cDNA Clone RALGAPA2 (mouse)

• Synonyms: A230067G21Rik, AS250, pp250
• Plasmid: pcDNA5-FRT/TO-GFP RALGAPA2 (mouse) L611-D1910(end)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-RALGAPA2 (mouse) L611-D1910(end)
• Genbank: NM_001033348.3 GI:158187504 with changes c4038g, a4620g, g5031a, c5034t, a5553g (all silent), a4645c (N1549H - present in other species).
• Tag: GFP
• Species: Mouse

Sequence of Insert

agatctctctgtgtgtacatttctcgggagctctgggatgactttctcagggtgctgtcatcccttacggaatgggaggaactcatcactgaatggtctaacatcatggattccttgacagccgtgcttgccagaactgtgtatggagttgaaatgacaaacctacctctagataagttaagtgaacaaaaggagaagaagcagcgtggcaaaggttgtatcctagaaccccaaaaaggaacagctgttgggagatccttttctctcagctggcgtagccatccagatgtgactgagccaatgagattcagaagtgccaccacatctggagcaccaggagtggagaaggcaagaaatactgtccgtcaaaaagcaactgaagtggaggagttccagcaggcagagagcaccgccgcagccgactgtgactatcttgtggtaggacagcagcaggtgccccggagcagcagcacctctgacatcactgagcgcttgtactcagactcctctcaaggttacagccccatgttgcctcatcctgctttttccacgacttctggggatcagagctctggtcctcatgcttgcacagctggccctttacctttgagccgtctccccagtgcaggtcaaaaggtagaacattcgcagaatttaagctcttcagaacccaagtctgttcaagagagtaaaggacatgtgactcatgaacatgaaggaataacaatgttggttcgaaggagcagcagtcccgctgagctggagctgaaggatgatttacagcaggcacatggaagatgcaggcagagacagacaagtgaaagtactggtagtgacacagttgtgggctacagcaatgaagcagagctacctgtgagcccgtggcaggcctgtgaggaggacccggacctgagcactcccacagatgctgtggctgactctgatgcccgccactggttacaactgagtcctactgatgcttcaaacttaacagacagcagagaatgccttgcagacgactgtagcattattgctggaggaaacctcactggttggcacccagactccgctgctgtgttatggcgaagagtcttgggaatccttggagatgtgaataacatccagtcgccgaagatccatgccaaagtttttggctatctctatgaactgtggtataaactggccaagatacgagataatttagcaataagcctggataaccagtcgtctccatctcctcctctcttgatcccaccactcagaatgtttgcatcatggctatttaaggctacaactctgccaaatgaatataaagaaggcaaactgcaagcctacaaactgatctgtgccatgatgaccagacgccaggatgttctgccaaactctgacttcctggtgcatttttaccttgtgatgcacctgggactaaccagcgaggaccaggatgtcttaaacaccatcataaaaaactgctcaccccgctttttctccctgggtttgcctggcttctctatgctggtgggggactttatcactgctgctgccagggtcctcagtacagacatgttggcggcaccccgttcagaagctctcactctccttggttcccttgtctgcttcccaaatacctaccaggagatccctctgctgcagtcagtgccagaagtgagtgatgttgtcacgggagcggaagacgtcaagcattacctcataaatattttactgaagaatgctaccgaggaaccaaatgaatgtgcaagatgcatcgccatttgctccctcggggtctggatctgtgaagagcttgcacagagtgccagccatcctcaggtgaaggatgccatcaatgtgataggtgtaactttgaagtttcctaacaagatcgtggctcaggtagcctgtgatgttcttcagttgttggtttcctactgggaaaagctgcagatgtttgaaactgctctaccccgcaaaatggcagaaattcttgtggccacaattgcgttccttttaccaagtgcagagtactcgtcagtggaaacagataaaaagtttattgtgtccttgctcctttgtctcttggactggtgcatggcgctgcccgtgagtgccctcctccaccctgtgtccacagcagttctagaggagctgcacccatcccgggcccccttgctggattatatctacagggttctgcactgctgtgtctgtggctcgagcacatacacacagcaaagtcactatactctgaccttggctgacctgtcatccacggattatgaccccttcctacctctggcaaacgtgaggaactccgagccaatccagtatcattcatcagcagacctggggaacctgctgaccgtggaagaggaaaagaagagaagaagtgtggaattgatccccctcactgcacgaatggtcatggcccacctagtgaaccacctaggccactatccactgagtgggggtcctgctgtgctgcacagcctggtcagtgagaaccatgacaatgcccatgtggaaggaactgagctgtcctcggaggtgttcaggagcccaaacctgcagctcttcgtatttaatgatagcaccctcatctcctatcttcagacaccagcagaaggaccagctggagggacttcagggggttccctctcagatgtgagagtcattgtgagggatatctcagggaagtactcttgggatggcaaggttttatatggacctctggaaggccgtttggcacccaatggaaggaacccctcatttcagatttctggctggcatcatcacacgtgtggacctcagaaagatttgtttcacggtgaggagggagatgatgtgcttgacaagttacttgaaaacattggccatacaagccccgaatgccttttaccatcacaactaaatctaaatgagccttccccaaccccatgtgcaatgaactgggaccaagagaaggcaatcatggaggtcatcctgcgccagagcgcacaggaagatgagtatgtgcagaggtgtaactcggattcttcagtaacggtcaccagccaagggcagccctcacccgtggagccccggggacccttttatttctgcaggttgttgcttgatgacttgggaatgaattcctgggacagaaggaagaattttcatttgttgaagaaaaattcaaaattattgagagagctaaagaatctggactcacgtcagtgccgcgagacccataagattgcagtgttttacattgctgaagggcaagaagacaagtgttccattctagccaatgaaagaggaagccaagcatatgaagactttgttgctggtcttggatgggaggtggatctctcaacccactgtgggtttatgggtggtcttcagcgcaatggcagcacgggacagacagccccttactatgctacctcgaccgtggaagtaattttccacgtttccactcggatgccatcagattcagatgattctctcaccaaaaagctccgtcacctggggaatgatgaggtccacatcgtctggtctgaacactccagggactaccgcaggggtattatcccaacagcctttggagacgtttccatcattatttacccaatgaagaatcatatgttctttatcacgataacgaagaagcctgaggttccattctttgggcccctgttcgatggagcgatcgtgagtgggaagctgctgccaagccttatctgtgccacgtgcatcaatgccagcagagcggtgaagtgcctcatcccactctatcagagcttctacgaagagcgagctctgtatctggaagcaataattcaaaaccaccgggaagtcatgacatttgaggatttcgctgctcaagtcttttctccctctcccagctactctgtcagtggaacggattaagcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDITERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKGHVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDPDLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSADLGNLLTVEEEKKRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHTCGPQKDLFHGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSVTVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSVSGTD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU40002
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All RalGAP alpha plasmids MUST be grown at 30 degrees C or less to prevent recombination.