cDNA Clone OTUB1

• Plasmid: pcDNA5-FRT/TO-GFP OTUB1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-OTUB1
• Genbank: NM_017670
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCGGCGGAGGAACCTCAGCAGCAGAAGCAGGAGCCGCTGGG
CAGCGACTCCGAAGGTGTTAACTGTCTGGCCTATGATGAAGCCATCATGG
CTCAGCAGGACCGAATTCAGCAAGAGATTGCTGTGCAGAACCCTCTGGTG
TCAGAGCGGCTGGAGCTCTCGGTCCTATACAAGGAGTATGCTGAAGATGA
CAACATCTATCAACAGAAGATCAAGGACCTCCACAAAAAGTACTCGTACA
TCCGCAAGACCAGGCCTGACGGCAACTGTTTCTATCGGGCTTTCGGATTC
TCCCACTTGGAGGCACTGCTGGATGACAGCAAGGAGTTGCAGCGGTTCAA
GGCTGTGTCTGCCAAGAGCAAGGAAGACCTGGTGTCCCAGGGCTTCACTG
AATTCACAATTGAGGATTTCCACAACACGTTCATGGACCTGATTGAGCAG
GTGGAGAAGCAGACCTCTGTCGCCGACCTGCTGGCCTCCTTCAATGACCA
GAGCACCTCCGACTACCTTGTGGTCTACCTGCGGCTGCTCACCTCGGGCT
ACCTGCAGCGCGAGAGCAAGTTCTTCGAGCACTTCATCGAGGGTGGACGG
ACTGTCAAGGAGTTCTGCCAGCAGGAGGTGGAGCCCATGTGCAAGGAGAG
CGACCACATCCACATCATTGCGCTGGCCCAGGCCCTCAGCGTGTCCATCC
AGGTGGAGTACATGGACCGCGGCGAGGGCGGCACCACCAATCCGCACATC
TTCCCTGAGGGCTCCGAGCCCAAGGTCTACCTTCTCTACCGGCCTGGACA
CTACGATATCCTCTACAAATAGGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAAEEP
QQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSV
LYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLD
DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVA
DLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQ
EVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPK
VYLLYRPGHYDILYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20859
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP-tagged OTUB1 for mammalian expression Flp-In vector; ; Estimated MW of expressed protein : 58609 Daltons