cDNA Clone SASH1

• Plasmid: pcDNA5-FRT/TO-GFP SASH1 Q715-M1247(end)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP SASH1 Q715-M1247(end)
• Genbank: NM_015278.3 GI:45935384*
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCTACATGGCCTCAGGGCCTGAGTGGATGCTCACCCCGAGACTC
AGGATGCTACGAAAGCAGTGAGAACCTGGAAAACGGCAAGACTCGGAAAG
CTAGCCTCCTATCTGCCAAGTCATCCACCGAGCCCAGCTTGAAGTCTTTT
AGCAGAAACCAGTTGGGCAATTACCCAACATTGCCTTTAATGAAATCAGG
GGATGCACTGAAGCAGGGACAGGAGGAGGGCAGGCTGGGTGGTGGCCTTG
CCCCAGACACGTCCAAGAGCTGTGACCCACCTGGTGTGACTGGTTTGAAT
AAAAACCGAAGAAGCCTCCCAGTTTCCATCTGCCGGAGCTGTGAGACCCT
GGAGGGCCCCCAGACTGTGGACACTTGGCCCCGATCCCATTCCCTGGATG
ACCTTCAAGTGGAGCCTGGTGCTGAGCAAGACGTGCCTACCGAGGTGACA
GAACCGCCCCCTCAGATTGTACCTGAAGTGCCACAGAAGACGACCGCCTC
TTCCACGAAGGCCCAGCCCCTGGAGCGAGACTCTGCTGTCGACAATGCAT
TGCTACTGACCCAAAGCAAGAGATTTTCTGAACCTCAGAAATTGACAACT
AAGAAACTGGAGGGCTCAATCGCAGCCTCTGGTCGCGGCCTGTCACCCCC
TCAGTGTTTGCCCAGAAACTATGATGCTCAGCCTCCTGGAGCTAAACACG
GTTTAGCAAGGACGCCTCTGGAGGGCCACAGAAAAGGACACGAGTTTGAA
GGAACACACCATCCCCTGGGCACCAAAGAAGGGGTAGATGCTGAGCAGAG
AATGCAGCCCAAAATTCCATCACAGCCTCCACCTGTTCCTGCCAAAAAGA
GCAGAGAACGCCTTGCTAACGGACTCCACCCTGTTCCCATGGGCCCCAGT
GGGGCCCTCCCCAGTCCCGATGCGCCATGCCTGCCAGTGAAAAGGGGCAG
CCCCGCCAGCCCCACCAGCCCTAGCGACTGTCCCCCAGCACTGGCTCCCA
GGCCTCTCTCAGGGCAGGCGCCTGGCAGCCCACCAAGCACAAGGCCGCCC
CCCTGGCTCTCAGAGCTCCCCGAGAACACAAGCCTCCAGGAGCACGGTGT
GAAGCTGGGCCCGGCTTTGACCAGGAAGGTCTCCTGTGCCCGGGGAGTGG
ATCTAGAAACGCTCACTGAAAACAAGCTGCACGCTGAAGGCATCGATCTC
ACGGAGGAGCCGTATTCTGATAAGCATGGCCGCTGTGGGATTCCTGAAGC
CCTGGTGCAGAGATACGCAGAGGACTTGGATCAGCCCGAGCGGGACGTCG
CCGCCAACATGGACCAGATCCGGGTGAAGCAGCTTCGGAAGCAGCACCGC
ATGGCGATTCCAAGTGGTGGACTCACGGAAATCTGCCGAAAGCCCGTCTC
TCCTGGGTGCATTTCGTCTGTGTCAGATTGGCTCATTTCCATCGGTCTGC
CCATGTACGCCGGCACCCTCTCCACCGCGGGCTTCAGCACACTGAGCCAA
GTGCCTTCTCTGTCTCACACTTGCCTTCAGGAGGCCGGCATCACAGAGGA
GAGACACATAAGAAAGCTCCTATCTGCAGCCAGACTCTTCAAACTGCCGC
CAGGCCCTGAGGCCATGTAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAATWPQGLSGCSPRDSGCYESSENLENGKTRKASLLSAKSSTEPSLKS
FSRNQLGNYPTLPLMKSGDALKQGQEEGRLGGGLAPDTSKSCDPPGVTGL
NKNRRSLPVSICRSCETLEGPQTVDTWPRSHSLDDLQVEPGAEQDVPTEV
TEPPPQIVPEVPQKTTASSTKAQPLERDSAVDNALLLTQSKRFSEPQKLT
TKKLEGSIAASGRGLSPPQCLPRNYDAQPPGAKHGLARTPLEGHRKGHEF
EGTHHPLGTKEGVDAEQRMQPKIPSQPPPVPAKKSRERLANGLHPVPMGP
SGALPSPDAPCLPVKRGSPASPTSPSDCPPALAPRPLSGQAPGSPPSTRP
PPWLSELPENTSLQEHGVKLGPALTRKVSCARGVDLETLTENKLHAEGID
LTEEPYSDKHGRCGIPEALVQRYAEDLDQPERDVAANMDQIRVKQLRKQH
RMAIPSGGLTEICRKPVSPGCISSVSDWLISIGLPMYAGTLSTAGFSTLS
QVPSLSHTCLQEAGITEERHIRKLLSAARLFKLPPGPEAM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16661
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_015278.3 CDS:; a492g (sil), a632t (K211M) (Neither applicable in this clone) ; a2651g (Q884R); Coding diffs present in other published seqs (match AAH63279.1)