cDNA Clone RBX1

• Plasmid: pFBDM-GST-Cullin4a, 6His-Ddb1; Rbx1
• Parent Plasmid: pFBDM
• Expressed: RBX1
• Genbank: NM_014248.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGCAGCGATGGATGTGGATACCCCGAGCGGCACCAACAGCGGCGCGGGCAAGAAGCGCTTTGAAGTGAAAAAGTGGAATGCAGTAGCCCTCTGGGCCTGGGATATTGTGGTTGATAACTGTGCCATCTGCAGGAACCACATTATGGATCTTTGCATAGAATGTCAAGCTAACCAGGCGTCCGCTACTTCAGAAGAGTGTACTGTCGCATGGGGAGTCTGTAACCATGCTTTTCACTTCCACTGCATCTCTCGCTGGCTCAAAACACGACAGGTGTGTCCATTGGACAACAGAGAGTGGGAATTCCAAAAGTATGGGCACTAGGCGGCCGC

Amino Acid Sequence

MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH*

• Vector Type: Baculovirus
• Antibiotic: Amp
• Full Snapgene Map: pFBDM-GST-Cullin4a, 6His-Ddb1; Rbx1113.87 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU21928
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Pme1/AvrII fragment containing Rbx1 from SC21707 was cloned into BstZ17I/Spe1 sites of SC21802. Prescence of all components confirmed by sequencing (BacF, pGEX F, MP7509/7510, p10F)
• Low Temp: No