGTGAATAAGGCCACCATGAAGACA
AGCACAGTTAGTACTGCTCATGCCTCTAAAGTTCAGCACCAGACGTCCTC
CACCTCTCCTCTGTCAAGTCCAAATCAGACTAGTTCAGAACCCCGGCCAC
TGCCTGCCCCTCGGAGACCAAAGGTTAACAGCATCTTGAATCTCTTTGGA
TCATGGTTATTTGATGCAGCATTTGTTCACTGTAAACTTCATAATGGGAT
AAACAGAGACAGCAGCATGACTGCCATTACAACACAAGCTAGCATGGAGT
TTCGACGGAAAGGGTCACAAATGTCCACAGACACCATGGTTTCCAATCCT
ATGTTTGATGCAAGTGAATTTCCTGATAACTATGAAGCAGGAAGAGCTGA
GGCTTGTGGGACACTGTGTAGGATTTTTTGTAGCAAGAAGACTGGAGAAG
AGATTCTGCCAGCTTATTTATCCAGATTTTACATGCTTTTAATTCAAGGT
TTGCAGATAAATGATTATGTGTGCCATCCTGTCTTGGCCAGCGTTATTCT
AAACTCTCCTCCTTTGTTCTGCTGTGACTTGAAAGGGATTGATGTTGTGG
TTCCTTACTTTATTTCAGCTCTTGAAACCATTTTGCCTGACAGAGAACTC
TCAAAATTCAAAAGCTATGTAAATCCAACAGAATTGCGAAGATCCTCCAT
TAATATCCTGCTTTCTTTGTTGCCCCTCCCTCATCATTTTGGCACAGTCA
AATCTGAGGTGGTCCTGGAAGGAAAGTTTAGTAACGATGACAGCTCTTCT
TATGATAAACCAATAACTTTTCTGTCCCTGAAGTTGAGACTTGTGAATAT
ATTAATAGGTGCCTTGCAAACTGAAACGGACCCCAACAACACCCAAATGA
TATTAGGGGCAATGTTAAATATTGTTCAAGATTCAGCACTTTTGGAAGCC
ATTGGTTGCCAGATGGAGATGGGTGGTGGAGAAAATAACCTGAAGAGTCA
TAGTCGCACCAATAGTGGTATTAGTTCAGCAAGTGGTGGAAGCACGGAGC
CCACGACTCCCGATAGTGAGAGACCTGCTCAAGCTCTCTTAAGAGATTAT
GCTCTTAATACAGATTCAGCTGCTGGGCTCCTGATTCGCAGCATTCATCT
CGTCACCCAAAGACTCAACTCCCAGTGGCGCCAAGACATGAGCATATCAC
TGGCAGCTCTGGAGCTCCTCTCTGGCCTTGCAAAGGTAAAAGTGATGGTT
GACTCAGGAGACCGGAAGCGAGCCATCAGTTCTGTGTGCACCTACATTGT
TTATCAGTGTAGTCGGCCAGCTCCTTTACACTCCAGGGATCTGCACTCCA
TGATAGTGGCAGCTTTTCAGTGTCTCTGTGTCTGGCTGACAGAGCACCCT
GATATGCTTGATGAAAAGGACTGCCTTAAGGAAGTACTGGAGATTGTGGA
ACTGGGTATCTCAGGAAGTAAGTCCAAGAACAATGAGCAAGAGGTCAAGT
ACAAAGGAGATAAGGAGCCAAACCCTGCATCTATGAGGGTAAAGGATGCT
GCTGAAGCCACCCTAACATGCATTATGCAGTTGCTCGGCGCATTTCCTTC
ACCTAGTGGTCCTGCCTCTCCTTGTAGTCTTGTGAATGAGACCACTTTGA
TTAAATACTCCAGGCTGCCAACCATAAACAAGCATAGTTTCCGGTACTTT
GTCTTGGATAACAGTGTCATCCTGGCAATGCTGGAACAACCTCTTGGAAA
TGAGCAGAATGATTTTTTCCCCTCTGTCACTGTGCTGGTCCGGGGAATGT
CTGGAAGACTTGCTTGGGCACAACAGCTTTGTCTTTTACCCAGAGGAGCA
AAAGCAAATCAGAAGCTTTTTGTACCTGAACCTCGCCCAGTTCCTAAAAA
TGACGTTGGATTTAAATATTCTGTGAAACATCGGCCATTTCCTGAAGAGG
TGGACAAGATTCCTTTTGTGAAAGCAGATCTCAGCATTCCAGATTTGCAT
GAAATAGTCACTGAAGAATTAGAAGAGAGACACGAAAAATTAAGGAGTGG
CATGGCCCAGCAGATTGCTTATGAAATACACCTTGAGCAACAGAGTGAGG
AGGAATTGCAGAAGAGAAGTTTTCCTGACCCAGTTACGGATTGCAAGCCC
CCGCCTCCTGCCCAGGAATTCCAAACAGCCCGCCTTTTTCTCTCACACTT
TGGATTTTTGTCCTTAGAAGCACTGAAGGAACCTGCAAATAGTCGTCTAC
CTCCTCACCTTATTGCACTTGATTCCACGATACCTGGATTTTTTGATGAC
ATTGGGTATCTGGATCTCTTGCCATGTCGTCCTTTTGACACAGTTTTTAT
TTTCTATATGAAGCCAGGTCAGAAAACGAACCAAGAGATTTTAAAGAATG
TGGAGTCTTCCAGAACTGTTCAGCCACATTTCCTAGAATTTTTGCTTTCC
CTTGGCTGGTCAGTAGATGTGGGCAGACACCCTGGTTGGACTGGGCATGT
TTCTACCAGTTGGTCTATTAATTGTTGTGATGATGGTGAAGGATCTCAAC
AAGAAGTGATTTCCTCTGAAGATATTGGAGCTAGCATTTTCAATGGACAG
AAGAAGGTGCTGTATTATGCTGATGCCCTTACAGAAATTGCTTTTGTGGT
TCCTTCTCCTGTGGAGTCCTTAACTGATTCATTGGAAAGTAACATCTCGG
ACCAAGATAGTGATTCAAATATGGATCTTATGCCAGGAATTCTGAAACAG
CCATCCCTGACACTTGAGCTTTTCCCCAATCATACAGACAATCTTAATTC
CTCACAGAGGCTCAGTCCCAGTTCCAGAATGAGGAAGCTGCCTCAGGGTC
GCCCTGTTCCTCCCCTTGGACCTGAGACAAGAGTTTCTGTAGTCTGGGTG
GAACGCTATGATGATATAGAAAACTTTCCCCTCTCAGAGCTGATGACAGA
GATCAGTACTGGTGTGGAAACTACTGCAAATAGTAGCACTTCACTGAGAT
CTACAACTCTTGAAAAAGAAGTTCCTGTCATCTTCATCCACCCTTTAAAC
ACTGGATTATTCCGGATAAAAATTCAAGGAGCCACTGGAAAATTTAATAT
GGTCATCCCTCTTGTGGATGGGATGATTGTCAGCAGGCGAGCTCTTGGCT
TTCTGGTGAGGCAGACTGTAATTAACATTTGTAGAAGAAAGAGACTGGAA
AGTGACTCCTACAGTCCCCCCCATGTCCGCCGGAAACAGAAAATCACCGA
CATTGTCAACAAGTACCGGAACAAGCAGCTGGAGCCAGAGTTTTATACTT
CACTTTTCCAGGAGGTTGGACTCAAGAACTGCAGTTCTTAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMYSEWRSLHLVIQNDQGHTSVLHSYPESVGREVANAVV
RPLGQVLGTPSVAGSENLLKTDKEVKWTMEVICYGLTLPLDGETVKYCVD
VYTDWIMALVLPKDSIPLPVIKEPNQYVQTILKHLQNLFVPRQEQGSSQI
RLCLQVLRAIQKLARESSLMARETWEVLLLFLLQINDILLAPPTVQGGIA
ENLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWS
KVICALTSRLLRFTYGPSFPAFKVPDEDASLIPPEMDNECVAQTWFRFLH
MLSNPVDLSNPAIISSTPKFQEQFLNVSGMPQELNQYPCLKHLPQIFFRA
MRGISCLVDAFLGISRPRSDSAPPTPVNRLSMPQSAAVSTTPPHNRRHRA
VTVNKATMKTSTVSTAHASKVQHQTSSTSPLSSPNQTSSEPRPLPAPRRP
KVNSILNLFGSWLFDAAFVHCKLHNGINRDSSMTAITTQASMEFRRKGSQ
MSTDTMVSNPMFDASEFPDNYEAGRAEACGTLCRIFCSKKTGEEILPAYL
SRFYMLLIQGLQINDYVCHPVLASVILNSPPLFCCDLKGIDVVVPYFISA
LETILPDRELSKFKSYVNPTELRRSSINILLSLLPLPHHFGTVKSEVVLE
GKFSNDDSSSYDKPITFLSLKLRLVNILIGALQTETDPNNTQMILGAMLN
IVQDSALLEAIGCQMEMGGGENNLKSHSRTNSGISSASGGSTEPTTPDSE
RPAQALLRDYALNTDSAAGLLIRSIHLVTQRLNSQWRQDMSISLAALELL
SGLAKVKVMVDSGDRKRAISSVCTYIVYQCSRPAPLHSRDLHSMIVAAFQ
CLCVWLTEHPDMLDEKDCLKEVLEIVELGISGSKSKNNEQEVKYKGDKEP
NPASMRVKDAAEATLTCIMQLLGAFPSPSGPASPCSLVNETTLIKYSRLP
TINKHSFRYFVLDNSVILAMLEQPLGNEQNDFFPSVTVLVRGMSGRLAWA
QQLCLLPRGAKANQKLFVPEPRPVPKNDVGFKYSVKHRPFPEEVDKIPFV
KADLSIPDLHEIVTEELEERHEKLRSGMAQQIAYEIHLEQQSEEELQKRS
FPDPVTDCKPPPPAQEFQTARLFLSHFGFLSLEALKEPANSRLPPHLIAL
DSTIPGFFDDIGYLDLLPCRPFDTVFIFYMKPGQKTNQEILKNVESSRTV
QPHFLEFLLSLGWSVDVGRHPGWTGHVSTSWSINCCDDGEGSQQEVISSE
DIGASIFNGQKKVLYYADALTEIAFVVPSPVESLTDSLESNISDQDSDSN
MDLMPGILKQPSLTLELFPNHTDNLNSSQRLSPSSRMRKLPQGRPVPPLG
PETRVSVVWVERYDDIENFPLSELMTEISTGVETTANSSTSLRSTTLEKE
VPVIFIHPLNTGLFRIKIQGATGKFNMVIPLVDGMIVSRRALGFLVRQTV
INICRRKRLESDSYSPPHVRRKQKITDIVNKYRNKQLEPEFYTSLFQEVG
LKNCSS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31384
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Nucleotide sequence differences compared with NM_020336.2 CDS: a846g (silent), a2355g (silent)