cDNA Clone OTUD7B

• Plasmid: pGEX6P-1-OTUD7B
• Parent Plasmid: pGEX6P1
• Expressed: GST-OTUD7B
• Genbank: Q6GQQ9.1
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGACCCTGGACATGGATGCTGTTCTGTCAGATTTTGTCCGTTCCACAGGAGCAGAGCCAGGGCTAGCGCGAGATCTCCTAGAAGGAAAGAATTGGGATGTGAATGCCGCCCTCAGTGATTTTGAACAGCTACGTCAAGTCCATGCTGGAAACCTACCCCCATCCTTTAGTGAGGGGAGTGGTGGCTCCAGGACCCCTGAAAAAGGGTTTTCTGACAGAGAGCCTACTCGCCCTCCCCGACCCATCCTCCAGCGGCAGGATGACATCGTTCAAGAAAAACGCCTGTCTAGGGGCATCTCCCACGCCAGCTCCAGCATTGTTTCCCTGGCCCGGTCCCATGTCTCCTCCAATGGTGGGGGTGGGGGGAGCAATGAGCACCCCCTGGAAATGCCCATCTGTGCCTTCCAGCTTCCAGATCTCACTGTATACAATGAAGACTTCCGCAGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATGCTGGTTGCCTTGGAACAGGCAGGGCGTTTGAACTGGTGGGTGAGTGTGGATCCCACCTCTCAGAGGCTGCTTCCTTTGGCAACTACTGGAGATGGGAACTGCCTCCTGCATGCAGCCTCCCTTGGAATGTGGGGTTTCCATGATCGGGACTTGATGCTGCGGAAAGCTTTGTATGCACTGATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAAGGCGCTGGAGGTGGCAGCAGACACAGCAGAATAAAGAGTCAGGGCTGGTATACACAGAAGATGAATGGCAGAAGGAGTGGAATGAACTGATCAAGCTTGCCTCAAGTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGTGGTGGGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCACGTCTTTGTCCTTGCTCATGTGCTTAGGAGGCCCATAGTCGTCGTGGCAGACACCATGCTGAGGGACTCCGGAGGGGAAGCATTTGCCCCTATTCCCTTTGGAGGAATCTATCTGCCTTTGGAGGTCCCAGCCAGCCAGTGTCACCGCTCCCCTCTGGTGCTCGCCTATGATCAGGCCCACTTTTCTGCACTCGTGTCCATGGAGCAGAAGGAGAATACCAAGGAACAAGCTGTGATCCCACTTACAGATTCAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGTGGACCCTGGAAAGGGCTGGGAGTGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAGTGTAATTCTGTCCCTAGAGGTCAAATTGCATCTGCTGCATAGCTACATGAATGTGA
AGTGGATCCCACTGTCCTCTGATGCACAGGCTCCTCTGGCCCAGCCTGAGTCCCCCACCGCCTCAGCTGGAGATGAGCCCCGGTCCACTCCTGAGTCTGGAGACTCAGACAAGGAGTCAGTTGGCAGCAGTTCCACCAGCAACGAGGGCGGCCGGCGGAAGGAGAAGTCAAAGCGAGATCGGGAGAAGGACAAGAAGAGAGCAGATTCTGTGGCTAACAAACTGGGCAGCTTTGGCAAAACCTTGGGCAGCAAGCTCAAGAAGAACATGGGGGGCCTGATGCACAGCAAGGGTTCAAAGCCTGGAGGGGTGGGGACAGGGTTGGGAGGAAGCAGCGGCACTGAGACACTGGAGAAGAAGAAGAAAAACTCACTGAAGAGCTGGAAGGGTGGCAAGGAGGAGGCAGCTGGGGATGGGCCTGTGTCTGAGAAGCCCCCAGCTGAGTCTGTTGGTAACGGAGGGAGCAAGTATAGCCAGGAGGTGATGCAGAGCCTGAGCATTCTGAGGACTGCCATGCAAGGGGAGGGGAAGTTTATTTTTGTTGGAACCCTGAAGATGGGTCACCGTCACCAGTATCAGGAGGAAATGATCCAGCGCTACCTTTCTGATGCTGAGGAGAGATTCCTGGCAGAACAGAAGCAGAAGGAGGCAGAGAGGAAGATCATGAATGGAGGAATAGGGGGTGGCCCTCCTCCAGCCAAAAAGCCAGAGCCAGATGCTAGGGAAGAGCAGCCGACCGGTCCCCCAGCAGAGTCCAGGGCAATGGCATTTTCCACTGGCTACCCTGGGGACTTTACTATCCCTCGGCCGTCTGGGGGCGGAGTCCACTGCCAGGAACCCCGGAGGCAGTTGGCAGGGGGTCCATGTGTCGGGGGCCTACCACCATATGCCACCTTCCCCAGACAGTGCCCTCCTGGGCGACCCTACCCCCACCAGGACAGCATCCCTTCTCTGGAGCCAGGCAGCCACTCTAAGGATGGACTTCACAGGGGTGCCTTGTTACCACCCCCCTACCGAGTGGCTGATTCCTATAGCAATGGCTACAGAGAGCCCCCTGAGCCAGATGGATGGGCTGGAGGTCTCCGGGGCCTTCCCCCAACTCAGACCAAATGCAAACAACCGAACTGCAGCTTCTATGGACACCCTGAGACAAACAACTTCTGTTCCTGTTGTTACAGGGAAGAACTGAGGAGGAGGGAGCGGGAACCGGATGGGGAGCTCCTGGTGCACAGGTTCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMTLDMDAVLSDFVRST
GAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTP
EKGFSDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSS
NGGGGGSNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVALEQ
AGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKA
LYALMEKGVEKEALKRRWRWQQTQQNKESGLVYTEDEWQKEWNELIKLAS
SEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADT
MLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVSME
QKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDSDNVRLASVIL
SLEVKLHLLHSYMNVKWIPLSSDAQAPLAQPESPTASAGDEPRSTPESGD
SDKESVGSSSTSNEGGRRKEKSKRDREKDKKRADSVANKLGSFGKTLGSK
LKKNMGGLMHSKGSKPGGVGTGLGGSSGTETLEKKKKNSLKSWKGGKEEA
AGDGPVSEKPPAESVGNGGSKYSQEVMQSLSILRTAMQGEGKFIFVGTLK
MGHRHQYQEEMIQRYLSDAEERFLAEQKQKEAERKIMNGGIGGGPPPAKK
PEPDAREEQPTGPPAESRAMAFSTGYPGDFTIPRPSGGGVHCQEPRRQLA
GGPCVGGLPPYATFPRQCPPGRPYPHQDSIPSLEPGSHSKDGLHRGALLP
PPYRVADSYSNGYREPPEPDGWAGGLRGLPPTQTKCKQPNCSFYGHPETN
NFCSCCYREELRRREREPDGELLVHRF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20899
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for GST-OTUD7B