cDNA Clone PTPN1

• Plasmid: pACYCDuet PTPN1
• Parent Plasmid: pACYC Duet1
• Expressed: 6His-PTPN1
• Genbank: NM_002827.2 GI:18104977
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGATGGAGATGGAAAAGGAGTTCGAGCAGATCGACAAGTCCGGGAG
CTGGGCGGCCATTTACCAGGATATCCGACATGAAGCCAGTGACTTCCCAT
GTAGAGTGGCCAAGCTTCCTAAGAACAAAAACCGAAATAGGTACAGAGAC
GTCAGTCCCTTTGACCATAGTCGGATTAAACTACATCAAGAAGATAATGA
CTATATCAACGCTAGTTTGATAAAAATGGAAGAAGCCCAAAGGAGTTACA
TTCTTACCCAGGGCCCTTTGCCTAACACATGCGGTCACTTTTGGGAGATG
GTGTGGGAGCAGAAAAGCAGGGGTGTCGTCATGCTCAACAGAGTGATGGA
GAAAGGTTCGTTAAAATGCGCACAATACTGGCCACAAAAAGAAGAAAAAG
AGATGATCTTTGAAGACACAAATTTGAAATTAACATTGATCTCTGAAGAT
ATCAAGTCATATTATACAGTGCGACAGCTAGAATTGGAAAACCTTACAAC
CCAAGAAACTCGAGAGATCTTACATTTCCACTATACCACATGGCCTGACT
TTGGAGTCCCTGAATCACCAGCCTCATTCTTGAACTTTCTTTTCAAAGTC
CGAGAGTCAGGGTCACTCAGCCCGGAGCACGGGCCCGTTGTGGTGCACTG
CAGTGCAGGCATCGGCAGGTCTGGAACCTTCTGTCTGGCTGATACCTGCC
TCTTGCTGATGGACAAGAGGAAAGACCCTTCTTCCGTTGATATCAAGAAA
GTGCTGTTAGAAATGAGGAAGTTTCGGATGGGGCTGATCCAGACAGCCGA
CCAGCTGCGCTTCTCCTACCTGGCTGTGATCGAAGGTGCCAAATTCATCA
TGGGGGACTCTTCCGTGCAGGATCAGTGGAAGGAGCTTTCCCACGAGGAC
CTGGAGCCCCCACCCGAGCATATCCCCCCACCTCCCCGGCCACCCAAACG
AATCCTGGAGCCACACAATGGGAAATGCAGGGAGTTCTTCCCAAATCACC
AGTGGGTGAAGGAAGAGACCCAGGAGGATAAAGACTGCCCCATCAAGGAA
GAAAAAGGAAGCCCCTTAAATGCCGCACCCTACGGCATCGAAAGCATGAG
TCAAGACACTGAAGTTAGAAGTCGGGTCGTGGGGGGAAGTCTTCGAGGTG
CCCAGGCTGCCTCCCCAGCCAAAGGGGAGCCGTCACTGCCCGAGAAGGAC
GAGGACCATGCACTGAGTTACTGGAAGCCCTTCCTGGTCAACATGTGCGT
GGCTACGGTCCTCACGGCCGGCGCTTACCTCTGCTACAGGTTCCTGTTCA
ACAGCAACACATAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSQDPMEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAK
LPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQG
PLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFE
DTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPE
SPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMD
KRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSS
VQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKE
ETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAAS
PAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT*

• Vector Type: Bacterial
• Antibiotic: Chloramphenicol
• Full Snapgene Map: pACYCDuet PTPN145.54 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU31201
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No