cDNA Clone VPS15

• Plasmid: pCMV5-HA3 VPS15 (has R1221G, derived from MP‚Äôs VPS15 clones)
• Parent Plasmid: pCMV5 HA3
• Expressed: HA-VPS15 (has R1221G, derived from MP‚Äôs VPS15 clones)
• Genbank: NM_014602
• Tag: HA
• Species: Human

Sequence of Insert

gtcgacatgggaaatcagcttgctggcattgctccctcccagatcctttctgtagagagttatttttcagatattcatgactttgaatatgataaaagcctggggagtactcggttttttaaagttgctcgagccaagcaccgagaaggcctggtcgttgtgaaggtttttgcaattcaggatcccacattgcctttaaccagctataaacaagagctggaggaactgaaaatcaggcttaattctgcacagaattgtctacctttccagaaagcatcagaaaaagcatctgagaaagcagctatg
ctctttaggcagtatgtgcgagacaatctctatgatcgcatcagtacccg
tccattcttgaataacattgagaagcgctggattgccttccagatcctga
cagctgtggaccaagcacacaaatctggagttcgtcatggggacatcaag
actgagaatgtgatggtcaccagttggaattgggttcttctaactgattt
tgccagttttaagcccacttatcttccagaagacaacccggcagatttca
attatttctttgacacatcacggaggagaacttgctatattgctcctgaa
cgttttgttgatggtgggatgtttgccactgagttagaatatatgagaga
tccttcaactccgcttgtagacttaaatagcaatcagagaacaagaggag
agttgaagagagcaatggacatcttttcagcaggttgtgtgatagctgag
ctttttacagaaggtgtaccattatttgatctctctcaacttttggctta
tagaaatggacattttttccctgaacaagtgctaaataaaattgaagatc
acagtatcagagaattggtaactcagatgattcaccgtgagccagataaa
cgtttagaggcagaagattacttaaaacagcagcgtggcaatgcctttcc
tgaaatattttacacttttcttcagccctacatggcccagtttgccaagg
aaacgtttctttctgcagatgagcgtattctggttatacggaaggatttg
ggcaacattattcacaatctctgtggacatgatctgccagaaaaagccga
aggagagcctaaggaaaatgggctggttatcttggtatctgttataacat
cctgcctacagacccttaaatactgtgattccaaactagctgctttggaa
ctgattcttcatttggctccaagattaagtgttgaaatccttttggatcg
tattactccatatcttttgcatttcagcaatgactctgttcctagggtga
gggctgaagccttgaggacgttgaccaaagttcttgctctcgtcaaagag
gttcctcgtaatgatatcaatatttatccggaatacattctgccaggcat
agcccacttagcccaagatgatgctactatcgttagactagcctatgctg
aaaacatagctctgctggcagaaacagctctgagattcctggaattagta
cagttaaaaaatcttaatatggaaaatgaccccaataatgaagaaataga
tgaggttacacatccaaatggaaattatgacacagagctccaagccttac
atgaaatggtccagcagaaagttgttactttgctaagtgaccctgaaaat
attgtaaaacaaaccttgatggaaaatggaataacacggctgtgtgtatt
ctttggacgtcagaaagccaacgatgttttgttgtcccacatgattactt
tcctaaatgataagaatgattggcatctacgtggagcattttttgatagt
atagttggtgttgctgcctatgttggctggcaaagctcctcaattctcaa
gcctctgctgcaacaaggtcttagtgatgctgaggaatttgtcattgtga
aagctctttatgcccttacttgtatgtgccagttaggactgctacaaaaa
ccccatgtttacgaatttgccagtgatattgcccccttcctgtgtcatcc
caatttatggatacgttatggtgccgtgggatttatcacagtggtagctc
gtcaaataagtacagctgatgtctactgtaaactgatgccttatcttgac
ccatatattacccaaccaataatacagattgaaagaaaacttgttctgct
cagtgttttaaaggaaccagtaagtcgttctatatttgattatgctttga
ggtctaaagatattactagcttgttcagacatcttcacatgcgtcagaag
aaacgaaatggttctcttcccgactgccctccgccagaggatcctgccat
agcacagcttctgaagaagttgctctcacagggaatgacagaggaagagg
aagacaaacttctggcactgaaagacttcatgatgaaatctaataaagca
aaggccaatatagtggaccagagccatcttcatgatagtagtcagaaagg
tgtaattgacttggcagctttaggcataactgggagacaagttgatcttg
ttaaaaccaaacaagaaccagatgacaaacgggccagaaaacatgtaaaa
caagactcaaatgtaaatgaagaatggaaaagcatgtttgggtcactgga
cccaccaaacatgccacaggccctacctaaagggagtgatcaggaggtga
ttcagactgggaaacctcctcgttccgagtcctctgctggcatttgtgtc
cctttgtcaacttcttcacaggttccagaagtgacaactgtccaaaataa
aaaaccagtaataccggttttaagtagtacaatcttaccatccacctatc
agattcgaattacaacttgtaaaactgaacttcagcaactcatccagcaa
aagcgggagcagtgcaatgctgagagaatagctaagcagatgatggaaaa
tgctgaatgggagagtaaaccaccaccacctggatggcgtcctaaagggc
tgttagttgcccatcttcatgagcataaatctgctgtgaatcgaattaga
gtctctgatgaacactcactttttgcaacatgttcaaatgatggcacagt
gaaaatctggaacagtcaaaagatggaggggaagaccaccactaccagat
ctattcttacatacagccgaattggaggacgagtcaagacgctcacattc
tgccaaggctcccactatttagccatagcatctgataatggtgctgtcca
gcttcttggaattgaggcttctaagctgcccaagtctcctaaaatccatc
ctctacaaagcagaattctagatcagaaggaggacggttgtgttgtggat
atgcatcacttcaactctggagcacagtctgttcttgcctatgccactgt
gaatggctctctggttggctgggaccttaggtcttcaagcaatgcgtgga
ctttaaagcatgatttaaagtcgggcctcatcacttcctttgctgtggac
atccaccaatgctggctctgcattggtacaagcagtggtaccatggcttg
ttgggacatgaggttccagttgccaatttcaagtcactgtcatccttcca
gggctcgaatcagacgcctctcgatgcaccctctgtatcagtcctgggtg
attgcagctgttcagggcaacaacgaagtgtccatgtgggacatggagac
tggtgacagaggatttactctctgggccagcagtgcaccaccactttctg
aattacagccttctcctcatagcgtccatggtatctactgtagtcctgca
gatggaaatcctatcctactaacagctggctcagatatgaaaataaggtt
ttgggacttggcttacccagaaaggtcctatgttgttgcaggaagtacta
gttccccatctgtgtcctactacaggaaaataattgaaggcactgaagtt
gtccaggaaattcagaataagcagaaagtaggaccaagtgatgacacccc
tcgaaggggcccagagtccctgcccgtgggacatcatgacatcatcactg
atgtcgccacattccagaccacacagggcttcatcgtaactgcttctaga
gatgggattgtgaaggtgtggaaataagcggccgc

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVDMGNQLAGIAPSQILSVESYFSDIHDFEYDKS
LGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQELEELKIRLNSA
QNCLPFQKASEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQ
ILTAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPA
DFNYFFDTSRRRTCYIAPERFVDGGMFATELEYMRDPSTPLVDLNSNQRT
RGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNKI
EDHSIRELVTQMIHREPDKRLEAEDYLKQQRGNAFPEIFYTFLQPYMAQF
AKETFLSADERILVIRKDLGNIIHNLCGHDLPEKAEGEPKENGLVILVSV
ITSCLQTLKYCDSKLAALELILHLAPRLSVEILLDRITPYLLHFSNDSVP
RVRAEALRTLTKVLALVKEVPRNDINIYPEYILPGIAHLAQDDATIVRLA
YAENIALLAETALRFLELVQLKNLNMENDPNNEEIDEVTHPNGNYDTELQ
ALHEMVQQKVVTLLSDPENIVKQTLMENGITRLCVFFGRQKANDVLLSHM
ITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDAEEFV
IVKALYALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVGFITV
VARQISTADVYCKLMPYLDPYITQPIIQIERKLVLLSVLKEPVSRSIFDY
ALRSKDITSLFRHLHMRQKKRNGSLPDCPPPEDPAIAQLLKKLLSQGMTE
EEEDKLLALKDFMMKSNKAKANIVDQSHLHDSSQKGVIDLAALGITGRQV
DLVKTKQEPDDKRARKHVKQDSNVNEEWKSMFGSLDPPNMPQALPKGSDQ
EVIQTGKPPRSESSAGICVPLSTSSQVPEVTTVQNKKPVIPVLSSTILPS
TYQIRITTCKTELQQLIQQKREQCNAERIAKQMMENAEWESKPPPPGWRP
KGLLVAHLHEHKSAVNRIRVSDEHSLFATCSNDGTVKIWNSQKMEGKTTT
TRSILTYSRIGGRVKTLTFCQGSHYLAIASDNGAVQLLGIEASKLPKSPK
IHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGSLVGWDLRSSSN
AWTLKHDLKSGLITSFAVDIHQCWLCIGTSSGTMACWDMRFQLPISSHCH
PSRARIRRLSMHPLYQSWVIAAVQGNNEVSMWDMETGDRGFTLWASSAPP
LSELQPSPHSVHGIYCSPADGNPILLTAGSDMKIRFWDLAYPERSYVVAG
STSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDI
ITDVATFQTTQGFIVTASRDGIVKVWK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11096
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Differences compared with NM_014602 CDS:; t387c (silent), a3661g (R1221G*), a3573g (silent); *present in MP's VPS15 clones.; ; Estimated MW of expressed protein : 155211 Daltons