cDNA Clone PFK2

• Plasmid: pCMV5-HA PFK2 S483A
• Parent Plasmid: pCMV5
• Expressed: HA PFK2 S483A
• Genbank: NM_006212.2 GI:64762405*
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCGCCACCATGTACCCATACGATGTGCCAGATTACGCCTCTGGGGC
ATCTTCCTCAGAACAGAACAACAACAGCTATGAAACCAAAACCCCAAATC
TTCGAATGTCAGAGAAGAAATGTTCATGGGCCTCCTACATGACCAACTCC
CCGACTCTGATCGTTATGATTGGTTTGCCAGCCCGGGGTAAAACCTACGT
GTCCAAGAAACTAACACGCTACCTCAACTGGATTGGAGTCCCCACCAAAG
TGTTTAATCTTGGGGTGTATCGGCGTGAAGCAGTCAAGTCCTATAAGTCC
TACGACTTCTTTCGGCATGACAATGAGGAGGCCATGAAGATCCGCAAACA
GTGTGCTCTGGTGGCGCTGGAAGATGTTAAGGCATATCTCACTGAGGAGA
ATGGTCAGATTGCGGTGTTTGATGCCACCAATACAACCCGGGAGAGGAGG
GACATGATTTTGAACTTTGCTGAACAGGATTCCTTCAAGGTATTCTTTGT
GGAATCCGTCTGTGATGATCCTGATGTCATTGCTGCCAATATTCTGGAGG
TTAAGGTATCAAGCCCTGACTATCCTGAAAGGAACAGAGAGAACGTGATG
GAGGACTTCCTGAAGAGAATTGAATGCTACAAAGTTACCTACCGACCTCT
TGACCCAGACAACTATGACAAGGATCTTTCTTTCATCAAGGTGATAAACG
TGGGCCAGCGATTTTTAGTCAACAGAGTCCAGGACTACATCCAGAGCAAG
ATAGTCTACTACCTCATGAATATCCACGTCCAGCCTCGCACCATTTACCT
TTGCCGGCATGGAGAAAGCGAGTTCAATCTCTTGGGGAAGATTGGGGGTG
ACTCTGGCCTCTCGGTGCGGGGAAAGCAGTTTGCCCAAGCTCTAAGGAAA
TTTCTGGAGGAACAGGAAATAACAGACCTCAAAGTGTGGACAAGCCAGTT
GAAGAGGACCATACAGACTGCTGAATCTCTCGGGGTGCCCTATGAGCAGT
GGAAGATTCTGAATGAGATTGATGCTGGTGTGTGTGAAGAGATGACCTAT
GCAGAGATTGAGAAACGGTACCCAGAAGAGTTTGCACTTCGAGATCAAGA
GAAGTATCTGTATCGATATCCTGGTGGGGAGTCATACCAGGACCTGGTGC
AGCGGCTGGAGCCTGTCATCATGGAGCTGGAACGTCAGGGCAATGTCCTC
GTCATCTCCCACCAGGCTGTCATGCGCTGCCTCCTGGCCTACTTCTTGGA
TAAGGGCGCAGATGAGCTACCATACTTGAGATGCCCTCTCCATACCATCT
TCAAACTTACTCCTGTGGCCTATGGGTGCAAAGTGGAAACAATTAAACTT
AACGTGGAGGCTGTGAACACGCACCGTGACAAGCCAACTAACAACTTCCC
CAAGAACCAAACCCCTGTAAGGATGAGAAGGAACAGCTTTACGCCTCTGT
CCAGTTCGAATACAATAAGGCGTCCAAGAAATTACGCTGTTGGGAGCCGG
CCCCTCAAGCCCCTCAGCCCTCTCCGTGCCCAGGACATGCAAGAAGGGGC
CGACTAGGGATCC

Amino Acid Sequence

MYPYDVPDYASGASSSEQNNNSYETKTPNLRMSEKKCSWASYMTNSPTLI
VMIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYKSYDFF
RHDNEEAMKIRKQCALVALEDVKAYLTEENGQIAVFDATNTTRERRDMIL
NFAEQDSFKVFFVESVCDDPDVIAANILEVKVSSPDYPERNRENVMEDFL
KRIECYKVTYRPLDPDNYDKDLSFIKVINVGQRFLVNRVQDYIQSKIVYY
LMNIHVQPRTIYLCRHGESEFNLLGKIGGDSGLSVRGKQFAQALRKFLEE
QEITDLKVWTSQLKRTIQTAESLGVPYEQWKILNEIDAGVCEEMTYAEIE
KRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNVLVISH
QAVMRCLLAYFLDKGADELPYLRCPLHTIFKLTPVAYGCKVETIKLNVEA
VNTHRDKPTNNFPKNQTPVRMRRNSFTPLSSSNTIRRPRNYAVGSRPLKP
LSPLRAQDMQEGAD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31253
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_006212.2 CDS:; g342a (silent), a436g (N146D); N terminal HA tag for mammalian expression; ; Estimated MW of expressed protein : 59456 Daltons