cDNA Clone TH

• Plasmid: pcDNA5-FRT/TO-GFP TH isoform b S19A
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP TH isoform b S19A
• Genbank: NM_000360.3 GI:88900502*
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGCCCACCCCCGACGCCACCACGCCACAGGCCAAGGGCTTCCG
CAGGGCCGTGGCTGAGCTGGACGCCAAGCAGGCAGAGGCCATCATGTCCC
CGCGGTTCATTGGGCGCAGGCAGAGCCTCATCGAGGACGCCCGCAAGGAG
CGGGAGGCGGCGGTGGCAGCAGCGGCCGCTGCAGTCCCCTCGGAGCCCGG
GGACCCCCTGGAGGCTGTGGCCTTTGAGGAGAAGGAGGGGAAGGCCATGC
TAAACCTGCTCTTCTCCCCGAGGGCCACCAAGCCCTCGGCGCTGTCCCGA
GCTGTGAAGGTGTTTGAGACGTTTGAAGCCAAAATCCACCATCTAGAGAC
CCGGCCCGCCCAGAGGCCGCGAGCTGGGGGCCCCCACCTGGAGTACTTCG
TGCGCCTCGAGGTGCGCCGAGGGGACCTGGCCGCCCTGCTCAGTGGTGTG
CGCCAGGTGTCAGAGGACGTGCGCAGCCCCGCGGGGCCCAAGGTCCCCTG
GTTCCCAAGAAAAGTGTCAGAGCTGGACAAGTGTCATCACCTGGTCACCA
AGTTCGACCCTGACCTGGACTTGGACCACCCGGGCTTCTCGGACCAGGTG
TACCGCCAGCGCAGGAAGCTGATTGCTGAGATCGCCTTCCAGTACAGGCA
CGGCGACCCGATTCCCCGTGTGGAGTACACCGCCGAGGAGATTGCCACCT
GGAAGGAGGTCTACACCACGCTGAAGGGCCTCTACGCCACGCACGCCTGC
GGGGAGCACCTGGAGGCCTTTGCTTTGCTGGAGCGCTTCAGCGGCTACCG
GGAAGACAATATCCCCCAGCTGGAGGACGTCTCCCGCTTCCTGAAGGAGC
GCACGGGCTTCCAGCTGCGGCCTGTGGCCGGCCTGCTGTCCGCCCGGGAC
TTCCTGGCCAGCCTGGCCTTCCGCGTGTTCCAGTGCACCCAGTATATCCG
CCACGCGTCCTCGCCCATGCACTCCCCTGAGCCGGACTGCTGCCACGAGC
TGCTGGGGCACGTGCCCATGCTGGCCGACCGCACCTTCGCGCAGTTCTCG
CAGGACATTGGCCTGGCGTCCCTGGGGGCCTCGGATGAGGAAATTGAGAA
GCTGTCCACGCTGTACTGGTTCACGGTGGAGTTCGGGCTGTGTAAGCAGA
ACGGGGAGGTGAAGGCCTATGGTGCCGGGCTGCTGTCCTCCTACGGGGAG
CTCCTGCACTGCCTGTCTGAGGAGCCTGAGATTCGGGCCTTCGACCCTGA
GGCTGCGGCCGTGCAGCCCTACCAAGACCAGACGTACCAGTCAGTCTACT
TCGTGTCTGAGAGCTTCAGTGACGCCAAGGACAAGCTCAGGAGCTATGCC
TCACGCATCCAGCGCCCCTTCTCCGTGAAGTTCGACCCGTACACGCTGGC
CATCGACGTGCTGGACAGCCCCCAGGCCGTGCGGCGCTCCCTGGAGGGTG
TCCAGGATGAGCTGGACACCCTTGCCCATGCGCTGAGTGCCATTGGCTAG
GATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPTPDA
TTPQAKGFRRAVAELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAA
AAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETF
EAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVR
SPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLI
AEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFA
LLERFSGYREDNIPQLEDVSRFLKERTGFQLRPVAGLLSARDFLASLAFR
VFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASL
GASDEEIEKLSTLYWFTVEFGLCKQNGEVKAYGAGLLSSYGELLHCLSEE
PEIRAFDPEAAAVQPYQDQTYQSVYFVSESFSDAKDKLRSYASRIQRPFS
VKFDPYTLAIDVLDSPQAVRRSLEGVQDELDTLAHALSAIG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31132
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_000360.3 GI:88900502 CDS:; g241a (V81M) - present in other published sequences.