cDNA Clone Ubiquitin

• Plasmid: pHis-TEV30a-YFP Venus-Ubiquitin
• Parent Plasmid: pHIS TEV30a YFP
• Expressed: YFP-Ubiquitin
• Genbank: P62988
• Tag: YFP

Sequence of Insert

GGATCCGGCTCCGGCTCCATGCAGATCTTCGTGAAGACCCTGACTGGTAA
GACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGG
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC
TTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACAT
CCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGAT
AAGAATTC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICT
TGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSGSGSMQIFV
KTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRT
LSDYNIQKESTLHLVLRLRGG**

• Antibiotic: Amp
• DU Number: DU20857
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial YFP Venus ubiquitin. No 6His (YFP subcloned into Nco1 site of pHISTEV30a). Vector obtained from Hay group