cDNA Clone slit-robo-GAP 2

• Plasmid: pcDNA5-FRT/TO-FLAG srGAP2a D89-end
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG srGAP2a D89-end
• Genbank: NM_015326
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTGATCAGAATGTTCTCTCTCCAGTCAACTGCTGGAATCTCCTCTT
AAACCAGGTGAAGCGGGAAAGCAGGGACCATACCACCCTGAGTGACATCT
ACCTGAATAATATCATTCCTCGATTTGTACAAGTCAGCGAGGACTCAGGA
AGACTCTTTAAAAAGAGTAAAGAAGTCGGCCAGCAGCTCCAAGATGATTT
GATGAAGGTCCTGAACGAGCTCTACTCGGTCATGAAGACATATCACATGT
ACAATGCCGACAGCATCAGTGCTCAGAGCAAACTAAAGGAGGCGGAGAAG
CAGGAGGAGAAGCAAATTGGTAAATCGGTAAAGCAGGAGGACCGGCAGAC
CCCACGCTCCCCTGACTCCACGGCCAATGTTCGCATTGAGGAGAAACATG
TCCGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGAAGCGCCAA
GCCAAGTACACGGAGAATAAGCTGAAGGCCATCAAAGCCCGGAATGAGTA
CTTGCTGGCTTTGGAGGCAACCAATGCATCTGTCTTCAAGTACTACATCC
ATGACCTATCTGACCTTATTGATCAGTGTTGTGACTTAGGCTACCATGCA
AGTCTGAACCGGGCTCTACGCACCTTCCTCTCTGCTGAGTTAAACCTGGA
ACAGTCGAAGCATGAGGGTCTGGATGCCATCGAGAATGCAGTAGAAAACC
TGGATGCCACCAGTGACAAGCAGCGCCTCATGGAGATGTACAACAACGTC
TTCTGCCCCCCTATGAAGTTTGAGTTTCAGCCCCACATGGGGGATATGGC
TTCCCAGCTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTACAGAGAT
GCCAACAACTGCAGTCTCGCTTATCCACTCTAAAGATTGAAAACGAAGAG
GTAAAGAAGACAATGGAGGCCACCCTGCAAACCATCCAGGACATTGTGAC
TGTCGAGGACTTTGATGTGTCTGACTGCTTCCAGTACAGCAACTCCATGG
AGTCCGTCAAGTCCACGGTCTCTGAAACCTTCATGAGCAAGCCCAGCATT
GCTAAGAGGAGAGCCAACCAGCAAGAGACAGAGCAGTTTTATTTCACAAA
AATGAAAGAGTACCTGGAGGGCAGGAACCTCATCACCAAGTTACAAGCCA
AGCATGACCTTCTGCAGAAAACCCTGGGAGAAAGTCAGCGGACAGATTGC
AGTCTAGCCAGGCGCAGCTCAACTGTGAGGAAACAGGACTCCAGCCAGGC
AATTCCTCTAGTGGTGGAAAGCTGTATCCGGTTTATCAGCAGACACGGAC
TACAGCATGAAGGAATTTTCCGGGTGTCAGGATCCCAGGTGGAAGTGAAT
GACATCAAAAATGCCTTTGAGAGAGGAGAGGACCCCCTGGCTGGGGACCA
GAACGACCATGACATGGATTCCATAGCTGGTGTCCTGAAGCTTTACTTCC
GGGGGCTGGAACACCCTCTCTTCCCCAAGGACATCTTTCATGACCTGATG
GCCTGCGTCACAATGGACAACCTGCAGGAGAGAGCTCTGCACATCCGGAA
AGTCCTCCTAGTCCTGCCCAAGACCACTCTGATTATCATGAGATACCTCT
TTGCCTTCCTCAATCATTTATCACAGTTCAGTGAAGAGAACATGATGGAC
CCCTACAACCTCGCCATCTGCTTCGGGCCCTCGCTAATGTCAGTGCCAGA
GGGCCACGACCAGGTGTCCTGCCAAGCCCACGTGAATGAGCTGATCAAAA
CCATCATCATCCAGCATGAGAACATCTTCCCAAGCCCCAGGGAGCTGGAG
GGCCCTGTCTACAGCAGAGGAGGAAGCATGGAGGATTACTGTGATAGCCC
TCATGGAGAGACTACCTCGGTTGAAGACTCAACCCAGGATGTGACCGCAG
AGCACCACACGAGCGATGACGAATGTGAGCCCATCGAGGCCATTGCCAAG
TTTGACTACGTGGGCCGGACAGCCCGAGAGCTGTCCTTTAAGAAGGGAGC
ATCCCTGCTGCTTTACCAGCGGGCTTCCGACGACTGGTGGGAAGGCCGGC
ACAATGGCATCGACGGACTCATCCCCCATCAGTACATCGTGGTCCAAGAC
ACCGAGGACGGTGTCGTGGAGAGGTCCAGCCCCAAGTCTGAGATTGAGGT
CATTTCTGAGCCACCTGAAGAAAAGGTGACAGCCAGAGCGGGGGCCAGCT
GTCCCAGTGGGGGTCATGTAGCCGATATTTATCTTGCAAACATCAACAAG
CAAAGGAAGCGTCCAGAATCTGGGAGCATCCGGAAAACTTTTCGGAGTGA
CAGCCACGGGCTGAGCAGTTCCCTGACTGACTCCTCCTCCCCAGGGGTGG
GGGCTAGCTGCCGCCCATCCTCCCAGCCCATCATGAGCCAGAGCCTCCCC
AAAGAAGGGCCAGATAAGTGTTCCATCAGTGGGCACGGGAGCCTCAACTC
CATCAGCCGCCACTCATCCCTGAAGAATCGGCTGGATAGTCCACAGATCC
GGAAGACTGCCACAGCGGGAAGGTCAAAAAGCTTCAATAACCATCGGCCC
ATGGACCCTGAGGTCATTGCTCAGGATATTGAGGCAACAATGAACTCGGC
CCTGAATGAGCTACGGGAACTAGAACGGCAGAGCAGTGTCAAACACACCC
CTGACGTGGTTCTGGACACCTTGGAGCCCCTCAAAACCTCCCCAGTGGTG
GCCCCCACGTCAGAGCCCTCCAGCCCTCTGCACACCCAGCTCCTCAAGGA
CCCCGAGCCCGCCTTCCAGCGCAGCGCCAGTACTGCTGGGGACATCGCCT
GCGCCTTCCGGCCTGTCAAGTCTGTCAAGATGGCTGCCCCGGTCAAACCA
CCAGCCACACGGCCCAAGCCCACTGTCTTCCCCAAAACAAATGCCACTAG
CCCTGGTGTCAACTCATCAACTTCCCCACAGTCTACTGACAAGTCTTGTA
CTGTCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIYLNNIIPR
FVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISA
QSKLKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKK
IEKMKEKRQAKYTENKLKAIKARNEYLLALEATNASVFKYYIHDLSDLID
QCCDLGYHASLNRALRTFLSAELNLEQSKHEGLDAIENAVENLDATSDKQ
RLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQPVQSELVQRCQQLQSRL
STLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSMESVKSTVS
ETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKT
LGESQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFR
VSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLF
PKDIFHDLMACVTMDNLQERALHIRKVLLVLPKTTLIIMRYLFAFLNHLS
QFSEENMMDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHEN
IFPSPRELEGPVYSRGGSMEDYCDSPHGETTSVEDSTQDVTAEHHTSDDE
CEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLI
PHQYIVVQDTEDGVVERSSPKSEIEVISEPPEEKVTARAGASCPSGGHVA
DIYLANINKQRKRPESGSIRKTFRSDSHGLSSSLTDSSSPGVGASCRPSS
QPIMSQSLPKEGPDKCSISGHGSLNSISRHSSLKNRLDSPQIRKTATAGR
SKSFNNHRPMDPEVIAQDIEATMNSALNELRELERQSSVKHTPDVVLDTL
EPLKTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIACAFRPVKS
VKMAAPVKPPATRPKPTVFPKTNATSPGVNSSTSPQSTDKSCTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16973
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee