cDNA Clone RRI1

• Plasmid: pRS413-ADH Pr-HA-RRI1
• Parent Plasmid: pRS413
• Expressed: HA-RRI1
• Genbank: NM_001180276.1
• Tag: HA
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGTCACTTTCAAATAAGACTGTAAAGGAATTACGACAACTACT
AAAGGAAAGGTACACCGTGGAAGATGAACTAACTGAATCTATAGCGCTAT
CAAGTATGCGATTCAAGCCGTCGCAAGAACCTGAATTTCATGCGCTAAGC
CAATCTTCCTTGCTCAAAACTAAACTAAAACAACAAAGTAGTACAGATAT
TCCGAGTTACACCCACGTACTTATATCAAAATTATCATGTGAAAAGATCA
CCCATTATGCAGTTCGTGGAGGAAATATTGAAATCATGGGCATATTAATG
GGGTTCACATTAAAAGATAATATTGTTGTAATGGATTGTTTCAATCTTCC
CGTAGTAGGGACAGAAACTCGCGTAAATGCACAATTGGAGTCATACGAAT
ATATGGTTCAATATATCGACGAGATGTATAATCATAATGACGGTGGTGAT
GGGCGAGATTACAAGGGCGCTAAACTGAATGTTGTTGGCTGGTTCCATTC
ACATCCTGGTTACGACTGTTGGTTAAGTAATATAGATATCCAAACCCAGG
ATTTGAACCAGAGATTCCAAGATCCATATGTCGCAATTGTGGTCGATCCC
TTGAAAAGTCTGGAAGATAAAATTTTAAGAATGGGTGCATTTAGGACAAT
AGAAAGTAAAAGTGATGATAATAGTGCCACTTCATACTATGAACTAGAAA
CAATCATATTTGATTCAGAATTGAACCGAGCGCTATTTGAAACAAAGTTA
AACTTACATTGCGTAATAGAGGATGATGAGTCAGAACAAATATCATTAAA
TAGATTGATCGATTCAATGAAGCAGTATAGCTATTTGATGGATTCCAAAA
ATGTTAGAACAAGGATTAAGTTGGCTACTACCAGTGAACGTGTTAGCAAC
GAAAATAAAAAAAACATTGACTATCAAAACCGTTCAACAAGGTCTCAGTT
CTGCCTCAATACGCAAAGGGGGGATAGCACTGAAACAAGCTCCTTTGGAA
GCATGTTTTCAGGTGATAATACTAGTGACGTAGATATGGAAGATAGAAAC
CTTACCGAGTTCGACAGTACTGACACTAGTCTGTGCATAAACGGCGAACC
GAGCATTCATGTAAATCGCGTGGAAAGAAGCAGCAGGTCTACCGACAATT
TTCACAATTCTAAAAAACGCATGAACAGCAATCAGGAAAGATGTCATGAT
GAGGGCAATGATATGTTACAGAGAAATGTCTTGGAGACAGATTATGCAAG
AGCAAAAAATCGAATACTTGCGTCAAAGATAAAACAATATGAGCGGTTAC
GTTTTTACAAGGACACCTTTACATTATGAGAATTC

Amino Acid Sequence

MYPYDVPDYAGSMSLSNKTVKELRQLLKERYTVEDELTESIALSSMRFKP
SQEPEFHALSQSSLLKTKLKQQSSTDIPSYTHVLISKLSCEKITHYAVRG
GNIEIMGILMGFTLKDNIVVMDCFNLPVVGTETRVNAQLESYEYMVQYID
EMYNHNDGGDGRDYKGAKLNVVGWFHSHPGYDCWLSNIDIQTQDLNQRFQ
DPYVAIVVDPLKSLEDKILRMGAFRTIESKSDDNSATSYYELETIIFDSE
LNRALFETKLNLHCVIEDDESEQISLNRLIDSMKQYSYLMDSKNVRTRIK
LATTSERVSNENKKNIDYQNRSTRSQFCLNTQRGDSTETSSFGSMFSGDN
TSDVDMEDRNLTEFDSTDTSLCINGEPSIHVNRVERSSRSTDNFHNSKKR
MNSNQERCHDEGNDMLQRNVLETDYARAKNRILASKIKQYERLRFYKDTF
TL*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20974
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector for expressing N-terminally HA tagged RRI1 from the yeast ADH promoter; Estimated MW of expressed protein : 52172 Daltons; Parental SC20911 (pRS413-ADH promoter-HA tag)