cDNA Clone RALGAPA1-L (mouse)

• Plasmid: pSC-B-RALGAPA1-L nt1-3313 (Sph1)
• Parent Plasmid: pSC-B
• Genbank: insertion of 47 residues after V754 a3400g (T1134A) (Agreed with Shuai Chen to leave in and call this version RalGAPa1-L 08/08/11)
• Species: Mouse

Sequence of Insert

agatctatgttctctaagaagccgcacggggacgtgaagaagtccacccagaaggtgctggacaccaagaaggacgcgctgacccgcctcaagcacctgcgcatcgtcatcgagaatgcagaatctattgaccttaaacagttttttgaccaacatttttcacatatatactatgtgttttttgaaaattttgtgactattgaagctagtcttaaacagaaaggtcacaagtctcaaagagaggaattagatgctatactttttatttttgagaaaattttacaacttcttccagaaagaattcatcagcgatggcaattccatagtattgggttgattttaaagaaactactgcacacaggaaactccttaaagatcagacgggaaggtgttcgtctcttcttgttatggttgcaagctcttcaggataactgcagcaaagaacagctctggatgttttcatgcttgatccctggattctcagcaccacaatccgaatatggacctcggactttagataatcttattaatcctccactcagccttcaagaaactcaagtaactatagaggaagtcactcctcttgttccgccacagtcaggagataaaggacaagaagacctcacaagctattttcttgaagcacttttaaaatacatagtaattcaggtaaaaagtttagaatggaagaacaaagaaaaccaagaaaggggattttcatttttgttttcacattttaagaagttttacttgccttatattttcccaaacatttgcaaggagaatagtttgtatcatcctgtacttgatatcccacagatcagaccaaagcctcattatgtgatgataaagaaggatgctgaaaccaatgaaacaatatattgtacaaaagagcctttcatccaggctcgggttattgtcattcgttggctggtttctttctggctggagccaaaaccacattcaggacctaatattcctgggatggaaggtgaagtcttgccaaagaatattcagagagcagctgctagtttggtttccagagaagaaagcaaaaatgatactgttgataaagtagacaagtctgcagagcctgagcagtctcattccaacacaagcactctcacggagcgagagcctagctcttccagtctctgcagcatcgacgaagaacatctcacagacatcgaaattgttcgcagagtgttttcatctaagagaagcaatgtaaactttgtcacagagatatttcgtcaggcatttttattaccaatttgtgaagcagcagctatgagaaaagtggtgaaagtgtatcaagaatggattcaacaagaggaaaagcctttgtttatgcaagagcctgaggacactgcgatcacttgttcagatataccctgcagcgaaactgttgcagaccatgacagtgcaatagaagatggagagaaaagagaagaggaaaatgggaccagtacttctgaacatgttcgcaattctagctggacaaaaaatggttcctaccaagaagcttttcatgtctgtgaagaagccacagaacagaatattcaagctggcactcaggccgttttgcaggtgtttattataaactcatcgaacatatttcttcttgaacctgcaaatgaaataaagaatcttctggatgagcatacagatatgtgtaaacgcattcttaatatttatcgatacatggttgtgcaagtatcaatggacaaaaagacctgggaacagatgctgctcgtgctgctcagagtcacggagtccgtactgaagatgtcgtcacaagcttttctgcagttccaggggaaaaaaagcatgaccctggcgggtcgacttgcaggaccactttttcagactctcattgttgcctggatcaaagcaaatctaaatgtatacatttctcgagaactttgggatgacttactttcggtattgtcatcattgacctattgggaagaattggccactgagtggtcactgaccatggagacactgactaaagttttggctagaaatttatatagtttggatctcagtgatttgccattggataagctcagtgaacagaaacaaaaaaagcacaaagggaaaggagttggacacgaatttcagaaagtttcagttgacaagtcattttctagaggatggagtcgcgatcagcctggccaggcacctatgagacagagaagtgcaacgaccactggctccccaggaactgagaaggccaggagtatagtacggcaaaaaactgttgCCATGAGAAGCCGATCCATTGGTGAATGTGCTCTGCCATCGGCCTATATTCGCAGTGCTAAAAGTGCTCCTGTTCTGATCCATACTTCCAAACCCTTCTTGCCTGATATTGTTCTCACTCCCCTTTCTGATGAGCTTTCAGatattgatgatgctcaaatccttccccgctcaaccagagtcagacatttttcacagagtgaagacactggaaatgaagtttttggtgctttgcatgaggaacagccactgcctcgaagtagcagcacttctgacatcttggaaccattcactgttgagcgagccaaagtaaataaagaggacacgagccccaaactgcctcctcttaatagtgagactggtggcaacagcgccaatgttcctgacctgatggatgagtttatcgcagagcgacttcgaagtggcaatgcctcgactatgacaagaagggggagtagtccaggcagccttgaaatcccgaaagacctccctgatattctaaacaagcagaaccagatgcggcctgttgatgaccctggtgtaccctccgaatggacgtctccagccagtgcagggagcagtgatctcatgagctcagatagtcactcggattccttcagtgccttccagtgtgagggacgaaaatttgacaactttggcttcgggactgacattgggatcccatcttctgctgatgtggatttaggttctggccatcatcaaagcactgaggagcaggaagtggccagcctaactacgcttcatttagattcagaaacaagcagccttaatcagcaagccttctctgcagaagttgcaaccgttactggttcagaaagtgcttcccccgttcattcagctctgggatccaggtcgcagaccccctccccatctacactgagcagagctcacatagaacagaaggacctacagctcgatgagaagctccaccactctgttcttcagacgccggatgacctagaaatcagtgaatttccatctgaatgttgtagcgtgatggcaggaggtactctcactgggtggcatgctg

Amino Acid Sequence

MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLSLQETQVTIEEVTPLVPPQSGDKGQEDLTSYFLEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQIRPKPHYVMIKKDAETNETIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESKNDTVDKVDKSAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEDTAITCSDIPCSETVADHDSAIEDGEKREEENGTSTSEHVRNSSWTKNGSYQEAFHVCEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKSMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLSDELSDIDDAQILPRSTRVRHFSQSEDTGNEVFGALHEEQPLPRSSSTSDILEPFTVERAKVNKEDTSPKLPPLNSETGGNSANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHSDSFSAFQCEGRKFDNFGFGTDIGIPSSADVDLGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVATVTGSESASPVHSALGSRSQTPSPSTLSRAHIEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHA

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU36891
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All RalGAP alpha plasmids MUST be grown at 30 degrees C or less to prevent recombination.