cDNA Clone 14-3-3 beta

• Synonyms: YWHAB
• Plasmid: pEBG6P-1 14-3-3 beta
• Parent Plasmid: pEBG6P
• Expressed: GST-14-3-3 beta
• Genbank: NM_139323.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGACAATGGATAAAAGTGAGCTGGTACAGAAAGCCAAACTCGC
TGAGCAGGCTGAGCGATATGATGATATGGCTGCAGCCATGAAGGCAGTCA
CAGAACAGGGGCATGAACTCTCCAACGAAGAGAGAAATCTGCTCTCTGTT
GCCTACAAGAATGTGGTAGGCGCCCGCCGCTCTTCCTGGCGTGTCATCTC
CAGCATTGAGCAGAAAACAGAGAGGAATGAGAAGAAGCAGCAGATGGGCA
AAGAGTACCGTGAGAAGATAGAGGCAGAACTGCAGGACATCTGCAATGAT
GTTCTGGAGCTGTTGGACAAATATCTTATTCCCAATGCTACACAACCAGA
AAGTAAGGTGTTCTACTTGAAAATGAAAGGAGATTATTTTAGGTATCTTT
CTGAAGTGGCATCTGGAGACAACAAACAAACCACTGTGTCGAACTCCCAG
CAGGCTTACCAGGAAGCATTTGAAATTAGTAAGAAAGAAATGCAGCCTAC
ACACCCAATTCGTCTTGGTCTGGCACTAAATTTCTCAGTCTTTTACTATG
AGATTCTAAACTCTCCTGAAAAGGCCTGTAGCCTGGCAAAAACGGCATTT
GATGAAGCAATTGCTGAATTGGATACGCTGAATGAAGAGTCTTATAAAGA
CAGCACTCTGATCATGCAGTTACTTAGGGACAATCTCACTCTGTGGACAT
CGGAAAACCAGGGAGACGAAGGAGACGCTGGGGAGGGAGAGAACTAAGCG
GCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMTMDKSELVQKAK
LAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRV
ISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQ
PESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQ
PTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESY
KDSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31357
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express 14-3-3 beta with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 55498 Daltons