cDNA Clone SASH1

• Plasmid: pcDNA5-FRT/TO-GFP SASH1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP SASH1
• Genbank: NM_015278.3 GI:45935384*
• Tag: GFP
• Species: Human

Sequence of Insert

gcggccgcgatggaggacgcgggagcagctggcccggggccggagcctgagcccgagcccgagccggagcccgagcccgcgccggagccggaaccggagcccaagccgggtgctggcacatccgaggcgttctcccgactctggaccgacgtgatgggtatcctggacggttcactgggaaacatcgatgacctggcgcagcagtatgcagattattacaacacctgtttctccgacgtgtgcgagaggatggaggagctgcggaaacggcgggtttcccaggacctggaagtggagaaacccgatgctagccccacgtcacttcagctgcggtcccagatcgaagagtcgcttggcttctgtagcgccgtgtcaaccccagaagtggaaagaaagaaccctcttcataaatcaaactcagaagacagctctgtaggaaaaggagactggaagaagaaa
aataagtatttctggcagaacttccgaaagaaccagaaagggataatgag
acagacttcaaaaggagaagacgttggttatgttgccagtgaaataacga
tgagcgatgaggagcggattcagctaatgatgatggtcaaagaaaagatg
atcacaattgaggaagcacttgctaggctcatggaatacgaggcccagca
ccggcagtcggctgccctggaccctgctgactggccagatggttcttacc
caacgtttgatggctcatcaaactgcaattcaagagaacaatcggatgat
gagactgaggagtcggtgaagtttaagaggttacacaagctggtaaactc
cactcgcagagtcagaaagaaactaattagggtggaagaaatgaaaaaac
ccagcactgaaggtggggaggagcacgtgtttgagaattcgccggtcctg
gatgaacggtccgccctctactctggcgtgcacaagaagccccttttctt
tgatggctctcctgagaaacctcccgaagatgactcagactctctcacca
cgtctccatcctccagcagcctggacacctggggggctggccggaagttg
gtcaaaaccttcagcaaaggagagagccggggcctgattaagccccccaa
gaagatggggacattcttctcctacccagaagaagaaaaggcccagaaag
tgtcccgctccctcaccgagggggagatgaagaagggtctcgggtcccta
agccacgggagaacctgcagttttggaggatttgacttgacgaatcgctc
tctgcacgttggcagtaataattctgacccaatgggtaaagaaggagact
ttgtgtacaaagaagtcatcaaatcacctactgcctctcgcatctctctt
gggaaaaaggtgaaatcagtgaaagagacgatgagaaagagaatgtctaa
aaaatacagcagctctgtctctgagcaggactcgggccttgatggaatgc
ctggctcccctccgccttcacagcccgaccccgaacacttggacaagccc
aagctcaaggccgggggttctgtagaaagtcttcgcagttctctcagtgg
gcagagctccatgagcggtcaaacagtgagcaccactgattcctcaacca
gcaaccgggaaagcgtcaagtcggaagatggggatgacgaagagccgcct
taccgaggcccgttctgcgggcgtgccagggtgcacaccgacttcacccc
cagtccctatgacacagactcactcaagctcaagaaaggagatatcatcg
atataatcagcaagccacccatggggacctggatgggcctgctgaacaac
aaagtcggcacgttcaagttcatctacgtggacgtgctcagtgaagacga
ggagaaacccaaacgccccaccaggaggcgtcggaaaggacgaccacccc
agcccaagtctgtggaggatctcctggatcggattaacctaaaagagcac
atgcccactttcctgttcaatggatatgaagatttggacacctttaagct
gctggaggaggaagacttggatgagttaaatatcagggacccggaacaca
gagctgttctcttgacagcagtggagctgttacaagagtatgacagtaac
agcgaccagtcaggatcccaggagaagctgctcgttgacagccagggcct
gagtggatgctcaccccgagactcaggatgctacgaaagcagtgagaacc
tggaaaacggcaagactcggaaagctagcctcctatctgccaagtcatcc
accgagcccagcttgaagtcttttagcagaaaccagttgggcaattaccc
aacattgcctttaatgaaatcaggggatgcactgaagcagggacaggagg
agggcaggctgggtggtggccttgccccagacacgtccaagagctgtgac
ccacctggtgtgactggtttgaataaaaaccgaagaagcctcccagtttc
catctgccggagctgtgagaccctggagggcccccagactgtggacactt
ggccccgatcccattccctggatgaccttcaagtggagcctggtgctgag
caagacgtgcctaccgaggtgacagaaccgccccctcagattgtacctga
agtgccacagaagacgaccgcctcttccacgaaggcccagcccctggagc
gagactctgctgtcgacaatgcattgctactgacccaaagcaagagattt
tctgaacctcagaaattgacaactaagaaactggagggctcaatcgcagc
ctctggtcgcggcctgtcaccccctcagtgtttgcccagaaactatgatg
ctcagcctcctggagctaaacacggtttagcaaggacgcctctggagggc
cacagaaaaggacacgagtttgaaggaacacaccatcccctgggcaccaa
agaaggggtagatgctgagcagagaatgcagcccaaaattccatcacagc
ctccacctgttcctgccaaaaagagcagagaacgccttgctaacggactc
caccctgttcccatgggccccagtggggccctccccagtcccgatgcgcc
atgcctgccagtgaaaaggggcagccccgccagccccaccagccctagcg
actgtcccccagcactggctcccaggcctctctcagggcaggcgcctggc
agcccaccaagcacaaggccgcccccctggctctcagagctccccgagaa
cacaagcctccaggagcacggtgtgaagctgggcccggctttgaccagga
aggtctcctgtgcccggggagtggatctagaaacgctcactgaaaacaag
ctgcacgctgaaggcatcgatctcacggaggagccgtattctgataagca
tggccgctgtgggattcctgaagccctggtgcagagatacgcagaggact
tggatcagcccgagcgggacgtcgccgccaacatggaccagatccgggtg
aagcagcttcggaagcagcaccgcatggcgattccaagtggtggactcac
ggaaatctgccgaaagcccgtctctcctgggtgcatttcgtctgtgtcag
attggctcatttccatcggtctgcccatgtacgccggcaccctctccacc
gcgggcttcagcacactgagccaagtgccttctctgtctcacacttgcct
tcaggaggccggcatcacagaggagagacacataagaaagctcctatctg
cagccagactcttcaaactgccgccaggccctgaggccatgtagcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTI
DAAAMEDAGAAGPGPEPEPEPEPEPEPAPEPEPEPKPGAGTSEAFSRLWT
DVMGILDGSLGNIDDLAQQYADYYNTCFSDVCERMEELRKRRVSQDLEVE
KPDASPTSLQLRSQIEESLGFCSAVSTPEVERKNPLHKSNSEDSSVGKGD
WKKKNKYFWQNFRKNQKGIMRQTSKGEDVGYVASEITMSDEERIQLMMMV
KEKMITIEEALARLMEYEAQHRQSAALDPADWPDGSYPTFDGSSNCNSRE
QSDDETEESVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTEGGEEHVFEN
SPVLDERSALYSGVHKKPLFFDGSPEKPPEDDSDSLTTSPSSSSLDTWGA
GRKLVKTFSKGESRGLIKPPKKMGTFFSYPEEEKAQKVSRSLTEGEMKKG
LGSLSHGRTCSFGGFDLTNRSLHVGSNNSDPMGKEGDFVYKEVIKSPTAS
RISLGKKVKSVKETMRKRMSKKYSSSVSEQDSGLDGMPGSPPPSQPDPEH
LDKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDD
EEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDIIDIISKPPMGTWMG
LLNNKVGTFKFIYVDVLSEDEEKPKRPTRRRRKGRPPQPKSVEDLLDRIN
LKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVELLQE
YDSNSDQSGSQEKLLVDSQGLSGCSPRDSGCYESSENLENGKTRKASLLS
AKSSTEPSLKSFSRNQLGNYPTLPLMKSGDALKQGQEEGRLGGGLAPDTS
KSCDPPGVTGLNKNRRSLPVSICRSCETLEGPQTVDTWPRSHSLDDLQVE
PGAEQDVPTEVTEPPPQIVPEVPQKTTASSTKAQPLERDSAVDNALLLTQ
SKRFSEPQKLTTKKLEGSIAASGRGLSPPQCLPRNYDAQPPGAKHGLART
PLEGHRKGHEFEGTHHPLGTKEGVDAEQRMQPKIPSQPPPVPAKKSRERL
ANGLHPVPMGPSGALPSPDAPCLPVKRGSPASPTSPSDCPPALAPRPLSG
QAPGSPPSTRPPPWLSELPENTSLQEHGVKLGPALTRKVSCARGVDLETL
TENKLHAEGIDLTEEPYSDKHGRCGIPEALVQRYAEDLDQPERDVAANMD
QIRVKQLRKQHRMAIPSGGLTEICRKPVSPGCISSVSDWLISIGLPMYAG
TLSTAGFSTLSQVPSLSHTCLQEAGITEERHIRKLLSAARLFKLPPGPEA
M*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16651
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_015278.3 CDS:; a492g (sil); a632t (K211M) a2651g (Q884R); Coding differences present in other published seqs (match AAH63279.1)