cDNA Clone RALGAPA1-L (mouse)

• Synonyms: 2310003F20Rik, 4930400K19Rik, I563624, Garnl1, GRIPE, mKIAA0884, Tulip
• Plasmid: pcDNA5-FRT/TO-HA RALGAPA1-L (mouse) M1-A1104
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA-RALGAPA1-L (mouse) M1-A1104
• Genbank: NM_019994.4 GI:51230428 Sequence differences compared with NM_019994.4 CDS: insertion of 47 residues after V754 a3400g (T1134A) (Agreed with Shuai Chen to leave in and call this version RalGAPa1-L 08/08/11)
• Tag: HA
• Species: Mouse

Sequence of Insert

agatctatgttctctaagaagccgcacggggacgtgaagaagtccacccagaaggtgctggacaccaagaaggacgcgctgacccgcctcaagcacctgcgcatcgtcatcgagaatgcagaatctattgaccttaaacagttttttgaccaacatttttcacatatatactatgtgttttttgaaaattttgtgactattgaagctagtcttaaacagaaaggtcacaagtctcaaagagaggaattagatgctatactttttatttttgagaaaattttacaacttcttccagaaagaattcatcagcgatggcaattccatagtattgggttgattttaaagaaactactgcacacaggaaactccttaaagatcagacgggaaggtgttcgtctcttcttgttatggttgcaagctcttcaggataactgcagcaaagaacagctctggatgttttcatgcttgatccctggattctcagcaccacaatccgaatatggacctcggactttagataatcttattaatcctccactcagccttcaagaaactcaagtaactatagaggaagtcactcctcttgttccgccacagtcaggagataaaggacaagaagacctcacaagctattttcttgaagcacttttaaaatacatagtaattcaggtaaaaagtttagaatggaagaacaaagaaaaccaagaaaggggattttcatttttgttttcacattttaagaagttttacttgccttatattttcccaaacatttgcaaggagaatagtttgtatcatcctgtacttgatatcccacagatcagaccaaagcctcattatgtgatgataaagaaggatgctgaaaccaatgaaacaatatattgtacaaaagagcctttcatccaggctcgggttattgtcattcgttggctggtttctttctggctggagccaaaaccacattcaggacctaatattcctgggatggaaggtgaagtcttgccaaagaatattcagagagcagctgctagtttggtttccagagaagaaagcaaaaatgatactgttgataaagtagacaagtctgcagagcctgagcagtctcattccaacacaagcactctcacggagcgagagcctagctcttccagtctctgcagcatcgacgaagaacatctcacagacatcgaaattgttcgcagagtgttttcatctaagagaagcaatgtaaactttgtcacagagatatttcgtcaggcatttttattaccaatttgtgaagcagcagctatgagaaaagtggtgaaagtgtatcaagaatggattcaacaagaggaaaagcctttgtttatgcaagagcctgaggacactgcgatcacttgttcagatataccctgcagcgaaactgttgcagaccatgacagtgcaatagaagatggagagaaaagagaagaggaaaatgggaccagtacttctgaacatgttcgcaattctagctggacaaaaaatggttcctaccaagaagcttttcatgtctgtgaagaagccacagaacagaatattcaagctggcactcaggccgttttgcaggtgtttattataaactcatcgaacatatttcttcttgaacctgcaaatgaaataaagaatcttctggatgagcatacagatatgtgtaaacgcattcttaatatttatcgatacatggttgtgcaagtatcaatggacaaaaagacctgggaacagatgctgctcgtgctgctcagagtcacggagtccgtactgaagatgtcgtcacaagcttttctgcagttccaggggaaaaaaagcatgaccctggcgggtcgacttgcaggaccactttttcagactctcattgttgcctggatcaaagcaaatctaaatgtatacatttctcgagaactttgggatgacttactttcggtattgtcatcattgacctattgggaagaattggccactgagtggtcactgaccatggagacactgactaaagttttggctagaaatttatatagtttggatctcagtgatttgccattggataagctcagtgaacagaaacaaaaaaagcacaaagggaaaggagttggacacgaatttcagaaagtttcagttgacaagtcattttctagaggatggagtcgcgatcagcctggccaggcacctatgagacagagaagtgcaacgaccactggctccccaggaactgagaaggccaggagtatagtacggcaaaaaactgttgCCATGAGAAGCCGATCCATTGGTGAATGTGCTCTGCCATCGGCCTATATTCGCAGTGCTAAAAGTGCTCCTGTTCTGATCCATACTTCCAAACCCTTCTTGCCTGATATTGTTCTCACTCCCCTTTCTGATGAGCTTTCAGatattgatgatgctcaaatccttccccgctcaaccagagtcagacatttttcacagagtgaagacactggaaatgaagtttttggtgctttgcatgaggaacagccactgcctcgaagtagcagcacttctgacatcttggaaccattcactgttgagcgagccaaagtaaataaagaggacacgagccccaaactgcctcctcttaatagtgagactggtggcaacagcgccaatgttcctgacctgatggatgagtttatcgcagagcgacttcgaagtggcaatgcctcgactatgacaagaagggggagtagtccaggcagccttgaaatcccgaaagacctccctgatattctaaacaagcagaaccagatgcggcctgttgatgaccctggtgtaccctccgaatggacgtctccagccagtgcagggagcagtgatctcatgagctcagatagtcactcggattccttcagtgccttccagtgtgagggacgaaaatttgacaactttggcttcgggactgacattgggatcccatcttctgctgatgtggatttaggttctggccatcatcaaagcactgaggagcaggaagtggccagcctaactacgcttcatttagattcagaaacaagcagccttaatcagcaagccttctctgcagaagttgcaaccgttactggttcagaaagtgcttcccccgttcattcagctctgggatccaggtcgcagaccccctccccatctacactgagcagagctcacatagaacagaaggacctacagctcgatgagaagctccaccactctgttcttcagacgccggatgacctagaaatcagtgaatttccatctgaatgttgtagcgtgatggcaggaggtactctcactgggtggcatgcttaagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLSLQETQVTIEEVTPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQIRPKPHYVMIKKDAETNETIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESKNDTVDKVDKSAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEDTAITCSDIPCSETVADHDSAIEDGEKREEENGTSTSEHVRNSSWTKNGSYQEAFHVCEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKSMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRSTRVRHFSQSEDTGNEVFGALHEEQPLPRSSSTSDILEPFTVERAKVNKEDTSPKLPPLNSETGGNSANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCEGRKFDNFGFGTDIGIPSSADVDLGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPSPSTLSRAHIEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36984
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All RalGAP alpha plasmids MUST be grown at 30 degrees C or less to prevent recombination.