cDNA Clone slit-robo-GAP 1

• Plasmid: pCMV5-HA slit-robo-GAP 1 isoform 2 1-890
• Parent Plasmid: pCMV5 HA1
• Expressed: HA srGAP1-2 1-890
• Genbank: AB037725*
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGTCCACCCCGAGCCGATTCAAGAAGGACAAAGAGATCATAGC
CGAGTATGAAAGTCAAGTCAAagaaattcgagctcaactggtagaacaac
aaaaatgcctggagcagcaaacggagatgcgagttcagcttctccaggat
ctgcaagatttcttccgaaaaaaagctgaaattgagacggaatattcccg
gaatctagagaagttagcagaaaggttcatggcaaaaacaagaagcacta
aggatcatcaacaatacaagaaagaccagaacctgttgtctccagtgaac
tgctggtatttgctcctgaaccaagtaaggagagaaagcaaagaccatgc
aaccttgagtgacatctatctgaacaatgtgattatgcggttcatgcaga
taagtgaggattctaccaggatgtttaaaaagagcaaagagattgcattc
caacttcatgaggatttaatgaaggttcttaatgagctttatacggtgat
gaaaacataccatatgtatcatgcagagagcatcagtgcagagagcaagc
tgaaagaggccgaaaaacaagaggaaaagcaaattgggagatctggtgat
ccagtcttccatattcgactagaggagagacatcaacggcgaagctctgt
aaagaaaattgaaaaaatgaaagaaaaaagacaagcaaaatattcagaaa
ataagctaaaatcaattaaggcacggaacgaatatctcctaacacttgaa
gccaccaatgcctcagttttcaagtactatattcatgatctttctgattt
aattgattgctgtgatcttggctaccatgcaagtctgaacagagccctaa
gaacatatctgtctgcggagtacaaccttgaaacctccagacatgagggc
ttagacattattgagaatgcagttgataatttagagcccaggagcgataa
gcagagattcatggagatgtaccctgctgcgttctgtccaccaatgaagt
ttgagtttcagtctcacatgggtgatgaggtgtgccaggtcagtgcccag
cagccagtccaggcagagctcatgctcaggtaccaacagttgcagtcccg
ccttgccacgctcaaaatcgagaatgaagaggttaagaaaacgactgaag
ccaccttgcagacgatacaagatatggtcaccatcgaggactatgatgtt
tctgaatgcttccagcacagtcgttccacagaatctgtgaagtccactgt
ctctgaaacctacctgagtaaacccagcatcgccaagagaagagccaacc
agcaggaaactgaacagttctacttcatgaaactcagagaatatttggaa
ggcagtaatctcatcacaaaacttcaagccaaacatgacttgctgcagag
gaccctgggagaaggtcatagagctgaatatatgactacaagccgaggac
gaagaaactcgcacacaagacatcaggactcaggacaggttattcccctc
attgtggaaagctgtattcggttcatcaatctctatggtcttcagcatca
ggggattttcagagtgtctggttcccaggtggaagtcaatgatattaaaa
attcatttgagagaggtgaaaatcctttggctgatgaccagagtaaccat
gatattaactcagttgctggcgttctgaagctctatttccgtgggctgga
aaaccccctctttcctaaggaaagatttaacgatctgatttcttgtatca
gaatagataatctctatgagagggcgcttcacatccgcaaactcctcctg
actttgcccaggtcggtccttatagtgatgaggtacctctttgccttcct
caatcatctatcacagtacagcgatgagaatatgatggacccttataacc
tggccatttgctttggcccaacattgatgcctgtcccagaaatacaggat
caagtgtcttgccaggcacatgtgaatgaaattatcaaaaccatcatcat
ccaccatgagactattttcccagatgctaaagagctggatggccctgttt
atgagaaatgtatggctggagatgactattgcgacagcccatacagtgag
cacggtacattggaggaagtggaccaagatgctggtacagagccccacac
aagtgaagatgaatgtgagccaatagaagcaatagccaagtttgactatg
ttgggcggtccgccagagaactatccttcaagaagggtgcctccctgctg
ctgtatcaccgtgcatctgaggactggtgggaaggcaggcacaacgggat
tgacgggctggtgcctcaccagtatatagtggtgcaggatatggatgata
cgttttcagacactctgagccaaaaagccgacagtgaggccagcagtggg
ccagtcacggaagacaagtcctcatccaaggacatgaactccccgacaga
ccgtcatcctgacggctatttagccaggcaacgaaaaagaggagagccac
cccctccagtaaggcgtcctggcaggaccagtgatggccattgcccgctc
caccctccacatgccctttctaactcctcagttgacctagggtccccaag
ccttgccagtcacccccggggcctgctgcagaaccgtggcctcaacaatg
acagtcctgagcggaggcgcaggcctTAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQ
TEMRVQLLQDLQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQYK
KDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTR
MFKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQ
EEKQIGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIK
ARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAE
YNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHM
GDEVCQVSAQQPVQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQ
DMVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQF
YFMKLREYLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTTSRGRRNSHTR
HQDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGE
NPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYE
RALHIRKLLLTLPRSVLIVMRYLFAFLNHLSQYSDENMMDPYNLAICFGP
TLMPVPEIQDQVSCQAHVNEIIKTIIIHHETIFPDAKELDGPVYEKCMAG
DDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARE
LSFKKGASLLLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLS
QKADSEASSGPVTEDKSSSKDMNSPTDRHPDGYLARQRKRGEPPPPVRRP
GRTSDGHCPLHPPHALSNSSVDLGSPSLASHPRGLLQNRGLNNDSPERRR
RP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16664
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Start codon not identified in AB037725. This clone starts as isoform 1 (NM_020762) starts, i.e. MSTPSRFKKDKEIIAEYESQVKEIR…; ; Estimated MW of expressed protein : 104345 Daltons