cDNA Clone RFX2

• Plasmid: pCMV5-HA RFX2
• Parent Plasmid: pCMV5 HA1
• Expressed: HA RFX2
• Genbank: NM_022841.5 GI:148613885
• Tag: HA
• Species: Human

Sequence of Insert

ggatccatggcagaggaacaacaacagccgccaccacagcagcctgatgc
ccatcagcagcttccccccagcgcccccaactcgggggtggccctgccag
cccttgtgcccgggctgccagggacagaggccagcgcgctgcaacacaag
atcaagaactccatctgcaaaactgtacaatctaaagtggactgcatttt
gcaagaagttgagaagtttacagacctagagaaactctacctctaccttc
agctgccttctggtctcagcaatggagagaaaagtgatcagaatgccatg
tcatctagtcgggcacaacaaatgcatgccttttcctggattcggaatac
cctagaggaacatccggagacttcactgcccaaacaggaagtctatgatg
agtacaagagctattgtgacaatcttggttaccatccattaagtgctgct
gattttggaaagatcatgaaaaacgtctttccaaacatgaaggcacgtcg
tttgggcacaagaggcaaatctaaatattgctacagtggactaagaaaaa
aagcttttgttcatatgccaacactgcccaaccttgactttcacaaaact
ggagatgggttggaaggagctgaaccttctgggcagcttcaaaatattga
tgaagaagttatctcttctgcttgccgtcttgtgtgtgagtgggcccaga
aagtgttaagccaaccatttgacaccgtcttggaattagcccgcttcctt
gtaaaaagtcactatataggcaccaagtcaatggcagctctaactgtaat
ggcagcagcaccagcaggaatgaaaggaattacccagccttctgctttta
tacctacagctgaaagtaattcctttcagcctcaggtgaagactttgcca
tctccaattgatgctaaacagcagttgcaacggaaaatccagaagaagca
gcaagaacagaaactacaatcccctttgccaggagaatctgcagcaaaaa
agtcagaaagtgctacaagcaatggagtgactaatcttcctaatggaaat
ccttcaatcctttctcctcaacctattggtatcgttgtggcagctgtccc
tagtcccattccggtccagcggactaggcaattggtaacttcaccgagtc
caatgagttcttctgacggcaaagttcttcccctcaatgtacaggtggtc
actcagcacatgcagtctgtgaaacaggcaccaaagactccccagaacgt
tccagccagtcctggtggggatcgttctgcccggcaccgttaccctcaga
tcttacccaaaccagcgaacaccagtgcactcaccattcgctctccaact
actgtcctctttactagtagtcccatcaaaactgctgttgtacccgcttc
acacatgagttctctaaatgtggtgaaaatgacaacaatatccctcacac
ccagcaacagtaacacccctcttaaacattctgcctcagtcagcagtgct
acaggaacaacagaagaatcaaggagtgttccacagatcaagaatggttc
tgtcgtgtcgcttcagtctcctgggtccaggagcagcagtgcggggggaa
catctgctgtggaagtcaaagtggaacccgaaacatcatcagatgagcat
cctgtacagtgccaagagaactctgatgaggctaaagctccccagacacc
tagtgcccttttggggcagaaaagtaatacagacggagcactgcagaaac
cttcaaatgaaggtgtcattgaaataaaagcaactaaggtctgtgaccag
aggaccaaatgtaaaagtcgctgtaatgaaatgctgccaggcacgtcaac
aggcaataatcaaagcactatcactctatcagttgcttctcagaacttaa
ctttcaccagcagcagctcaccacctaatggtgactcaatcaataaagac
cctaaattatgcactaaaagcccaagaaaacgactgtcttctacattgca
agagacccaggtgcctcctgtaaagaaaccaattgtggaacagctttcag
cagctaccatagaagggcagaaacaaggcagtgttaagaaggaccaaaag
gttccacattcagggaaaacagaaggttcaacagcaggtgctcagattcc
tagcaaggtatcagtaaatgtcagttcacacataggagcaaatcaaccct
tgaattcctctgcccttgttatcagtgattcagctttggaacagcaaaca
accccatcatcatctccagatataaaagtaaaacttgaaggaagtgtctt
tctcttggacagtgattcaaagtcagttggcagctttaatccaaatggat
ggcaacaaatcactaaagattctgagtttatatctgccagttgtgaacaa
cagcaagatatcagtgttatgacaattcctgagcactctgatatcaatga
cttagagaaatctgtttgggaattagaaggaatgccacaggacacatata
gccagcagctacatagccagatacaggaatcttctttaaatcaaatacaa
gcacattcttcagatcagttacctctgcaatctgaactgaaggagtttga
gccttctgtttcccagacaaatgaaagctactttccttttgatgatgaac
ttacacaagatagtattgtggaagagctggtgcttatggagcagcaaatg
tcaatgaacaattctcattcttacggcaactgtttgggaatgacccttca
gagtcagtcagtaactccaggagctccaatgtcatctcacacttccagca
cccacttctatcatccaatccacagcaatggcactccaatccacacaccc
acacccacacccacacccactcctactccaaccccaaccccaaccccgac
atctgaaatgattgctggatctcagagtctgtcacgggagagcccttgct
ccaggctagcccagactacacctgtggatagtgctttaggaagtagccga
catacacccattggtactccacattctaactgcagcagtagtgtcccccc
cagccctgttgaatgcaggaatccgtttgcattcactccaataagctcca
gtatggcatatcatgacgccagcattgtctcaagtagtcctgtgaaaccg
atgcaaagacccatggccacacaccctgacaaaaccaagcttgaatggat
gaataatgggtatagtggggttggtaattcatcagtttctggccatggta
ttctcccaagctatcaggaactagtggaagaccgtttcaggaaacctcat
gcttttgctgtgcctggacagtcttatcagtctcaatccagacatcatga
cactaattttggtcgtttgactcctgtctctcctgtgcagcatcaaggtg
ccactgtaaataacaccaacaaacaggagggttttgcagtccctgcccct
cttgataataaaggaactaattcatctgccagcagcaacttcagatgccg
gagtgtgagccctgctgttcatcgccaacgtaatcttagtggaagcaccc
tctatccagtatctaatatcccacgatctaatgtgaccccctttggaagt
ccagttaccccagaagttcatgttttcacaaatgttcacacagacgcatg
tgccaacaacatagctcaaagaagccaatcagttccattgacagtcatga
tgcagacagccttcccaaacgctcttcagaagcaagcaaacagtaaaaaa
ataaccaatgttttgttgagtaaacttgattccgacaatgatgatgcagt
gagaggtttgggaatgaacaacctgccctctaattatacagcccggatga
atctcactcagattttggaaccttccactgtttttcctagtgccaaccca
caaaatatgatcgattccagcacttctgtttatgagttccaaacaccatc
ttacctcaccaaaagtaatagcaccggtcagatcaatttttctcctggag
ataatcaagcacaatcagaaattggagagcaacaattagatttcaatagc
actgttaaagacctgttgagtggagacagcttgcaaaccaaccagcagct
ggtaggtcagggagcatctgatctcactaatactgcatctgatttctcta
gcgatatcaggttgtcttctgagctctcaggcagcatcaatgatttgaac
actttagacccaaatctactgtttgatccaggtcgtcagcagggacaaga
tgatgaagctacactggaagaattaaagaatgacccattatttcaacaaa
tttgcagtgaatccatgaattctatgacttcatcaggttttgaatggata
gaaagcaaggaccatcctactgttgaaatgttgggttaagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMAEEQQQPPPQQPDAHQQLPPSAPNSGVALPALVPGLP
GTEASALQHKIKNSICKTVQSKVDCILQEVEKFTDLEKLYLYLQLPSGLS
NGEKSDQNAMSSSRAQQMHAFSWIRNTLEEHPETSLPKQEVYDEYKSYCD
NLGYHPLSAADFGKIMKNVFPNMKARRLGTRGKSKYCYSGLRKKAFVHMP
TLPNLDFHKTGDGLEGAEPSGQLQNIDEEVISSACRLVCEWAQKVLSQPF
DTVLELARFLVKSHYIGTKSMAALTVMAAAPAGMKGITQPSAFIPTAESN
SFQPQVKTLPSPIDAKQQLQRKIQKKQQEQKLQSPLPGESAAKKSESATS
NGVTNLPNGNPSILSPQPIGIVVAAVPSPIPVQRTRQLVTSPSPMSSSDG
KVLPLNVQVVTQHMQSVKQAPKTPQNVPASPGGDRSARHRYPQILPKPAN
TSALTIRSPTTVLFTSSPIKTAVVPASHMSSLNVVKMTTISLTPSNSNTP
LKHSASVSSATGTTEESRSVPQIKNGSVVSLQSPGSRSSSAGGTSAVEVK
VEPETSSDEHPVQCQENSDEAKAPQTPSALLGQKSNTDGALQKPSNEGVI
EIKATKVCDQRTKCKSRCNEMLPGTSTGNNQSTITLSVASQNLTFTSSSS
PPNGDSINKDPKLCTKSPRKRLSSTLQETQVPPVKKPIVEQLSAATIEGQ
KQGSVKKDQKVPHSGKTEGSTAGAQIPSKVSVNVSSHIGANQPLNSSALV
ISDSALEQQTTPSSSPDIKVKLEGSVFLLDSDSKSVGSFNPNGWQQITKD
SEFISASCEQQQDISVMTIPEHSDINDLEKSVWELEGMPQDTYSQQLHSQ
IQESSLNQIQAHSSDQLPLQSELKEFEPSVSQTNESYFPFDDELTQDSIV
EELVLMEQQMSMNNSHSYGNCLGMTLQSQSVTPGAPMSSHTSSTHFYHPI
HSNGTPIHTPTPTPTPTPTPTPTPTPTSEMIAGSQSLSRESPCSRLAQTT
PVDSALGSSRHTPIGTPHSNCSSSVPPSPVECRNPFAFTPISSSMAYHDA
SIVSSSPVKPMQRPMATHPDKTKLEWMNNGYSGVGNSSVSGHGILPSYQE
LVEDRFRKPHAFAVPGQSYQSQSRHHDTNFGRLTPVSPVQHQGATVNNTN
KQEGFAVPAPLDNKGTNSSASSNFRCRSVSPAVHRQRNLSGSTLYPVSNI
PRSNVTPFGSPVTPEVHVFTNVHTDACANNIAQRSQSVPLTVMMQTAFPN
ALQKQANSKKITNVLLSKLDSDNDDAVRGLGMNNLPSNYTARMNLTQILE
PSTVFPSANPQNMIDSSTSVYEFQTPSYLTKSNSTGQINFSPGDNQAQSE
IGEQQLDFNSTVKDLLSGDSLQTNQQLVGQGASDLTNTASDFSSDIRLSS
ELSGSINDLNTLDPNLLFDPGRQQGQDDEATLEELKNDPLFQQICSESMN
SMTSSGFEWIESKDHPTVEMLG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16224
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee