cDNA Clone slit-robo-GAP 1

• Plasmid: pcDNA5-FRT/TO-GFP slit-robo-GAP 1 isoform 2 D843-end
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP srGAP1-2 D843-end
• Genbank: AB037725*
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccgatggccattgcccgctccaccctccacatgccctttctaactcctcagttgacctagggtccccaagccttgccagtcacccccggggcctgctgcagaaccgtggcctcaacaatgacagtcctgagcggaggcgcaggcctggccatggcagcctgaccaacatcagccggcacgactccctcaagaagatcgacagccctcccattagaaggtccacgtcatcagggcaatacacgggcttcaatgaccacaagccactggacccagagacaattgctcaggatattgaagaaacg
atgaacacagctttgaatgaactccgagaactggagagacagagcacagc
aaagcatgcccctgatgtggtgctggataccctggagcaagtgaaaaact
ctcccacccctgccacttccacggaatctctcagccctttgcacaacgtt
gccctcaggagctccgagcctcagattcgacgtagcacgagctcctccag
tgacacaatgagtactttcaagcctatggtggcacccagaatgggcgtgc
agctgaagcctccagcccttaggccaaaacctgctgttcttccaaaaaca
aatcctaccataggacctgccccacctccccagggtccaacagacaagtc
atgcacaatgtaagcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSDGHCPL
HPPHALSNSSVDLGSPSLASHPRGLLQNRGLNNDSPERRRRPGHGSLTNI
SRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALN
ELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSE
PQIRRSTSSSSDTMSTFKPMVAPRMGVQLKPPALRPKPAVLPKTNPTIGP
APPPQGPTDKSCTM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31109
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Start codon not identified in AB037725. Numbering for cloned full length srGAP 1-2 starts as isoform 1 (NM_020762) starts, i.e. MSTPSRFKKDKEIIAEYESQVKEIR… See DU5909