Synonyms
A230067G21Rik, AS250, pp250
Plasmid
pcDNA5-FRT/TO-HA RALGAPA2 (mouse)
Parent Plasmid
pcDNA5 FRT/TO HA1
Expressed
HA-RALGAPA2 (mouse)
Genbank
NM_001033348.3 GI:with changes: a1307g (Q436R), c4038g, a4620g, g5031a, c5034t, a5553g (all silent), a4645c (N1549H - present in other species).
Tag
HA
Species
Mouse
Sequence of Insert
View Sequence
agatctatgttctcccgaaggagccacggagatgtgaaaaagtccacgcagaaggtgctggaccccaagaaggacgtgctgacccgcctgaagcacctgcgggcgctgctggataacgtggatgcaagcgatcttaagcagttctttgagaccaactattctcagatatatttcatcttctatgaaaattttataacactggaaaatagtttgaagttaaaagggaataataagtcacaaagggaggagctggactccatcctctttctatttgaaaaaatactgcagtttctacctgaacgaattttctttcgatggcattaccagagtataggctcaactttaaagaagcttttacacactggaaattctattaagataagatgtgaaggaatcagactatttcttttgtggcttcaagcacttcagacaaactgtgcagaagagcaggtgctgattttcgcttgcctagtgcctggtttcccagcggtcttgtcatccaggggtccctgcacactggagacactcatcaaccctagccctagtatagttgatgcaaaaatatacccagaggaaatcaccccacttctgccagccatatcaggggagaagattgctgaggaccaaacctgcttttttcttcaaatactgttgaaatacatggttattcaggctgcaagcttggagtggaagaataaagagaatcaagatactggttttaaatttctttttacgttgtttcgaaagtattatcttcctcatttgtttccatcatttactaaattaaccaacatctacaaacctgtacttgaaattccccatttgagaccaaagccagtatatgttactgtaactcgagataatgaaacaatttacagtacaaaaattccatacatggcagctcgtgttgttttcattaaatggattgtaactttctttttggaaaaaaagtatctaactgcaacacaaaacactaaaaatggagttgatgtattgcctaaaatcatccagactgttggtggtggtgcaatacaagagaaggtaccagagctggatggtgctgggtccacagaacaggacaaaagccattctaacagcagcaccttgtctgaccgaagactcagcaactccagtctttgtagcattgaagaggagcatcggacagtgtatgagatggtgcagcggatcctcctgtcaacacggggttatgtgaattttgtgaatgaagtatttcgacaggcatttttgttgccttcctgtgagatatctgtaacaagaaaagtagttcaagtgtataggaaatggatcctccggaacaaacctgttttcatggaggaaccagataaaaaggatgttgctcaagaagatgctgacaaattaggactttcagagactgactccaaggaggcctcatctgaaagttctggtcataaacggtcatccagttggggacgcacgtattccttcacaagtgccatgagcagaggatgtgtgacagaggaggacaacacaaatgtgaaagctggggcccaagctatgttgcaggtctttcttacgaatgctgcaaatgtctttttgctggaaccatgtgccgaagttcccatgctcctgagagaacaagtagacgcctctaaagctgttctgattatttttcggcggatgataatggagcttacaatgaatcaaaagacatgggagcagatgttgcaaatccttctcaggataacagaagctgtcatgcagaagccaaaggataagcatgtcaaggacttgtttgcccagagcttggcagggttgctcttcaggacacttattgtggcttggatccgagcaaacctctgtgtgtacatttctcgggagctctgggatgactttctcagggtgctgtcatcccttacggaatgggaggaactcatcactgaatggtctaacatcatggattccttgacagccgtgcttgccagaactgtgtatggagttgaaatgacaaacctacctctagataagttaagtgaacaaaaggagaagaagcagcgtggcaaaggttgtatcctagaaccccaaaaaggaacagctgttgggagatccttttctctcagctggcgtagccatccagatgtgactgagccaatgagattcagaagtgccaccacatctggagcaccaggagtggagaaggcaagaaatactgtccgtcaaaaagcaactgaagtggaggagttccagcaggcagagagcaccgccgcagccgactgtgactatcttgtggtaggacagcagcaggtgccccggagcagcagcacctctgacatcactgagcgcttgtactcagactcctctcaaggttacagccccatgttgcctcatcctgctttttccacgacttctggggatcagagctctggtcctcatgcttgcacagctggccctttacctttgagccgtctccccagtgcaggtcaaaaggtagaacattcgcagaatttaagctcttcagaacccaagtctgttcaagagagtaaaggacatgtgactcatgaacatgaaggaataacaatgttggttcgaaggagcagcagtcccgctgagctggagctgaaggatgatttacagcaggcacatggaagatgcaggcagagacagacaagtgaaagtactggtagtgacacagttgtgggctacagcaatgaagcagagctacctgtgagcccgtggcaggcctgtgaggaggacccggacctgagcactcccacagatgctgtggctgactctgatgcccgccactggttacaactgagtcctactgatgcttcaaacttaacagacagcagagaatgccttgcagacgactgtagcattattgctggaggaaacctcactggttggcacccagactccgctgctgtgttatggcgaagagtcttgggaatccttggagatgtgaataacatccagtcgccgaagatccatgccaaagtttttggctatctctatgaactgtggtataaactggccaagatacgagataatttagcaataagcctggataaccagtcgtctccatctcctcctctcttgatcccaccactcagaatgtttgcatcatggctatttaaggctacaactctgccaaatgaatataaagaaggcaaactgcaagcctacaaactgatctgtgccatgatgaccagacgccaggatgttctgccaaactctgacttcctggtgcatttttaccttgtgatgcacctgggactaaccagcgaggaccaggatgtcttaaacaccatcataaaaaactgctcaccccgctttttctccctgggtttgcctggcttctctatgctggtgggggactttatcactgctgctgccagggtcctcagtacagacatgttggcggcaccccgttcagaagctctcactctccttggttcccttgtctgcttcccaaatacctaccaggagatccctctgctgcagtcagtgccagaagtgagtgatgttgtcacgggagcggaagacgtcaagcattacctcataaatattttactgaagaatgctaccgaggaaccaaatgaatgtgcaagatgcatcgccatttgctccctcggggtctggatctgtgaagagcttgcacagagtgccagccatcctcaggtgaaggatgccatcaatgtgataggtgtaactttgaagtttcctaacaagatcgtggctcaggtagcctgtgatgttcttcagttgttggtttcctactgggaaaagctgcagatgtttgaaactgctctaccccgcaaaatggcagaaattcttgtggccacaattgcgttccttttaccaagtgcagagtactcgtcagtggaaacagataaaaagtttattgtgtccttgctcctttgtctcttggactggtgcatggcgctgcccgtgagtgccctcctccaccctgtgtccacagcagttctagaggagctgcacccatcccgggcccccttgctggattatatctacagggttctgcactgctgtgtctgtggctcgagcacatacacacagcaaagtcactatactctgaccttggctgacctgtcatccacggattatgaccccttcctacctctggcaaacgtgaggaactccgagccaatccagtatcattcatcagcagacctggggaacctgctgaccgtggaagaggaaaagaagagaagaagtgtggaattgatccccctcactgcacgaatggtcatggcccacctagtgaaccacctaggccactatccactgagtgggggtcctgctgtgctgcacagcctggtcagtgagaaccatgacaatgcccatgtggaaggaactgagctgtcctcggaggtgttcaggagcccaaacctgcagctcttcgtatttaatgatagcaccctcatctcctatcttcagacaccagcagaaggaccagctggagggacttcagggggttccctctcagatgtgagagtcattgtgagggatatctcagggaagtactcttgggatggcaaggttttatatggacctctggaaggccgtttggcacccaatggaaggaacccctcatttcagatttctggctggcatcatcacacgtgtggacctcagaaagatttgtttcacggtgaggagggagatgatgtgcttgacaagttacttgaaaacattggccatacaagccccgaatgccttttaccatcacaactaaatctaaatgagccttccccaaccccatgtgcaatgaactgggaccaagagaaggcaatcatggaggtcatcctgcgccagagcgcacaggaagatgagtatgtgcagaggtgtaactcggattcttcagtaacggtcaccagccaagggcagccctcacccgtggagccccggggacccttttatttctgcaggttgttgcttgatgacttgggaatgaattcctgggacagaaggaagaattttcatttgttgaagaaaaattcaaaattattgagagagctaaagaatctggactcacgtcagtgccgcgagacccataagattgcagtgttttacattgctgaagggcaagaagacaagtgttccattctagccaatgaaagaggaagccaagcatatgaagactttgttgctggtcttggatgggaggtggatctctcaacccactgtgggtttatgggtggtcttcagcgcaatggcagcacgggacagacagccccttactatgctacctcgaccgtggaagtaattttccacgtttccactcggatgccatcagattcagatgattctctcaccaaaaagctccgtcacctggggaatgatgaggtccacatcgtctggtctgaacactccagggactaccgcaggggtattatcccaacagcctttggagacgtttccatcattatttacccaatgaagaatcatatgttctttatcacgataacgaagaagcctgaggttccattctttgggcccctgttcgatggagcgatcgtgagtgggaagctgctgccaagccttatctgtgccacgtgcatcaatgccagcagagcggtgaagtgcctcatcccactctatcagagcttctacgaagagcgagctctgtatctggaagcaataattcaaaaccaccgggaagtcatgacatttgaggatttcgctgctcaagtcttttctccctctcccagctactctgtcagtggaacggattaagcggccgc
Amino Acid Sequence
View Sequence
MYPYDVPDYAGSMFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKYMVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRPKPVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGSTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRTVYEMVQRILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILRNKPVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCILEPQKGTAVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNTVRQKATEVEEFQQAESTAAADCDYLVVGQQQVPRSSSTSDITERLYSDSSQGYSPMLPHPAFSTTSGDQSSGPHACTAGPLPLSRLPSAGQKVEHSQNLSSSEPKSVQESKGHVTHEHEGITMLVRRSSSPAELELKDDLQQAHGRCRQRQTSESTGSDTVVGYSNEAELPVSPWQACEEDPDLSTPTDAVADSDARHWLQLSPTDASNLTDSRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDMLAAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTAVLEELHPSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSSTDYDPFLPLANVRNSEPIQYHSSADLGNLLTVEEEKKRRSVELIPLTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTSGGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNGRNPSFQISGWHHHTCGPQKDLFHGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSVTVTSQGQPSPVEPRGPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSPSYSVSGTD*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU40006
Unit Source
Location
PPU
MTA
Notes
Very poor growth in E. coli. Grow at 30 degrees over 2 nights.
Price
£125.00
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