cDNA Clone TrkA

• Plasmid: pcDNA5-FRT/TO TrkA
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: TrkA (untagged)
• Genbank: NM_001007792.1 GI:56118209*
• Tag: untagged
• Species: Human

Sequence of Insert

GCGGCCGCGATGAAGGAGGCCGCCCTCATCTGCCTGGCACCCTCTGTACC
CCCGATCTTGACGGTGAAGTCCTGGGACACCATGCAGTTGCGGGCTGCTA
GATCTCGGTGCACAAACTTGTTGGCAGCAAGCTACATCGAGAACCAGCAG
CATCTGCAGCATCTGGAGCTCCGTGATCTGAGGGGCCTGGGGGAGCTGAG
AAACCTCACCATCGTGAAGAGTGGTCTCCGTTTCGTGGCGCCAGATGCCT
TCCATTTCACTCCTCGGCTCAGTCGCCTGAATCTCTCCTTCAACGCTCTG
GAGTCTCTCTCCTGGAAAACTGTGCAGGGCCTCTCCTTACAGGAACTGGT
CCTGTCGGGGAACCCTCTGCACTGTTCTTGTGCCCTGCGCTGGCTACAGC
GCTGGGAGGAGGAGGGACTGGGCGGAGTGCCTGAACAGAAGCTGCAGTGT
CATGGGCAAGGGCCCCTGGCCCACATGCCCAATGCCAGCTGTGGTGTGCC
CACGCTGAAGGTCCAGGTGCCCAATGCCTCGGTGGATGTGGGGGACGACG
TGCTGCTGCGGTGCCAGGTGGAGGGGCGGGGCCTGGAGCAGGCCGGCTGG
ATCCTCACAGAGCTGGAGCAGTCAGCCACGGTGATGAAATCTGGGGGTCT
GCCATCCCTGGGGCTGACCCTGGCCAATGTCACCAGTGACCTCAACAGGA
AGAACGTGACGTGCTGGGCAGAGAACGATGTGGGCCGGGCAGAGGTCTCT
GTTCAGGTCAACGTCTCCTTCCCGGCCAGTGTGCAGCTGCACACGGCGGT
GGAGATGCACCACTGGTGCATCCCCTTCTCTGTGGATGGGCAGCCGGCAC
CGTCTCTGCGCTGGCTCTTCAATGGCTCCGTGCTCAATGAGACCAGCTTC
ATCTTCACTGAGTTCCTGGAGCCGGCAGCCAATGAGACCGTGCGGCACGG
GTGTCTGCGCCTCAACCAGCCCACCCACGTCAACAACGGCAACTACACGC
TGCTGGCTGCCAACCCCTTCGGCCAGGCCTCCGCCTCCATCATGGCTGCC
TTCATGGACAACCCTTTCGAGTTCAACCCCGAGGACCCCATCCCTGTCTC
CTTCTCGCCGGTGGACACTAACAGCACATCTGGAGACCCGGTGGAGAAGA
AGGACGAAACACCTTTTGGGGTCTCGGTGGCTGTGGGCCTGGCCGTCTTT
GCCTGCCTCTTCCTTTCTACGCTGCTCCTTGTGCTCAACAAATGTGGACG
GAGAAACAAGTTTGGGATCAACCGCCCGGCTGTGCTGGCTCCAGAGGATG
GGCTGGCCATGTCCCTGCATTTCATGACATTGGGTGGCAGCTCCCTGTCC
CCCACCGAGGGCAAAGGCTCTGGGCTCCAAGGCCACATCATCGAGAACCC
ACAATACTTCAGTGATGCCTGTGTTCACCACATCAAGCGCCGGGACATCG
TGCTCAAGTGGGAGCTGGGGGAGGGCGCCTTTGGGAAGGTCTTCCTTGCT
GAGTGCCACAACCTCCTGCCTGAGCAGGACAAGATGCTGGTGGCTGTCAA
GGCACTGAAGGAGGCGTCCGAGAGTGCTCGGCAGGACTTCCAGCGTGAGG
CTGAGCTGCTCACCATGCTGCAGCACCAGCACATCGTGCGCTTCTTCGGC
GTCTGCACCGAGGGCCGCCCCCTGCTCATGGTCTTTGAGTATATGCGGCA
CGGGGACCTCAACCGCTTCCTCCGATCCCATGGACCTGATGCCAAGCTGC
TGGCTGGTGGGGAGGATGTGGCTCCAGGCCCCCTGGGTCTGGGGCAGCTG
CTGGCCGTGGCTAGCCAGGTCGCTGCGGGGATGGTGTACCTGGCGGGTCT
GCATTTTGTGCACCGGGACCTGGCCACACGCAACTGTCTAGTGGGCCAGG
GACTGGTGGTCAAGATTGGTGATTTTGGCATGAGCAGGGATATCTACAGC
ACCGACTATTACCGTGTGGGAGGCCGCACCATGCTGCCCATTCGCTGGAT
GCCGCCCGAGAGCATCCTGTACCGTAAGTTCACCACCGAGAGCGACGTGT
GGAGCTTCGGCGTGGTGCTCTGGGAGATCTTCACCTACGGCAAGCAGCCC
TGGTACCAGCTCTCCAACACGGAGGCAATCGACTGCATCACGCAGGGACG
TGAGTTGGAGCGGCCACGTGCCTGCCCACCAGAGGTCTACGCCATCATGC
GGGGCTGCTGGCAGCGGGAGCCCCAGCAACGCCACAGCATCAAGGATGTG
CACGCCCGGCTGCAAGCCCTGGCCCAGGCACCTCCTGTCTACCTGGATGT
CCTGGGCTAGCGGCCGC

Amino Acid Sequence

MKEAALICLAPSVPPILTVKSWDTMQLRAARSRCTNLLAASYIENQQHLQ
HLELRDLRGLGELRNLTIVKSGLRFVAPDAFHFTPRLSRLNLSFNALESL
SWKTVQGLSLQELVLSGNPLHCSCALRWLQRWEEEGLGGVPEQKLQCHGQ
GPLAHMPNASCGVPTLKVQVPNASVDVGDDVLLRCQVEGRGLEQAGWILT
ELEQSATVMKSGGLPSLGLTLANVTSDLNRKNVTCWAENDVGRAEVSVQV
NVSFPASVQLHTAVEMHHWCIPFSVDGQPAPSLRWLFNGSVLNETSFIFT
EFLEPAANETVRHGCLRLNQPTHVNNGNYTLLAANPFGQASASIMAAFMD
NPFEFNPEDPIPVSFSPVDTNSTSGDPVEKKDETPFGVSVAVGLAVFACL
FLSTLLLVLNKCGRRNKFGINRPAVLAPEDGLAMSLHFMTLGGSSLSPTE
GKGSGLQGHIIENPQYFSDACVHHIKRRDIVLKWELGEGAFGKVFLAECH
NLLPEQDKMLVAVKALKEASESARQDFQREAELLTMLQHQHIVRFFGVCT
EGRPLLMVFEYMRHGDLNRFLRSHGPDAKLLAGGEDVAPGPLGLGQLLAV
ASQVAAGMVYLAGLHFVHRDLATRNCLVGQGLVVKIGDFGMSRDIYSTDY
YRVGGRTMLPIRWMPPESILYRKFTTESDVWSFGVVLWEIFTYGKQPWYQ
LSNTEAIDCITQGRELERPRACPPEVYAIMRGCWQREPQQRHSIKDVHAR
LQALAQAPPVYLDVLG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31182
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_001007792.1 CDS:; Insertion of GTCTCCTTCTCGCCGGTG after nt 1086 (insertion of residues VSFSPV after P362)