cDNA Clone UBA7

• Plasmid: pET28a-DAC-UBA7
• Parent Plasmid: pET28a-DAC (SC20568)
• Expressed: DAC-UBA7
• Genbank: NP_003326.2
• Tag: DAC
• Species: Human

Sequence of Insert

AGATCTATGGATGCCCTGGACGCTTCGAAGCTACTGGATGAGGAGCTGTA
TTCAAGACAGCTGTATGTGCTGGGCTCACCTGCCATGCAGAGGATTCAGG
GAGCCAGGGTCCTGGTGTCAGGCCTGCAGGGCCTGGGGGCCGAGGTGGCC
AAGAACTTGGTTCTGATGGGTGTGGGCAGCCTCACTCTGCATGATCCCCA
CCCCACCTGCTGGTCCGACCTGGCTGCCCAGTTTCTCCTCTCAGAGCAGG
ACTTGGAAAGGAGCAGAGCCGAGGCCTCTCAAGAGCTCTTGGCTCAGCTC
AACAGAGCTGTCCAGGTCGTCGTGCACACGGGTGACATCACTGAGGACCT
GCTGTTGGACTTCCAGGTGGTGGTGCTGACTGCTGCAAAGCTGGAGGAGC
AGCTGAAGGTGGGCACCTTGTGTCATAAGCATGGAGTTTGCTTTCTGGCG
GCTGACACCCGGGGCCTCGTGGGGCAGTTGTTCTGTGACTTTGGTGAGGA
CTTCACTGTGCAGGACCCCACAGAGGCAGAACCCCTGACAGCTGCCATCC
AGCACATCTCCCAGGGCTCCCCTGGCATTCTCACTCTGAGGAAAGGGGCC
AATACCCACTACTTCCGTGATGGAGACTTGGTGACTTTCTCGGGAATTGA
GGGAATGGTTGAGCTCAACGACTGTGATCCCCGGTCTATCCACGTGCGGG
AGGATGGGTCCCTGGAGATTGGAGACACAACAACTTTCTCTCGGTACTTG
CGTGGTGGGGCTATCACTGAAGTCAAGAGACCCAAGACTGTGAGACATAA
GTCCCTGGACACAGCCCTGCTCCAGCCCCATGTGGTGGCCCAGAGCTCCC
AGGAAGTTCACCATGCCCACTGCCTGCATCAGGCCTTCTGTGCACTGCAC
AAGTTCCAGCACCTCCATGGCCGGCCACCCCAGCCCTGGGATCCTGTTGA
TGCAGAGACTGTGGTGGGCCTGGCCCGGGACCTGGAACCACTGAAGCGGA
CAGAGGAAGAGCCACTGGAAGAGCCACTGGATGAGGCCCTAGTGCGGACA
GTCGCCCTAAGCAGTGCAGGTGTCTTGAGCCCTATGGTGGCCATGCTGGG
TGCAGTAGCTGCCCAGGAAGTGCTGAAGGCAATCTCCAGGAAGTTCATGC
CTCTGGACCAGTGGCTTTACTTTGATGCCCTCGATTGTCTTCCGGAAGAT
GGGGAGCTCCTTCCCAGTCCTGAGGACTGTGCCCTGAGAGGCAGCCGCTA
TGATGGGCAAATTGCAGTGTTTGGGGCTGGTTTTCAGGAGAAACTGAGAC
GCCAGCACTACCTCCTGGTGGGCGCTGGTGCCATTGGTTGTGAGCTGCTC
AAAGTCTTTGCCCTAGTGGGACTGGGGGCCGGGAACAGCGGGGGCTTGAC
TGTTGTTGACATGGACCACATAGAGCGCTCCAATCTCAGCCGTCAGTTCC
TCTTCAGGTCCCAGGACGTTGGTAGACCCAAGGCAGAGGTGGCTGCAGCA
GCTGCCCGGGGCCTGAACCCAGACTTACAGGTGATCCCGCTCACCTACCC
ACTGGATCCCACCACAGAGCACATCTATGGGGATAACTTTTTCTCCCGTG
TGGATGGTGTGGCTGCTGCCCTGGACAGTTTCCAGGCCCGGCGCTATGTG
GCTGCTCGTTGCACCCACTATCTGAAGCCACTGCTGGAGGCAGGCACATC
GGGCACCTGGGGCAGTGCTACAGTATTCATGCCACATGTGACTGAGGCCT
ACAGAGCCCCTGCCTCAGCTGCAGCTTCTGAGGATGCCCCCTACCCTGTC
TGTACCGTGCGGTACTTCCCTAGCACAGCCGAGCACACCCTGCAGTGGGC
CCGGCATGAGTTTGAAGAACTCTTCCGACTGTCTGCAGAGACCATCAACC
ACCACCAACAGGCACACACTTCCCTGGCAGACATGGATGAGCCACAGACA
CTCACCTTACTGAAGCCAGTGCTTGGGGTCCTGAGAGTGCGTCCACAGAA
CTGGCAAGACTGTGTGGCGTGGGCTCTTGGCCACTGGAAACTCTGCTTTC
ATTATGGCATCAAACAGCTGCTGAGGCACTTCCCACCTAATAAAGTGCTT
GAGGATGGAACTCCCTTCTGGTCAGGTCCCAAACAGTGTCCCCAGCCCTT
GGAGTTTGACACCAACCAAGACACACACCTCCTCTACGTACTGGCAGCTG
CCAACCTGTATGCCCAGATGCATGGGCTGCCTGGCTCACAGGACTGGACT
GCACTCAGGGAGCTGCTGAAGCTGCTGCCACAGCCTGACCCCCAACAGAT
GGCCCCCATCTTTGCTAGTAATCTAGAGCTGGCTTCGGCTTCTGCTGAGT
TTGGCCCTGAGCAGCAGAAGGAACTGAACAAAGCCCTGGAAGTCTGGAGT
GTGGGCCCTCCCCTGAAGCCTCTGATGTTTGAGAAGGATGATGACAGCAA
CTTCCATGTGGACTTTGTGGTAGCGGCAGCTAGCCTGAGATGTCAGAACT
ACGGGATTCCACCGGTCAACCGTGCCCAGAGCAAGCGAATTGTGGGCCAG
ATTATCCCAGCCATTGCCACCACTACAGCAGCTGTGGCAGGCCTGTTGGG
CCTGGAGCTGTATAAGGTGGTGAGTGGGCCACGGCCTCGTAGTGCCTTTC
GCCACAGCTACCTACATCTGGCTGAAAACTACCTCATCCGCTATATGCCT
TTTGCCCCAGCCATCCAGACGTTCCATCACCTGAAGTGGACCTCTTGGGA
CCGTCTGAAGGTACCAGCTGGGCAGCCTGAGAGGACCCTGGAGTCGCTGC
TGGCTCATCTTCAGGAGCAGCACGGGTTGAGGGTGAGGATCCTGCTGCAC
GGCTCAGCCCTGCTCTATGCGGCCGGATGGTCACCTGAAAAGCAGGCCCA
GCACCTGCCCCTCAGGGTGACAGAACTGGTTCAGCAGCTGACAGGCCAGG
CACCTGCTCCTGGGCAGCGGGTGTTGGTGCTAGAGCTGAGCTGTGAGGGT
GACGACGAGGACACTGCCTTCCCACCTCTGCACTATGAGCTGTGAGCGGC
CGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPLVPRGSGSMDALDASKLLDEELYSRQLYVLGSPAMQR
IQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLS
EQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKL
EEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTA
AIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIH
VREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQ
SSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPL
KRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRK
FMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEK
LRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSR
QFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFF
SRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVT
EAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAET
INHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKL
CFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVL
AAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASAS
AEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRC
QNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRS
AFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLE
SLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLT
GQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL*



MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPLVPRGSGS

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU20895
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression vector for UBA7 with N-terminal DAC tag (and thrombin cleavage site); ; Estimated MW of expressed protein : 141067 Daltons