cDNA Clone Triad1

• Synonyms: ARIH2, ARI2, FLJ10938, FLJ33921
• Plasmid: pcDNA5-FRT/TO-GFP TRIAD1 (V141R/H158A)
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: GFP TRIAD1 (V141R/H158A)
• Genbank: NP_006312
• Tag: GFP
• Species: Human

Sequence of Insert

ATGTCAGTGGACATGAATAGCCAGGGGTCTGACAGCAATGAAGAGGACTATGACCCAAATTGTGAGGAAGAGGAAGAAGAAGAAGAAGACGACCCTGGGGACATAGAGGACTATTACGTGGGAGTAGCCAGCGATGTGGAGCAGCAGGGGGCTGATGCCTTTGATCCCGAGGAGTACCAGTTCACTTGCTTGACCTACAAGGAATCTGAGGGTGCCCTCAATGAGCACATGACCAGCTTAGCTTCTGTCCTAAAGGTATCTCATTCAGTTGCTAAACTTATATTAGTTAATTTCCACTGGCAAGTTTCAGAGATATTGGACAGATACAAGTCCAATTCTGCTCAACTGCTTGTTGAGGCTCGAGTTCAGCCTAATCCATCAAAACATGTTCCCACATCCCATCCCCCTCACCACTGTGCAcgGTGTATGCAGTTTGTGCGAAAGGAAAACCTACTCTCTCTGGCCTGTCAGgcCCAGTTTTGCCGCAGCTGCTGGGAGCAGCACTGCTCAGTTCTCGTCAAGGACGGCGTGGGCGTGGGAGTCTCTTGCATGGCTCAGGACTGTCCACTCCGTACACCAGAGGACTTTGTGTTTCCATTGCTTCCCAATGAAGAATTGAGAGAGAAATACAGGCGCTACCTCTTCAGGGACTATGTGGAGAGTCATTACCAGCTCCAGCTGTGCCCTGGTGCAGACTGCCCCATGGTTATTCGGGTACAGGAGCCTAGAGCTCGCCGAGTACAGTGCAATCGGTGCAACGAGGTCTTCTGTTTCAAGTGTCGTCAGATGTATCACGCACCCACAGACTGTGCCACAATCCGGAAATGGCTCACGAAGTGTGCAGACGACTCTGAAACAGCCAACTACATTAGTGCTCACACTAAAGACTGTCCCAAGTGCAACATCTGCATTGAGAAGAATGGAGGCTGCAATCACATGCAATGCTCCAAATGTAAACACGACTTCTGCTGGATGTGTCTAGGAGATTGGAAGACTCATGGCAGTGAATACTATGAGTGCAGTCGTTACAAGGAGAATCCTGACATCGTGAACCAGAGCCAACAAGCCCAGGCGAGGGAAGCCCTCAAGAAGTACTTATTCTACTTTGAGAGGTGGGAAAACCACAATAAAAGCTTGCAGCTAGAGGCACAGACATACCAGCGGATTCACGAGAAGATTCAGGAGAGGGTCATGAACAATCTGGGGACATGGATCGACTGGCAGTACCTACAGAATGCTGCCAAGCTCTTGGCCAAGTGTCGATACACCCTGCAATACACCTACCCATATGCATATTACATGGAGTCCGGACCCAGGAAGAAGCTGTTTGAATACCAGCAGGCTCAGCTGGAGGCTGAGATCGAAAACCTCTCATGGAAAGTGGAGCGTGCAGACAGCTATGACAGAGGGGACTTGGAGAACCAGATGCATATAGCGGAGCAGCGGAGGAGAACCCTGCTGAAAGATTTCCATGACACCTAA

Amino Acid Sequence

MSVDMNSQGSDSNEEDYDPNCEEEEEEEEDDPGDIEDYYVGVASDVEQQGADAFDPEEYQFTCLTYKESEGALNEHMTSLASVLKVSHSVAKLILVNFHWQVSEILDRYKSNSAQLLVEARVQPNPSKHVPTSHPPHHCARCMQFVRKENLLSLACQAQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU22136
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: V141R mutation to be introduced into the existing pcDNA5-FRT/TO-GFP-Triad1 (H158A) construct in order to create a double mutant