cDNA Clone RALGAPA2 (mouse)

• Synonyms: A230067G21Rik, AS250, pp250
• Plasmid: pcDNA5-FRT/TO-GFP RALGAPA2 (mouse) M1-R617 (BglII/Not1)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-RALGAPA2 (mouse) M1-R617
• Genbank: NM_001033348.3 GI:158187504 with change a1307g (Q436R)
• Tag: GFP
• Species: Mouse

Sequence of Insert

agatctatgttctcccgaaggagccacggagatgtgaaaaagtccacgcagaaggtgctggaccccaagaaggacgtgctgacccgcctgaagcacctgcgggcgctgctggataacgtggatgcaagcgatcttaagcagttctttgagaccaactattctcagatatatttcatcttctatgaaaattttataacactggaaaatagtttgaagttaaaagggaataataagtcacaaagggaggagctggactccatcctctttctatttgaaaaaatactgcagtttctacctgaacgaattttctttcgatggcattaccagagtataggctcaactttaaagaagcttttacacactggaaattctattaagataagatgtgaaggaatcagactatttcttttgtggcttcaagcacttcagacaaactgtgcagaagagcaggtgctgattttcgcttgcctagtgcctggtttcccagcggtcttgtcatccaggggtccctgcacactggagacactcatcaaccctagccctagtatagttgatgcaaaaatatacccagaggaaatcaccccacttctgccagccatatcaggggagaagattgctgaggaccaaacctgcttttttcttcaaatactgttgaaatacatggttattcaggctgcaagcttggagtggaagaataaagagaatcaagatactggttttaaatttctttttacgttgtttcgaaagtattatcttcctcatttgtttccatcatttactaaattaaccaacatctacaaacctgtacttgaaattccccatttgagaccaaagccagtatatgttactgtaactcgagataatgaaacaatttacagtacaaaaattccatacatggcagctcgtgttgttttcattaaatggattgtaactttctttttggaaaaaaagtatctaactgcaacacaaaacactaaaaatggagttgatgtattgcctaaaatcatccagactgttggtggtggtgcaatacaagagaaggtaccagagctggatggtgctgggtccacagaacaggacaaaagccattctaacagcagcaccttgtctgaccgaagactcagcaactccagtctttgtagcattgaagaggagcatcggacagtgtatgagatggtgcagcggatcctcctgtcaacacggggttatgtgaattttgtgaatgaagtatttcgacaggcatttttgttgccttcctgtgagatatctgtaacaagaaaagtagttcaagtgtataggaaatggatcctccggaacaaacctgttttcatggaggaaccagataaaaaggatgttgctcaagaagatgctgacaaattaggactttcagagactgactccaaggaggcctcatctgaaagttctggtcataaacggtcatccagttggggacgcacgtattccttcacaagtgccatgagcagaggatgtgtgacagaggaggacaacacaaatgtgaaagctggggcccaagctatgttgcaggtctttcttacgaatgctgcaaatgtctttttgctggaaccatgtgccgaagttcccatgctcctgagagaacaagtagacgcctctaaagctgttctgattatttttcggcggatgataatggagcttacaatgaatcaaaagacatgggagcagatgttgcaaatccttctcaggataacagaagctgtcatgcagaagccaaaggataagcatgtcaaggacttgtttgcccagagcttggcagggttgctcttcaggacacttattgtggcttggatccgagcaaacctctgtgtgtacatttctcggtaagcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILRNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISR*

• Vector Type: Mammalian
• Antibiotic: Amp; Kan
• DU Number: DU40001
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All RalGAP alpha plasmids MUST be grown at 30 degrees C or less to prevent recombination.